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protein coding gene - rpb8 (SPBC14C8.12) - DNA-directed RNA polymerase I, II and III subunit Rpb8

Gene summary

Standard name
rpb8
Systematic ID
SPBC14C8.12
Product
DNA-directed RNA polymerase I, II and III subunit Rpb8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q92399
ORFeome ID
31/31E02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2223646..2224720 forward strand

Annotation

GO biological process

GO:0006366 - transcription by RNA polymerase II

References:

GO:0006383 - transcription by RNA polymerase III

References:

GO:0006362 - transcription elongation by RNA polymerase I

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0005736 - RNA polymerase I complex

References:

GO:0005665 - RNA polymerase II, core complex

References:

GO:0016591 - RNA polymerase II, holoenzyme

References:

GO:0005666 - RNA polymerase III complex

References:

GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00048 - O4'-phospho-L-tyrosine

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Multi-locus phenotype

FYPO:0007786 - elongated cell with cell cycle arrest at meiotic G2/MI transition

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000021 - protein present

References:

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

References:

Genotypes:

FYPO:0002411 - inviable curved elongated vegetative cell

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03870RNA_pol_Rpb8RPABC3Pfam
SM00658RPOL8cRPABC3SMART
G3DSA:2.40.50.140:FF:000436CATH-FunFam
G3DSA:2.40.50.140NA-bd_OB-foldCATH-Gene3D
SSF50249NA-bd_OB-foldSUPERFAMILY
PTHR10917RPABC3PANTHER
PIRSF000779RNA_pol_Rpb8RPABC3PIRSF

Orthologs

References / Literature

PMID:9642054 - Two large subunits of the fission yeast RNA polymerase II provide platforms for the assembly of small subunits.
Ishiguro A et al. J Mol Biol 1998 Jun 19;279(4):703-12
PMID:15620689 - Glyceraldehyde-3-phosphate dehydrogenase and actin associate with RNA polymerase II and interact with its Rpb7 subunit.
Mitsuzawa H et al. FEBS Lett 2005 Jan 03;579(1):48-52
PMID:16204182 - RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing.
Djupedal I et al. Genes Dev 2005 Oct 01;19(19):2301-6
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15743411 - Studies of Schizosaccharomyces pombe TFIIE indicate conformational and functional changes in RNA polymerase II at transcription initiation.
Hayashi K et al. Genes Cells 2005 Mar;10(3):207-24
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:28476936 - Genome-wide screen for cell growth regulators in fission yeast.
Weston L et al. J Cell Sci 2017 Jun 15;130(12):2049-2055
PMID:33536435 - Conserved strategies of RNA polymerase I hibernation and activation.
Heiss FB et al. Nat Commun 2021 Feb 03;12(1):758
PMID:10648788 - Involvement of multiple subunit-subunit contacts in the assembly of RNA polymerase II.
Kimura M et al. Nucleic Acids Res 2000 Feb 15;28(4):952-9
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:10335407 - [Structural-functional characteristics of the Schizosaccharomyces pombe rpb8+ gene, coding the subunit of RNA polymerase I-III, specific only for eukaryotes].
Shpakovskiĭ GV et al. Bioorg Khim 1998 Feb;24(2):119-25
PMID:11839823 - Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.
Kimura M et al. Mol Cell Biol 2002 Mar;22(5):1577-88
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11168400 - Intracellular contents and assembly states of all 12 subunits of the RNA polymerase II in the fission yeast Schizosaccharomyces pombe.
Kimura M et al. Eur J Biochem 2001 Feb;268(3):612-9
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:32101745 - Dense Transposon Integration Reveals Essential Cleavage and Polyadenylation Factors Promote Heterochromatin Formation.
Lee SY et al. Cell Rep 2020 Feb 25;30(8):2686-2698.e8
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:39746995 - DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.
Kuś K et al. Nat Commun 2025 Jan 02;16(1):10
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:9325316 - RNA polymerase II subunits 2, 3, and 11 form a core subassembly with DNA binding activity.
Kimura M et al. J Biol Chem 1997 Oct 10;272(41):25851-5
PMID:9548938 - Location of subunit-subunit contact sites on RNA polymerase II subunit 3 from the fission yeast Schizosaccharomyces pombe.
Yasui K et al. Biochemistry 1998 Apr 21;37(16):5542-8
PMID:19458260 - Schizosacharomyces pombe RNA polymerase II at 3.6-A resolution.
Spåhr H et al. Proc Natl Acad Sci U S A 2009 Jun 09;106(23):9185-90
PMID:10523639 - The Rpb4 subunit of fission yeast Schizosaccharomyces pombe RNA polymerase II is essential for cell viability and similar in structure to the corresponding subunits of higher eukaryotes.
Sakurai H et al. Mol Cell Biol 1999 Nov;19(11):7511-8
PMID:9322754 - Gene organization and protein sequence of the small subunits of Schizosaccharomyces pombe RNA polymerase II.
Sakurai H et al. Gene 1997 Sep 01;196(1-2):165-74
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:11918671 - Level of the RNA polymerase II in the fission yeast stays constant but phosphorylation of its carboxyl terminal domain varies depending on the phase and rate of cell growth.
Sakurai H et al. Genes Cells 2002 Mar;7(3):273-84
PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.
Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201