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protein coding gene - cdc23 (SPBC1347.10) - MCM-associated protein Mcm10

Gene summary

Standard name
cdc23
Systematic ID
SPBC1347.10
Product
MCM-associated protein Mcm10
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mcm10
UniProt ID
O42709
ORFeome ID
33/33D07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4079057..4081564 forward strand

Annotation

Comment

PBO:0018082 - mutants lacking primase activity fail to complement S. cerevisiae MCM10 deletion

References:

Complementation

PBO:0018081 - functionally complements S. cerevisiae MCM10

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Disease association

MONDO:0030266 - immunodeficiency 80 with or without congenital cardiomyopathy

References:

GO biological process

GO:0071515 - mating-type locus imprinting

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GO:1902315 - nuclear cell cycle DNA replication initiation

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GO cellular component

GO:0000785 - chromatin

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GO:0043596 - nuclear replication fork

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GO:0035861 - site of double-strand break

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GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

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GO:0003690 - double-stranded DNA binding

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GO:1904931 - MCM complex binding

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GO:0005515 - protein binding

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GO:0003697 - single-stranded DNA binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001427 - abnormal negative regulation of mitotic DNA replication initiation resulting in slow rereplication

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Genotypes:

FYPO:0000470 - decreased mating type switching

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0001974 - increased number of cells with 1C DNA content

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0001357 - normal vegetative cell population growth

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111871 - OB fold

Qualitative gene expression

PomGeneEx:0000016 - RNA level constant

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000611 - abnormal cell cycle arrest in mitotic S phase

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Genotypes:

FYPO:0000848 - abnormal chromosome morphology during vegetative growth

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Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

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Genotypes:

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

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Genotypes:

FYPO:0001093 - abolished chromatin binding

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Genotypes:

FYPO:0003996 - abolished DNA primase activity

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Genotypes:

FYPO:0005234 - abolished MCM complex binding

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Genotypes:

FYPO:0003548 - abolished mitotic G1/S phase transition following nitrogen starvation-induced G1 phase arrest

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Genotypes:

FYPO:0004392 - abolished positive regulation of DNA-directed DNA polymerase activity

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Genotypes:

FYPO:0004918 - abolished positive regulation of protein kinase activity

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Genotypes:

FYPO:0000705 - abolished protein-protein interaction

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Genotypes:

FYPO:0004386 - abolished single-stranded DNA binding

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0003352 - decreased DNA double-strand break formation at mating-type locus

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Genotypes:

FYPO:0003997 - decreased DNA primase activity

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Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0004387 - decreased positive regulation of DNA-directed DNA polymerase activity

References:

Genotypes:

FYPO:0002909 - decreased protein localization to chromatin during vegetative growth

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001982 - decreased rate of DNA replication during vegetative growth

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Genotypes:

FYPO:0004385 - decreased single-stranded DNA binding

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0005773 - elongated mononucleate aseptate vegetative cell

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Genotypes:

FYPO:0003998 - increased DNA primase activity

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Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

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Genotypes:

FYPO:0002420 - inviable after spore germination, single cell division, abnormal cell shape

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Genotypes:

FYPO:0002724 - inviable after spore germination, single or double cell division, elongated cell

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Genotypes:

FYPO:0002263 - inviable after spore germination, without cell division, cell cycle arrest, elongated cell

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Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0005233 - normal MCM complex binding

References:

Genotypes:

FYPO:0004921 - normal positive regulation of protein kinase activity

References:

Genotypes:

FYPO:0001509 - normal protein localization to chromatin during vegetative growth

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0004384 - normal single-stranded DNA binding

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Subunit composition

PBO:0015212 - homomeric(2)

References:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09329zf-primaseZnf_Mcm10/DnaGPfam
PF22379OB_MCM10OB_MCM10Pfam
PS52065MCM10_IDMCM10_IDPROSITE profiles
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
PTHR13454Mcm10PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polyampholytedisorder_predictionMobiDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMobiDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:9745018 - The essential schizosaccharomyces pombe cdc23 DNA replication gene shares structural and functional homology with the Saccharomyces cerevisiae DNA43 (MCM10) gene.
Aves SJ et al. Curr Genet 1998 Sep;34(3):164-71
PMID:15941470 - Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.
Yang X et al. BMC Mol Biol 2005 Jun 07;6:13
PMID:21307936 - Structural basis for the subunit assembly of the anaphase-promoting complex.
Schreiber A et al. Nature 2011 Feb 10;470(7333):227-32
PMID:22433840 - Mcm10 plays an essential role in origin DNA unwinding after loading of the CMG components.
Kanke M et al. EMBO J 2012 May 02;31(9):2182-94
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:9891047 - Mutator phenotype induced by aberrant replication.
Liu VF et al. Mol Cell Biol 1999 Feb;19(2):1126-35
PMID:17660542 - Mms22 preserves genomic integrity during DNA replication in Schizosaccharomyces pombe.
Dovey CL et al. Genetics 2007 Sep;177(1):47-61
PMID:14766746 - Primer utilization by DNA polymerase alpha-primase is influenced by its interaction with Mcm10p.
Fien K et al. J Biol Chem 2004 Apr 16;279(16):16144-53
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.
Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43
PMID:1934126 - Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.
Grallert B et al. Curr Genet 1991 Aug;20(3):199-204
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:17112379 - The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.
Locovei AM et al. Cell Div 2006 Nov 17;1:27
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:10388806 - Rereplication phenomenon in fission yeast requires MCM proteins and other S phase genes.
Snaith HA et al. Genetics 1999 Jul;152(3):839-51
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:11027263 - Schizosaccharomyces pombe Hsk1p is a potential cds1p target required for genome integrity.
Snaith HA et al. Mol Cell Biol 2000 Nov;20(21):7922-32
PMID:23583778 - The four canonical tpr subunits of human APC/C form related homo-dimeric structures and stack in parallel to form a TPR suprahelix.
Zhang Z et al. J Mol Biol 2013 Nov 15;425(22):4236-48
PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.
Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87
PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.
Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.
Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:12604790 - The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase.
Lee JK et al. Proc Natl Acad Sci U S A 2003 Mar 04;100(5):2334-9
PMID:21945095 - Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1).
Taylor M et al. DNA Repair (Amst) 2011 Nov 10;10(11):1154-63
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
GO_REF:0000002 - Comments
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.
Liang DT et al. Genetics 2001 Oct;159(2):471-86
PMID:16720577 - Fission yeast Mcm10p contains primase activity.
Fien K et al. J Biol Chem 2006 Aug 04;281(31):22248-22260
PMID:6943408 - Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe.
Nasmyth K et al. Mol Gen Genet 1981;182(1):119-24
PMID:12185500 - Fission yeast Cdc23 interactions with DNA replication initiation proteins.
Hart EA et al. Curr Genet 2002 Aug;41(5):342-8
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36