protein coding gene - spi1 (SPBC1289.03c) - Ran GTPase Spi1

Gene summary

Standard name

spi1

Systematic ID

SPBC1289.03c

Product

Ran GTPase Spi1

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P28748

ORFeome ID

40/40B10

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 4386149..4387361 reverse strand

Annotation

Protein features

Orthologs

• RAN (HGNC:9846) Homo sapiens
• spi1 (SJAG_01449) Schizosaccharomyces japonicus
• GSP1 (YLR293C) Saccharomyces cerevisiae
• GSP2 (YOR185C) Saccharomyces cerevisiae

References / Literature

PMID:19680239 - Structures of the tRNA export factor in the nuclear and cytosolic states.

Cook AG et al. Nature 2009 Sep 03;461(7260):60-5

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:9504913 - The identification of cDNAs that affect the mitosis-to-interphase transition in Schizosaccharomyces pombe, including sbp1, which encodes a spi1p-GTP-binding protein.

He X et al. Genetics 1998 Feb;148(2):645-56

PMID:7987414 - Phenotype of the fission yeast cell cycle regulatory mutant pim1-46 is suppressed by a tobacco cDNA encoding a small, Ran-like GTP-binding protein.

Merkle T et al. Plant J 1994 Oct;6(4):555-65

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Ren L et al. PLoS One 2011 Feb 28;6(2):e16719

PMID:16371130 - Loss of RanGEF/Pim1 activity abolishes the orchestration of Ran-mediated mitotic cellular events in S. pombe.

Hirose E et al. Genes Cells 2006 Jan;11(1):29-46

PMID:1855255 - Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase.

Matsumoto T et al. Cell 1991 Jul 26;66(2):347-60

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:18600047 - Genome-wide identification of haploinsufficiency in fission yeast.

Baek ST et al. J Microbiol Biotechnol 2008 Jun;18(6):1059-63

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.

Egan ED et al. RNA 2014 Jun;20(6):867-81

PMID:15371542 - The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association.

Kerres A et al. Mol Biol Cell 2004 Dec;15(12):5255-67

PMID:18204818 - Identification of small molecules inducing apoptosis by cell-based assay using fission yeast deletion mutants.

Chung KS et al. Invest New Drugs 2008 Aug;26(4):299-307

PMID:11086011 - The fission yeast ran GTPase is required for microtubule integrity.

Fleig U et al. J Cell Biol 2000 Nov 27;151(5):1101-11

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:1844237 - Spi1 GTPase interacts with RCC1 to maintain interdependency of cell cycle events.

Matsumoto T et al. Princess Takamatsu Symp 1991;22:145-52

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:8887664 - Perturbations in the spi1p GTPase cycle of Schizosaccharomyces pombe through its GTPase-activating protein and guanine nucleotide exchange factor components result in similar phenotypic consequences.

Matynia A et al. Mol Cell Biol 1996 Nov;16(11):6352-62

PMID:12376568 - An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin.

Tange Y et al. J Cell Sci 2002 Nov 15;115(Pt 22):4375-85

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model

PMID:10394366 - The crystal structure of rna1p: a new fold for a GTPase-activating protein.

Hillig RC et al. Mol Cell 1999 Jun;3(6):781-91

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.

Anver S et al. EMBO Rep 2024 Oct 02;

PMID:9560394 - Isolation and characterization of fission yeast sns mutants defective at the mitosis-to-interphase transition.

Matynia A et al. Genetics 1998 Apr;148(4):1799-811

PMID:17476213 - Alp7/TACC is a crucial target in Ran-GTPase-dependent spindle formation in fission yeast.

Sato M et al. Nature 2007 May 17;447(7142):334-7

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:18422602 - Schizosaccharomyces pombe Snf2SR, a novel SNF2 family protein, interacts with Ran GTPase and modulates both RanGEF and RanGAP activities.

Ohba T et al. Genes Cells 2008 Jun;13(6):571-82

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:8896453 - Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1.

Noguchi E et al. EMBO J 1996 Oct 15;15(20):5595-605

PMID:17651922 - Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction.

Oki M et al. Gene 2007 Oct 01;400(1-2):114-21

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:8313905 - A fission yeast RCC1-related protein is required for the mitosis to interphase transition.

Sazer S et al. EMBO J 1994 Feb 01;13(3):606-15

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.

Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:11290708 - Fission yeast Mog1p homologue, which interacts with the small GTPase Ran, is required for mitosis-to-interphase transition and poly(A)(+) RNA metabolism.

Tatebayashi K et al. Genetics 2001 Apr;157(4):1513-22

PMID:8387358 - Interaction of the pim1/spi1 mitotic checkpoint with a protein phosphatase.

Matsumoto T et al. Mol Biol Cell 1993 Mar;4(3):337-45