protein coding gene - sad1 (SPBC12D12.01) - spindle pole body SUN domain protein Sad1

Gene summary

Standard name

sad1

Systematic ID

SPBC12D12.01

Product

spindle pole body SUN domain protein Sad1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

SPBC16H5.01c

UniProt ID

Q09825

ORFeome ID

33/33B06

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2303766..2306661 forward strand

Annotation

Protein features

Orthologs

• SPAG4 (HGNC:11214) Homo sapiens
• SUN2 (HGNC:14210) Homo sapiens
• SUN5 (HGNC:16252) Homo sapiens
• SUN1 (HGNC:18587) Homo sapiens
• SUN3 (HGNC:22429) Homo sapiens
• sad1 (SJAG_05196) Schizosaccharomyces japonicus
• MPS3 (YJL019W) Saccharomyces cerevisiae

References / Literature

PMID:27889481 - Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.

Fernández-Álvarez A et al. Dev Cell 2016 Dec 05;39(5):544-559

PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2

PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.

Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477

PMID:38773107 - The SUN-family protein Sad1 mediates heterochromatin spatial organization through interaction with histone H2A-H2B.

Sun W et al. Nat Commun 2024 May 21;15(1):4322

PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33

PMID:22042620 - Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle.

Tamm T et al. J Cell Biol 2011 Oct 31;195(3):467-84

PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.

Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:34346498 - Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.

Pineda-Santaella A et al. J Cell Sci 2021 Aug 15;134(16)

PMID:39833200 - Centromere positioning orchestrates telomere bouquet formation and the initiation of meiotic differentiation.

Jiménez-Martín A et al. Nat Commun 2025 Jan 20;16(1):837

PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.

Wu JQ et al. Science 2005 Oct 14;310(5746):310-4

PMID:30503780 - The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope.

Hu C et al. Structure 2019 Feb 05;27(2):335-343.e3

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:15047861 - Loss of Apm1, the micro1 subunit of the clathrin-associated adaptor-protein-1 complex, causes distinct phenotypes and synthetic lethality with calcineurin deletion in fission yeast.

Kita A et al. Mol Biol Cell 2004 Jun;15(6):2920-31

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:24963130 - The KASH protein Kms2 coordinates mitotic remodeling of the spindle pole body.

Wälde S et al. J Cell Sci 2014 Aug 15;127(Pt 16):3625-40

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:23649273 - Fission yeast leucine-rich repeat protein Lrp1 is essential for cell morphogenesis as a component of the morphogenesis Orb6 network (MOR).

Kume K et al. Biosci Biotechnol Biochem 2013;77(5):1086-91

PMID:37714149 - Direct recruitment of Mis18 to interphase spindle pole bodies promotes CENP-A chromatin assembly.

London N et al. Curr Biol 2023 Oct 09;33(19):4187-4201.e6

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:20967237 - Fission yeast cells undergo nuclear division in the absence of spindle microtubules.

Castagnetti S et al. PLoS Biol 2010 Oct 12;8(10):e1000512

PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.

Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.

Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16

PMID:21880100 - Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres.

Hiraoka Y et al. Genes Cells 2011 Oct;16(10):1000-11

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:27611693 - A Taz1- and Microtubule-Dependent Regulatory Relationship between Telomere and Centromere Positions in Bouquet Formation Secures Proper Meiotic Divisions.

Katsumata K et al. PLoS Genet 2016 Sep;12(9):e1006304

PMID:34080538 - Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.

Krüger LK et al. Elife 2021 Jun 03;10

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:20833892 - Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane.

Itadani A et al. Eukaryot Cell 2010 Dec;9(12):1925-35

PMID:19948484 - Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation.

Chikashige Y et al. J Cell Biol 2009 Nov 02;187(3):413-27

PMID:34133218 - Redistribution of centrosomal proteins by centromeres and Polo kinase controls partial nuclear envelope breakdown in fission yeast.

Bestul AJ et al. Mol Biol Cell 2021 Aug 01;32(16):1487-1500

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:23084836 - Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.

Tanaka A et al. Mol Cell 2012 Nov 30;48(4):532-46

PMID:12857865 - Fission yeast cdc31p is a component of the half-bridge and controls SPB duplication.

Paoletti A et al. Mol Biol Cell 2003 Jul;14(7):2793-808

PMID:28619713 - Molecular model of fission yeast centrosome assembly determined by superresolution imaging.

Bestul AJ et al. J Cell Biol 2017 Aug 07;216(8):2409-2424

PMID:30759079 - A systematic genetic screen identifies essential factors involved in nuclear size control.

Cantwell H et al. PLoS Genet 2019 Feb;15(2):e1007929

PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.

Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80

PMID:25253718 - csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.

Costa J et al. Mol Biol Cell 2014 Dec 01;25(24):3900-8

PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.

UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531

PMID:9802907 - Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle.

Nabeshima K et al. Mol Biol Cell 1998 Nov;9(11):3211-25

PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.

Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69

PMID:22438582 - Spindle pole body components are reorganized during fission yeast meiosis.

Ohta M et al. Mol Biol Cell 2012 May;23(10):1799-811

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:31015410 - Nuclear membrane protein Lem2 regulates nuclear size through membrane flow.

Kume K et al. Nat Commun 2019 Apr 23;10(1):1871

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:9420333 - Asymmetric segregation on spindle poles of the Schizosaccharomyces pombe septum-inducing protein kinase Cdc7p.

Sohrmann M et al. Genes Dev 1998 Jan 01;12(1):84-94

PMID:7744953 - The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability.

Hagan I et al. J Cell Biol 1995 May;129(4):1033-47

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.

Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8

PMID:22174761 - Roles of the DYRK kinase Pom2 in cytokinesis, mitochondrial morphology, and sporulation in fission yeast.

Wu P et al. PLoS One 2011;6(12):e28000

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:14655046 - Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast.

Miki F et al. Mol Genet Genomics 2004 Jan;270(6):449-61

PMID:30462301 - Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.

Hu C et al. Nucleic Acids Res 2019 Feb 20;47(3):1573-1584

PMID:23669133 - Csi1 illuminates the mechanism and function of Rabl configuration.

Hou H et al. Nucleus 2013;4(3):176-81

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:24943839 - A role for nuclear envelope-bridging complexes in homology-directed repair.

Swartz RK et al. Mol Biol Cell 2014 Aug 15;25(16):2461-71