PomBase home

protein coding gene - trm734 (SPBC1306.02) - tRNA (guanosine 34-2'-O)-methyltransferase regulator Trm734

Gene summary

Standard name
trm734
Systematic ID
SPBC1306.02
Product
tRNA (guanosine 34-2'-O)-methyltransferase regulator Trm734
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBC4.08, rtt10
UniProt ID
Q9USZ0
ORFeome ID
47/47G11
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1180847..1184250 forward strand

Annotation

GO biological process

GO:0030488 - tRNA methylation

References:

GO:0002130 - wobble position ribose methylation

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0000049 - tRNA binding

References:

Protein features

PBO:0111751 - WD repeat protein

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0007021 - abolished tRNA cytosine 2'-O-methylation

References:

Genotypes:

FYPO:0007022 - abolished tRNA guanosine 2'-O-methylation

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0007024 - decreased tRNA guanosine 7-methylation

References:

Genotypes:

FYPO:0007025 - decreased tRNA guanosine N2-methylation

References:

Genotypes:

FYPO:0007026 - decreased tRNA wybutosine biosynthesis

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0007023 - increased tRNA guanosine 1-methylation

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0007018 - normal tRNA guanosine 2'-O-methylation

References:

Genotypes:

FYPO:0007019 - normal tRNA guanosine 7-methylation

References:

Genotypes:

FYPO:0007017 - normal tRNA pseudouridylation

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001214 - sensitive to potassium chloride

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000841 - sensitive to sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0001457 - sensitive to tunicamycin

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF00400WD40WD40_rptPFAM
PS50082WD_REPEATS_2WD40_rptPROSITE_PROFILES
PS50294WD_REPEATS_REGIONPROSITE_PROFILES
SM00320WD40_4WD40_rptSMART
SSF50978WD40 repeat-likeWD40_repeat_dom_sfSUPERFAMILY
G3DSA:2.130.10.10WD40/YVTN_repeat-like_dom_sfGENE3D
PTHR14344WD REPEAT PROTEINtRNA_Anticodon_Mtase-RegPANTHER

Orthologs

References / Literature

PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:29596413 - Lack of 2'-O-methylation in the tRNA anticodon loop of two phylogenetically distant yeast species activates the general amino acid control pathway.
Han L et al. PLoS Genet 2018 Mar;14(3):e1007288
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:25483073 - Chromosome segregation and organization are targets of 5'-Fluorouracil in eukaryotic cells.
Mojardín L et al. Cell Cycle 2015;14(2):206-18
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:25404562 - Conservation of an intricate circuit for crucial modifications of the tRNAPhe anticodon loop in eukaryotes.
Guy MP et al. RNA 2015 Jan;21(1):61-74
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:27918601 - Identifying genes required for respiratory growth of fission yeast.
Malecki M et al. Wellcome Open Res 2016;1:12