protein coding gene - prp2 (SPBC146.07) - U2 small nuclear RNA auxiliary factor small subunit, U2AF-59

Gene summary

Standard name

prp2

Systematic ID

SPBC146.07

Product

U2 small nuclear RNA auxiliary factor small subunit, U2AF-59

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

mis11, uaf1, ods1

UniProt ID

P36629

ORFeome ID

40/40F01

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 1013310..1015179 forward strand

Annotation

Protein features

Orthologs

• U2AF2 (HGNC:23156) Homo sapiens
• prp2 (SJAG_03995) Schizosaccharomyces japonicus
• MUD2 (YKL074C) Saccharomyces cerevisiae

References / Literature

PMID:17362205 - Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast.

Tang Z et al. Biochem J 2007 Jul 01;405(1):21-30

PMID:38833506 - A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast.

Borao S et al. PLoS Genet 2024 Jun 04;20(6):e1011316

PMID:24586893 - Wat1/pop3, a conserved WD repeat containing protein acts synergistically with checkpoint kinase Chk1 to maintain genome ploidy in fission yeast S. pombe.

Verma SK et al. PLoS One 2014;9(2):e89587

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:20018856 - Involvement of the spliceosomal U4 small nuclear RNA in heterochromatic gene silencing at fission yeast centromeres.

Chinen M et al. J Biol Chem 2010 Feb 19;285(8):5630-8

PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.

Ren L et al. PLoS One 2011 Feb 28;6(2):e16719

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.

Das J et al. Sci Signal 2013 May 21;6(276):ra38

PMID:8862522 - A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.

Lundgren K et al. Mol Biol Cell 1996 Jul;7(7):1083-94

PMID:14730023 - The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.

Banerjee H et al. RNA 2004 Feb;10(2):240-53

PMID:9671485 - A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.

Gozani O et al. Mol Cell Biol 1998 Aug;18(8):4752-60

PMID:26215567 - A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.

Yoshida H et al. Genes Dev 2015 Aug 01;29(15):1649-60

PMID:9917066 - Molecular genetic analysis of U2AF59 in Schizosaccharomyces pombe: differential sensitivity of introns to mutational inactivation.

Romfo CM et al. RNA 1999 Jan;5(1):49-65

PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.

Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671

PMID:12374752 - Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23).

Huang T et al. EMBO J 2002 Oct 15;21(20):5516-26

PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.

Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58

PMID:10923022 - Mutations in the large subunit of U2AF disrupt pre-mRNA splicing, cell cycle progression and nuclear structure.

Beales M et al. Yeast 2000 Aug;16(11):1001-13

PMID:34830325 - Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.

Borao S et al. Int J Mol Sci 2021 Nov 18;22(22)

PMID:9488736 - Fission yeast mitotic regulator Dsk1 is an SR protein-specific kinase.

Tang Z et al. J Biol Chem 1998 Mar 06;273(10):5963-9

PMID:2798130 - Splicing of the U6 RNA precursor is impaired in fission yeast pre-mRNA splicing mutants.

Potashkin J et al. Nucleic Acids Res 1989 Oct 11;17(19):7821-31

PMID:22683458 - Interacting factors and cellular localization of SR protein-specific kinase Dsk1.

Tang Z et al. Exp Cell Res 2012 Oct 01;318(16):2071-84

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:8211184 - U2AF homolog required for splicing in vivo.

Potashkin J et al. Science 1993 Oct 22;262(5133):573-5

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:17130122 - Mutations in the SF1-U2AF59-U2AF23 complex cause exon skipping in Schizosaccharomyces pombe.

Haraguchi N et al. J Biol Chem 2007 Jan 26;282(4):2221-8

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:34706246 - Cross talk between the upstream exon-intron junction and Prp2 facilitates splicing of non-consensus introns.

Hümmer S et al. Cell Rep 2021 Oct 26;37(4):109893

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:8657565 - The small subunit of the splicing factor U2AF is conserved in fission yeast.

Wentz-Hunter K et al. Nucleic Acids Res 1996 May 15;24(10):1849-54

PMID:22240020 - Characterization of the ptr5+ gene involved in nuclear mRNA export in fission yeast.

Watanabe N et al. Biochem Biophys Res Commun 2012 Feb 03;418(1):62-6

PMID:27875300 - The Fission Yeast Pre-mRNA-processing Factor 18 (prp18+) Has Intron-specific Splicing Functions with Links to G1-S Cell Cycle Progression.

Vijaykrishna N et al. J Biol Chem 2016 Dec 30;291(53):27387-27402

PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:2721492 - Pre-mRNA splicing mutants of Schizosaccharomyces pombe.

Potashkin J et al. EMBO J 1989 Feb;8(2):551-9

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:32958768 - Elucidation of the aberrant 3' splice site selection by cancer-associated mutations on the U2AF1.

Yoshida H et al. Nat Commun 2020 Sep 21;11(1):4744

PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.

Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31

PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.

Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86

PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.

Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701

PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.

Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:23754748 - Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly.

Banerjee S et al. Mol Cell Biol 2013 Aug;33(16):3125-36

PMID:9371883 - Molecular characterization of a novel fission yeast gene spUAP2 that interacts with the splicing factor spU2AF59.

McKinney R et al. Curr Genet 1997 Nov;32(5):323-30

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.

Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:26167880 - SR protein kinases promote splicing of nonconsensus introns.

Lipp JJ et al. Nat Struct Mol Biol 2015 Aug;22(8):611-7

PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.

Bayne EH et al. Science 2008 Oct 24;322(5901):602-6

PMID:11686295 - Conserved Wat1/Pop3 WD-repeat protein of fission yeast secures genome stability through microtubule integrity and may be involved in mRNA maturation.

Ochotorena IL et al. J Cell Sci 2001 Aug;114(Pt 16):2911-20

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:11350031 - Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes differential inhibition of pre-mRNA splicing and arrest of cell-cycle progression in Schizosaccharomyces pombe.

Habara Y et al. RNA 2001 May;7(5):671-81

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:10629038 - Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.

Tang Z et al. Mol Cell Biol 2000 Feb;20(3):816-24

PMID:11252721 - Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor.

Schwelnus W et al. EMBO Rep 2001 Jan;2(1):35-41

PMID:9837997 - The fission yeast prp10(+) gene involved in pre-mRNA splicing encodes a homologue of highly conserved splicing factor, SAP155.

Habara Y et al. Nucleic Acids Res 1998 Dec 15;26(24):5662-9

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:9003295 - Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces pombe.

Urushiyama S et al. Mol Gen Genet 1996 Nov 27;253(1-2):118-27

PMID:26302002 - Cwf16p Associating with the Nineteen Complex Ensures Ordered Exon Joining in Constitutive Pre-mRNA Splicing in Fission Yeast.

Sasaki-Haraguchi N et al. PLoS One 2015;10(8):e0136336

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36