protein coding gene - gfa1 (SPBC12C2.11) - glutamine-fructose-6-phosphate transaminase Gfa1

Gene summary

Standard name: gfa1
Systematic ID: SPBC12C2.11
Product: glutamine-fructose-6-phosphate transaminase Gfa1
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: SPBC21D10.02
UniProt ID: Q09740
ORFeome ID: 38/38C11
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome II: 2448092..2450914 reverse strand

Annotation

PBO:0004067 - 2.6.1.16

Disease association

MONDO:0012518 - congenital myasthenic syndrome 12

References:

PB_REF:0000006

GO biological process

GO:0006048 - UDP-N-acetylglucosamine biosynthetic process

References:

GO_REF:0000033

GO cellular component

GO:0005829 - cytosol

References:

PMID:16823372

GO molecular function

GO:0097367 - carbohydrate derivative binding

References:

GO_REF:0000002

GO:0004360 - L-glutamine:D-fructose-6-phosphate transaminase (isomerizing) activity

References:

GO_REF:0000033

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

PMID:23697806

Genotypes:

gfa1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

gfa1delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

ID	Name	InterPro name	DB name
PF01380	SIS	SIS_dom	Pfam
PF13537	GATase_7	GATase_2_dom	Pfam
cd00714	GFAT	GFAT_N	CDD
cd05008	SIS_GlmS_GlmD_1	GlmS/AgaS_SIS	CDD
cd05009	SIS_GlmS_GlmD_2	GlmS/FrlB_SIS	CDD
PS51278	GATASE_TYPE_2	GATase_2_dom	PROSITE profiles
PS51464	SIS	SIS_dom	PROSITE profiles
G3DSA:3.40.50.10490:FF:000001			CATH-FunFam
G3DSA:3.40.50.10490:FF:000002			CATH-FunFam
G3DSA:3.40.50.10490			CATH-Gene3D
G3DSA:3.60.20.10		Ntn_hydrolases_N	CATH-Gene3D
SSF53697		SIS_dom_sf	SUPERFAMILY
SSF56235		Ntn_hydrolases_N	SUPERFAMILY
PTHR10937			PANTHER
NF001484	PRK00331.1		NCBIFAM

Orthologs

GFPT1 (HGNC:4241) Homo sapiens
GFPT2 (HGNC:4242) Homo sapiens
gfa1 (SJAG_04728) Schizosaccharomyces japonicus
GFA1 (YKL104C) Saccharomyces cerevisiae
YMR084W Saccharomyces cerevisiae

References / Literature

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;