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protein coding gene - glo1 (SPBC12C2.12c) - lactoylglutathione lyase (glyoxalase I)

Gene summary

Standard name
glo1
Systematic ID
SPBC12C2.12c
Product
lactoylglutathione lyase (glyoxalase I)
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBC21D10.03c
UniProt ID
Q09751
ORFeome ID
12/12E09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2446557..2447857 forward strand

Annotation

PBO:0004064 - 4.4.1.5

Catalytic activity attributes

PBO:0091784 - kcat/KM 4.3x10^7 M-1 min-1

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GO biological process

GO:1990748 - cellular detoxification

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GO:0071470 - cellular response to osmotic stress

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0004462 - lactoylglutathione lyase activity

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GO:0046872 - metal ion binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008421 - abolished lactoylglutathione lyase activity

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Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0000636 - increased cell population growth rate

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Genotypes:

FYPO:0004326 - increased duration of protein phosphorylation during cellular response to methylglyoxal

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Genotypes:

FYPO:0004351 - increased protein localization to nucleus during cellular response to methylglyoxal

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Genotypes:

FYPO:0001309 - increased viability in stationary phase

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Genotypes:

FYPO:0001788 - normal protein localization to cytoplasm

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Genotypes:

FYPO:0004154 - normal protein phosphorylation during cellular response to heat

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Genotypes:

FYPO:0004327 - normal protein phosphorylation during cellular response to sorbitol

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Genotypes:

FYPO:0009031 - resistance to bleomycin

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0003407 - resistance to glyoxal

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0003406 - resistance to methylglyoxal

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0003408 - sensitive to methylglyoxal

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Subunit composition

PBO:0001467 - monomeric

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Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00903GlyoxalaseGlyas_Fos-R_dOase_domPfam
cd07233GlxI_ZnCDD
PS00934GLYOXALASE_I_1Glyoxalase_1_CSPROSITE patterns
PS00935GLYOXALASE_I_2Glyoxalase_1_CSPROSITE patterns
PS51819VOCVOC_corePROSITE profiles
G3DSA:3.10.180.10Glyas_Bleomycin-R_OHBP_DaseCATH-Gene3D
SSF54593Glyas_Bleomycin-R_OHBP_DaseSUPERFAMILY
PTHR10374PANTHER
TIGR00068glyox_IGlyoxalase_1NCBIFAM

Orthologs

References / Literature

PMID:16141205 - The glycolytic metabolite methylglyoxal activates Pap1 and Sty1 stress responses in Schizosaccharomyces pombe.
Zuin A et al. J Biol Chem 2005 Nov 04;280(44):36708-13
PMID:23231582 - Genome-wide characterization of the phosphate starvation response in Schizosaccharomyces pombe.
Carter-O'Connell I et al. BMC Genomics 2012 Dec 12;13:697
PMID:24758716 - Identification of glutathione (GSH)-independent glyoxalase III from Schizosaccharomyces pombe.
Zhao Q et al. BMC Evol Biol 2014 Apr 23;14:86
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:28357272 - A central role for TOR signalling in a yeast model for juvenile CLN3 disease.
Bond ME et al. Microb Cell 2015 Nov 11;2(12):466-480
GO_REF:0000051 - S. pombe keyword mapping
PMID:15042280 - Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombe.
Takatsume Y et al. Arch Microbiol 2004 May;181(5):371-7
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:17919454 - Modulation of Spc1 stress-activated protein kinase activity by methylglyoxal through inhibition of protein phosphatase in the fission yeast Schizosaccharomyces pombe.
Takatsume Y et al. Biochem Biophys Res Commun 2007 Nov 30;363(4):942-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11859360 - The genome sequence of Schizosaccharomyces pombe.
Wood V et al. Nature 2002 Feb 21;415(6874):871-80
PMID:15714295 - Unique regulation of glyoxalase I activity during osmotic stress response in the fission yeast Schizosaccharomyces pombe: neither the mRNA nor the protein level of glyoxalase I increase under conditions that enhance its activity.
Takatsume Y et al. Arch Microbiol 2005 Mar;183(3):224-7
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
GO_REF:0000002 - Comments
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)