protein coding gene - med15 (SPBC146.01) - mediator complex subunit Med15

Gene summary

Standard name: med15
Systematic ID: SPBC146.01
Product: mediator complex subunit Med15
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: SPBP35G2.15, gal11
UniProt ID: Q9Y808
ORFeome ID: 30/30C02
Characterisation status: biological role published
Feature type: mRNA gene
Genomic location: chromosome II: 996683..1000175 forward strand

Annotation

GO biological process

GO:0045944 - positive regulation of transcription by RNA polymerase II

References:

GO_REF:0000024

GO cellular component

GO:0000785 - chromatin

References:

PMID:20622008

GO:0016592 - mediator complex

References:

GO_REF:0000024

GO:0005634 - nucleus

References:

PMID:16823372

GO molecular function

GO:0036033 - mediator complex binding

References:

PMID:20622008

GO:0003713 - transcription coactivator activity

References:

GO_REF:0000024

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

PMID:26537787

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

PMID:27984744

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0005521 - abnormal nucleosome organization

References:

PMID:20622008

Genotypes:

med15delta

FYPO:0004527 - abnormal regulation of transcription during vegetative growth

References:

PMID:20622008

Genotypes:

med15delta

FYPO:0000134 - branched, elongated, multiseptate cell

References:

PMID:15507118

Genotypes:

gal11-879(unknown)

FYPO:0002353 - decreased chromatin silencing

References:

PMID:20622008

Genotypes:

med15delta

FYPO:0000223 - elongated multiseptate vegetative cell

References:

PMID:15507118

Genotypes:

gal11-879(unknown)

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

PMID:23697806

Genotypes:

med15delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

med15delta

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

PMID:24929437

Protein features

ID	Name	InterPro name	DB name
PF05397	Med15_fungi	Mediator_Med15_fun	Pfam
PF16987	KIX_2	MED15_KIX	Pfam
cd12191	gal11_coact	Gal11_coact	CDD
G3DSA:1.10.246.20		KIX_dom_sf	CATH-Gene3D
G3DSA:1.10.287.2920			CATH-Gene3D
Coil			COILS
mobidb-lite-Disorder	disorder_prediction		MobiDB-Disorder
mobidb-lite-Low-complexity	disorder_prediction		MobiDB-Low-complexity
mobidb-lite-Polar	disorder_prediction		MobiDB-Polar

Orthologs

MED15 (HGNC:14248) Homo sapiens
med15 (SJAG_03981) Schizosaccharomyces japonicus
GAL11 (YOL051W) Saccharomyces cerevisiae

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.: Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:20622008 - A chromatin-remodeling protein is a component of fission yeast mediator.: Khorosjutina O et al. J Biol Chem 2010 Sep 24;285(39):29729-37
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.: Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.: Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.: Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.: Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:24929437 - The translational landscape of fission-yeast meiosis and sporulation.: Duncan CD et al. Nat Struct Mol Biol 2014 Jul;21(7):641-7
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.: Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:18257517 - Phosphoproteome analysis of fission yeast.: Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.: Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.: Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.: Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;