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protein coding gene - ned8 (SPBC12D12.08c) - ubiquitin-like protein modifier for cullin Ned8

Gene summary

Standard name
ned8
Systematic ID
SPBC12D12.08c
Product
ubiquitin-like protein modifier for cullin Ned8
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
nedd8, ubl1, SPBC24C6.01c
UniProt ID
O14399
ORFeome ID
03/03B06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2319758..2320295 reverse strand

Annotation

GO biological process

GO:0019941 - modification-dependent protein catabolic process

References:

GO:0045116 - protein neddylation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0019005 - SCF ubiquitin ligase complex

References:

GO molecular function

GO:0031386 - protein tag activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111862 - ubiquitin family protein

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00240ubiquitinUbiquitin-like_domPFAM
cd01806Ubl_NEDD8Nedd8-likeCDD
PS00299UBIQUITIN_1Ubiquitin_CSPROSITE_PATTERNS
PS50053UBIQUITIN_2Ubiquitin-like_domPROSITE_PROFILES
SM00213ubq_7Ubiquitin-like_domSMART
PR00348UBIQUITINUbiquitin_domPRINTS
G3DSA:3.10.20.90:FF:000023FUNFAM
SSF54236Ubiquitin-likeUbiquitin-like_domsfSUPERFAMILY
G3DSA:3.10.20.90GENE3D
PTHR10666UBIQUITINUbiquitin_ubiquitin-likePANTHER

Orthologs

References / Literature

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:16252005 - Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.
Liu C et al. EMBO J 2005 Nov 16;24(22):3940-51
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23496905 - Nedd8 processing enzymes in Schizosaccharomyces pombe.
O'Donoghue JE et al. BMC Biochem 2013 Mar 15;14:8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:11854407 - Deletion mutants in COP9/signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes.
Mundt KE et al. Mol Biol Cell 2002 Feb;13(2):493-502
PMID:20211136 - Stc1: a critical link between RNAi and chromatin modification required for heterochromatin integrity.
Bayne EH et al. Cell 2010 Mar 05;140(5):666-77
PMID:17114925 - A cullin E3 ubiquitin ligase complex associates with Rik1 and the Clr4 histone H3-K9 methyltransferase and is required for RNAi-mediated heterochromatin formation.
Hong EJ et al. RNA Biol 2005;2(3):106-11
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:11504566 - The fission yeast COP9/signalosome is involved in cullin modification by ubiquitin-related Ned8p.
Zhou C et al. BMC Biochem 2001;2:7
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:10880460 - Covalent modifier NEDD8 is essential for SCF ubiquitin-ligase in fission yeast.
Osaka F et al. EMBO J 2000 Jul 03;19(13):3475-84
PMID:14527422 - BTB/POZ domain proteins are putative substrate adaptors for cullin 3 ubiquitin ligases.
Geyer R et al. Mol Cell 2003 Sep;12(3):783-90
PMID:14623327 - But1 and But2 proteins bind to Uba3, a catalytic subunit of E1 for neddylation, in fission yeast.
Yashiroda H et al. Biochem Biophys Res Commun 2003 Nov 21;311(3):691-5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83