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protein coding gene - pob1 (SPBC1289.04c) - Boi family protein

Gene summary

Standard name
pob1
Systematic ID
SPBC1289.04c
Product
Boi family protein
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74653
ORFeome ID
33/33H12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4387796..4391162 reverse strand

Annotation

Complementation

PBO:0016098 - functionally complemented by S. cerevisiae BOI2

References:

Disease association

MONDO:0030909 - intellectual disability, X-linked, syndromic, Houge type

References:

GO biological process

GO:0006887 - exocytosis

References:

GO:0120009 - intermembrane lipid transfer

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GO cellular component

GO:0051285 - cell cortex of cell tip

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GO:0032153 - cell division site

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GO:0051286 - cell tip

References:

GO:0031097 - medial cortex

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GO molecular function

GO:0008289 - lipid binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:00696 - phosphorylated residue

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001886 - meiotic cell cycle entry and sporulation in haploid

References:

Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

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Genotypes:

FYPO:0003209 - abolished protein localization to cell tip, with protein mislocalized to cytoplasm

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Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

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Genotypes:

FYPO:0004572 - decreased exocytosis during vegetative growth

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Genotypes:

FYPO:0003770 - decreased transcriptional response to pheromone

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Genotypes:

FYPO:0006548 - increased gene expression

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Genotypes:

FYPO:0001991 - inviable after spore germination, without cell division

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Genotypes:

FYPO:0003501 - inviable aseptate mononucleate vegetative cell, normal cell length

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Genotypes:

FYPO:0003333 - inviable lemon-shaped cell

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Genotypes:

FYPO:0002187 - inviable normal volume spheroid vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002674 - normal protein localization to plasma membrane

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Genotypes:

FYPO:0003414 - normal protein localization to septum

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0007514 - sensitive to manumycin A

References:

Genotypes:

FYPO:0000129 - spherical vegetative cell

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00018SH3_1SH3_domainPfam
PF00169PHPH_domainPfam
PF07647SAM_2SAMPfam
cd00174SH3CDD
cd09535SAM_BOI-like_fungalCDD
cd13316PH_BoiCDD
PS50002SH3SH3_domainPROSITE profiles
PS50003PH_DOMAINPH_domainPROSITE profiles
PS50105SAM_DOMAINSAMPROSITE profiles
SM00233PHPH_domainSMART
SM00326SH3SH3_domainSMART
SM00454SAMSAMSMART
G3DSA:1.10.150.50SAM/pointed_sfCATH-Gene3D
G3DSA:2.30.29.30PH-like_dom_sfCATH-Gene3D
G3DSA:2.30.30.40CATH-Gene3D
SSF47769SAM/pointed_sfSUPERFAMILY
SSF50044SH3-like_dom_sfSUPERFAMILY
SSF50729SUPERFAMILY
PTHR22902Boi1/Boi2-likePANTHER
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte

Orthologs

References / Literature

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:19710424 - Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Rincón SA et al. Mol Biol Cell 2009 Oct;20(20):4390-9
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:25500221 - Balance between exocytosis and endocytosis determines the efficacy of sterol-targeting antibiotics.
Nishimura S et al. Chem Biol 2014 Dec 18;21(12):1690-9
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PANTHER:PTHR12844 - Unknown title
GO_REF:0000108 - Automatic assignment of GO terms using logical inference, based on on inter-ontology links.
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.
Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9
PMID:24147005 - The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis.
Weston C et al. PLoS One 2013;8(10):e77487
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:32415063 - Conserved protein Pir2 ARS2 mediates gene repression through cryptic introns in lncRNAs.
Thillainadesan G et al. Nat Commun 2020 May 15;11(1):2412
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:12206652 - Mac1, a fission yeast transmembrane protein localizing to the poles and septum, is required for correct cell separation at high temperatures.
Grandin N et al. Biol Cell 2002 Jun;94(3):127-37
PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.
Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:10436025 - Fission yeast Pob1p, which is homologous to budding yeast Boi proteins and exhibits subcellular localization close to actin patches, is essential for cell elongation and separation.
Toya M et al. Mol Biol Cell 1999 Aug;10(8):2745-57
PMID:20739711 - Cooperation between the septins and the actomyosin ring and role of a cell-integrity pathway during cell division in fission yeast.
Wu JQ et al. Genetics 2010 Nov;186(3):897-915
PMID:21899677 - Cdc42 regulates multiple membrane traffic events in fission yeast.
Estravís M et al. Traffic 2011 Dec;12(12):1744-58
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:21401840 - Pob1 ensures cylindrical cell shape by coupling two distinct rho signaling events during secretory vesicle targeting.
Nakano K et al. Traffic 2011 Jun;12(6):726-39
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29735745 - Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1.
Schiklenk C et al. J Cell Biol 2018 Jul 02;217(7):2383-2401
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:28784611 - Phosphoinositide-mediated ring anchoring resists perpendicular forces to promote medial cytokinesis.
Snider CE et al. J Cell Biol 2017 Oct 02;216(10):3041-3050
PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.
Chen JS et al. Yeast 2016 Sep;33(9):507-17
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41