protein coding gene - cut2 (SPBC14C8.01c) - sister chromatid separation inhibitor, securin

Gene summary

Standard name

cut2

Systematic ID

SPBC14C8.01c

Product

sister chromatid separation inhibitor, securin

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

SPBC1815.02c

UniProt ID

P21135

ORFeome ID

40/40B04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2203000..2204216 reverse strand

Annotation

Protein features

Orthologs

• PTTG3P (HGNC:13422) Homo sapiens
• PTTG1 (HGNC:9690) Homo sapiens
• PTTG2 (HGNC:9691) Homo sapiens
• cut2 (SJAG_00460) Schizosaccharomyces japonicus
• PDS1 (YDR113C) Saccharomyces cerevisiae

References / Literature

PMID:11460168 - A MAP kinase-dependent actin checkpoint ensures proper spindle orientation in fission yeast.

Gachet Y et al. Nature 2001 Jul 19;412(6844):352-5

PMID:11777938 - Polo boxes and Cut23 (Apc8) mediate an interaction between polo kinase and the anaphase-promoting complex for fission yeast mitosis.

May KM et al. J Cell Biol 2002 Jan 07;156(1):23-8

PMID:22645648 - The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.

Ikai N et al. Open Biol 2011 Nov;1(3):110007

PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.

Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7

PMID:12668659 - An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation.

Tasto JJ et al. J Cell Biol 2003 Mar 31;160(7):1093-103

PMID:11598020 - Establishment and maintenance of sister chromatid cohesion in fission yeast by a unique mechanism.

Tanaka K et al. EMBO J 2001 Oct 15;20(20):5779-90

PMID:16904908 - Cdc48 is required for the stability of Cut1/separase in mitotic anaphase.

Ikai N et al. J Struct Biol 2006 Oct;156(1):50-61

PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.

Hirano T et al. EMBO J 1986 Nov;5(11):2973-9

PMID:21389117 - Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast.

Kimata Y et al. Mol Biol Cell 2011 May;22(9):1486-94

PMID:9635190 - Cut1 is loaded onto the spindle by binding to Cut2 and promotes anaphase spindle movement upon Cut2 proteolysis.

Kumada K et al. Curr Biol 1998 May 21;8(11):633-41

PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.

Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82

PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.

Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834

PMID:11882285 - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.

Toyoda Y et al. Curr Biol 2002 Mar 05;12(5):347-58

PMID:22084378 - Nutrient limitations alter cell division control and chromosome segregation through growth-related kinases and phosphatases.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 2011 Dec 27;366(1584):3508-20

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:30072439 - The putative ceramide-conjugation protein Cwh43 regulates G0 quiescence, nutrient metabolism and lipid homeostasis in fission yeast.

Nakazawa N et al. J Cell Sci 2018 Aug 21;131(16)

PMID:17178839 - Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase.

Kearsey SE et al. Mol Cell Biol 2007 Mar;27(5):1558-67

PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.

Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397

PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.

Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71

GO_REF:0000002 - Comments

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

PMID:16483313 - Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase.

Nagao K et al. Genes Cells 2006 Mar;11(3):247-60

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.

Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838

PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38

PMID:29735656 - Suppressor mutation analysis combined with 3D modeling explains cohesin's capacity to hold and release DNA.

Xu X et al. Proc Natl Acad Sci U S A 2018 May 22;115(21):E4833-E4842

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:2203537 - The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene.

Uzawa S et al. Cell 1990 Sep 07;62(5):913-25

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:16861909 - Fission yeast MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses.

Kawasaki Y et al. Cell Cycle 2006 Aug;5(16):1831-9

PMID:9312055 - Fission yeast Cut2 required for anaphase has two destruction boxes.

Funabiki H et al. EMBO J 1997 Oct 01;16(19):5977-87

PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.

Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59

PMID:14532136 - Rec8 cleavage by separase is required for meiotic nuclear divisions in fission yeast.

Kitajima TS et al. EMBO J 2003 Oct 15;22(20):5643-53

PMID:35320724 - Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.

Vijayakumari D et al. Cell Rep 2022 Mar 22;38(12):110554

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:24583014 - Slow checkpoint activation kinetics as a safety device in anaphase.

Kamenz J et al. Curr Biol 2014 Mar 17;24(6):646-51

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:10651900 - Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin.

Yanagida M Genes Cells 2000 Jan;5(1):1-8

PMID:8395535 - Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis.

Samejima I et al. J Cell Sci 1993 May;105 ( Pt 1):135-43

PMID:12653962 - A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids.

Matsumura T et al. Genes Cells 2003 Apr;8(4):341-55

PMID:15791259 - Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p.

Izawa D et al. Nature 2005 Mar 24;434(7032):529-33

PMID:38780300 - Nitrogen availability is important for preventing catastrophic mitosis in fission yeast.

Zemlianski V et al. J Cell Sci 2024 May 23;

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:19117951 - Interaction of APC/C-E3 ligase with Swi6/HP1 and Clr4/Suv39 in heterochromatin assembly in fission yeast.

Dubey RN et al. J Biol Chem 2009 Mar 13;284(11):7165-76

PMID:8632802 - Cut2 proteolysis required for sister-chromatid seperation in fission yeast.

Funabiki H et al. Nature 1996 May 30;381(6581):438-41

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.

Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7

PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.

Mata J et al. Nat Genet 2002 Sep;32(1):143-7

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:8978688 - Fission yeast Cut1 and Cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes.

Funabiki H et al. EMBO J 1996 Dec 02;15(23):6617-28

PMID:11069892 - Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase.

Tomonaga T et al. Genes Dev 2000 Nov 01;14(21):2757-70

PMID:15329725 - Separase-mediated cleavage of cohesin at interphase is required for DNA repair.

Nagao K et al. Nature 2004 Aug 26;430(7003):1044-8

PMID:15161942 - The nucleolus is involved in mRNA export from the nucleus in fission yeast.

Ideue T et al. J Cell Sci 2004 Jun 15;117(Pt 14):2887-95

PMID:18331722 - A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast.

Kimata Y et al. Dev Cell 2008 Mar;14(3):446-54

PMID:1934126 - Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.

Grallert B et al. Curr Genet 1991 Aug;20(3):199-204

PMID:14511667 - A mutation in the gene involved in sister chromatid separation causes a defect in nuclear mRNA export in fission yeast.

Kalam Azad A et al. Biochem Biophys Res Commun 2003 Oct 10;310(1):176-81

PMID:39473973 - Limiting 20S proteasome assembly leads to unbalanced nucleo-cytoplasmic distribution of 26S/30S proteasomes and chronic proteotoxicity.

Ruiz-Romero G et al. iScience 2024 Nov 15;27(11):111095

PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119

PMID:12553909 - Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome.

Yen HC et al. Cell 2003 Jan 24;112(2):207-17