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protein coding gene - ade8 (SPBC14F5.09c) - adenylosuccinate lyase Ade8

Gene summary

Standard name
ade8
Systematic ID
SPBC14F5.09c
Product
adenylosuccinate lyase Ade8
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O60105
ORFeome ID
40/40E09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4172822..4175500 reverse strand

Annotation

PBO:0004841 - 4.3.2.2

Disease association

MONDO:0007068 - adenylosuccinate lyase deficiency

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GO biological process

GO:0044208 - 'de novo' AMP biosynthetic process

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GO:0006189 - 'de novo' IMP biosynthetic process

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO molecular function

GO:0070626 - (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity

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GO:0004018 - N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000684 - decreased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0002829 - decreased cellular phytochelatin level

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000747 - growth auxotrophic for adenine

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Genotypes:

FYPO:0002243 - increased acid phosphatase activity

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009028 - increased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0000741 - increased cellular pigment accumulation

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Genotypes:

FYPO:0002728 - inviable swollen elongated cell with enlarged nucleus upon entry into stationary phase

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

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Genotypes:

FYPO:0001097 - sensitive to amitrole

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Genotypes:

FYPO:0001501 - sensitive to brefeldin A

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Genotypes:

FYPO:0000096 - sensitive to cadmium

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0001457 - sensitive to tunicamycin

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0003656 - sensitive to vanadate

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10397ADSL_CAdenyloSucc_lyase_CPFAM
PF00206Lyase_1Fumarate_lyase_NPFAM
cd03302Adenylsuccinate_lyase_2CDD
PS00163FUMARATE_LYASESFumarate_lyase_CSPROSITE_PATTERNS
SM00998ADSL_C_2AdenyloSucc_lyase_CSMART
PR00149FUMRATELYASEFumarate_lyase_famPRINTS
G3DSA:1.10.40.30:FF:000005FUNFAM
SSF48557L-aspartase-likeL-Aspartase-likeSUPERFAMILY
G3DSA:1.10.40.30GENE3D
G3DSA:1.10.275.60GENE3D
G3DSA:1.20.200.10Fumarase/aspartase (Central domain)GENE3D
PTHR43172ADENYLOSUCCINATE LYASEPANTHER
TIGR00928purBPur_lyaseNCBIFAM

Orthologs

References / Literature

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:8346915 - Two purine biosynthetic enzymes that are required for cadmium tolerance in Schizosaccharomyces pombe utilize cysteine sulfinate in vitro.
Juang RH et al. Arch Biochem Biophys 1993 Aug 01;304(2):392-401
PMID:36793083 - The SAGA histone acetyltransferase module targets SMC5/6 to specific genes.
Mahrik L et al. Epigenetics Chromatin 2023 Feb 16;16(1):6
PMID:1448066 - Purine biosynthetic genes are required for cadmium tolerance in Schizosaccharomyces pombe.
Speiser DM et al. Mol Cell Biol 1992 Dec;12(12):5301-10
PMID:499806 - Allelism of methionine-sensitive mutants of Schizosaccharomyces pombe to loci involved in adenine biosynthesis.
Strauss A Genet Res 1979 Jun;33(3):261-8
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:2743431 - An abnormal cell division cycle in an AIR carboxylase-deficient mutant of the fission yeast Schizosaccharomyces pombe.
Ishiguro J Curr Genet 1989 Jan;15(1):71-4
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.
Benko Z et al. Curr Genet 1997 Jun;31(6):481-7
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:1394510 - Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.
Schweingruber ME et al. Curr Genet 1992 Oct;22(4):289-92
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623