PomBase home

protein coding gene - tts1 (SPBC1539.04) - tetra spanning protein 1, Tts1

Gene summary

Standard name
tts1
Systematic ID
SPBC1539.04
Product
tetra spanning protein 1, Tts1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y7Z5
ORFeome ID
15/15D12
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4368342..4369816 forward strand

Annotation

GO biological process

GO:1990809 - endoplasmic reticulum tubular network membrane organization

References:

GO:0140480 - mitotic spindle pole body insertion into the nuclear envelope

References:

GO:1990608 - mitotic spindle pole body localization

References:

GO:0071763 - nuclear membrane organization

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0032541 - cortical endoplasmic reticulum

References:

GO:0005783 - endoplasmic reticulum

References:

GO:0071782 - endoplasmic reticulum tubular network

References:

GO:0005794 - Golgi apparatus

References:

GO:0005635 - nuclear envelope

References:

GO:0031965 - nuclear membrane

References:

GO molecular function

GO:0180020 - membrane bending activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

Multi-locus phenotype

FYPO:0000164 - abnormal cell separation after cytokinesis

References:

Genotypes:

FYPO:0000354 - abnormal endoplasmic reticulum morphology

References:

Genotypes:

FYPO:0003567 - abnormal mitotic spindle pole body insertion into nuclear envelope

References:

Genotypes:

FYPO:0006433 - abnormal tubular endoplasmic reticulum morphology

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

References:

Genotypes:

FYPO:0001371 - abolished protein localization to medial cortex

References:

Genotypes:

FYPO:0007268 - decreased cortical endoplasmic reticulum remodeling

References:

Genotypes:

FYPO:0006434 - decreased protein localization to medial cortical node, with protein distributed in cell cortex, during vegetative growth

References:

Genotypes:

FYPO:0000940 - decreased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0004648 - delayed onset of mitotic spindle assembly

References:

Genotypes:

FYPO:0007273 - increased cortical endoplasmic reticulum remodeling

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

References:

Genotypes:

FYPO:0003210 - mislocalized, misoriented septum

References:

Genotypes:

FYPO:0001390 - misoriented septum during vegetative growth

References:

Genotypes:

FYPO:0002018 - mitotic spindle absent from cell

References:

Genotypes:

FYPO:0000118 - multiseptate vegetative cell

References:

Genotypes:

FYPO:0002559 - normal protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0002558 - normal protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0002873 - normal septum orientation

References:

Genotypes:

FYPO:0006432 - normal vesicle-mediated transport during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000354 - abnormal endoplasmic reticulum morphology

References:

Genotypes:

FYPO:0000177 - abnormal mitotic spindle assembly

References:

Genotypes:

FYPO:0003783 - abnormal nuclear pore localization during mitosis

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0000251 - decreased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0003778 - decreased protein localization to endoplasmic reticulum tubular network

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003210 - mislocalized, misoriented septum

References:

Genotypes:

FYPO:0003781 - normal nuclear envelope morphology during mitotic interphase

References:

Genotypes:

FYPO:0003782 - normal nuclear pore localization during mitotic interphase

References:

Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0007448 - normal reticulophagy during nitrogen starvation

References:

Genotypes:

FYPO:0003788 - nuclear envelope protrusion present during mitosis

References:

Genotypes:

FYPO:0000763 - resistance to cadmium

References:

Genotypes:

FYPO:0000073 - resistance to caffeine

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0002578 - resistance to hydroxyurea

References:

Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0007933 - sensitive to 2,2′-dipyridyl

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0002328 - sensitive to terbinafine

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF03661TMEM33_Pom33TMEM33/Pom33Pfam
PTHR12703PER33/POM33_regulatorPANTHER
Transmembrane alpha helixDeepTMHMM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:24637836 - Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe.
Asakawa H et al. Nucleus 2014;5(2):149-62
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:27887640 - Functional and regulatory profiling of energy metabolism in fission yeast.
Malecki M et al. Genome Biol 2016 Nov 25;17(1):240
PMID:8873452 - Characterization of cwl1+, a gene from Schizosaccharomyces pombe whose overexpression causes cell lysis.
Godoy C et al. Yeast 1996 Aug;12(10):983-90
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:40124504 - Ltc1 localization by EMC regulates cell membrane fluidity to facilitate membrane protein biogenesis.
Berraquero M et al. iScience 2025 Mar 21;28(3):112096
PMID:39358553 - Ageing-associated long non-coding RNA extends lifespan and reduces translation in non-dividing cells.
Anver S et al. EMBO Rep 2024 Oct 02;
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:20434336 - The cortical ER network limits the permissive zone for actomyosin ring assembly.
Zhang D et al. Curr Biol 2010 Jun 08;20(11):1029-34
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:35609605 - Coordinated cortical ER remodeling facilitates actomyosin ring assembly.
Zhang D et al. Curr Biol 2022 Jun 20;32(12):2694-2703.e4
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:32023460 - Plasma Membrane Furrows Control Plasticity of ER-PM Contacts.
Ng AQE et al. Cell Rep 2020 Feb 04;30(5):1434-1446.e7
PMID:36408920 - UniProt: the Universal Protein Knowledgebase in 2023.
UniProt Consortium Nucleic Acids Res 2023 Jan 06;51(D1):D523-D531
PMID:37939137 - The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.
Zou CX et al. PLoS Biol 2023 Nov;21(11):e3002372
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.
Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25103238 - Tts1, the fission yeast homologue of the TMEM33 family, functions in NE remodeling during mitosis.
Zhang D et al. Mol Biol Cell 2014 Oct 01;25(19):2970-83
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:37191320 - Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein.
Fukuda T et al. Autophagy 2023 Oct;19(10):2657-2667
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:23041194 - Plasma membrane tethering of the cortical ER necessitates its finely reticulated architecture.
Zhang D et al. Curr Biol 2012 Nov 06;22(21):2048-52
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12