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protein coding gene - amo1 (SPBC15D4.10c) - nuclear rim protein Amo1

Gene summary

Standard name
amo1
Systematic ID
SPBC15D4.10c
Product
nuclear rim protein Amo1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74315
ORFeome ID
24/24A05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3028685..3030225 reverse strand

Annotation

GO biological process

GO:0140464 - subnuclear spatial organization of silent mating-type cassette heterochromatin

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GO cellular component

GO:0005737 - cytoplasm

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GO:0034399 - nuclear periphery

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GO:0005643 - nuclear pore

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GO:0005634 - nucleus

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GO:0044732 - mitotic spindle pole body

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GO molecular function

GO:0062239 - heterochromatin-nuclear membrane anchor activity

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GO:0046872 - metal ion binding

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Modification

MOD:00046 - O-phospho-L-serine

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Multi-locus phenotype

FYPO:0008368 - abolished histone H3-K9 trimethylation at centromere during vegetative growth

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Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0007478 - decreased epigenetic heterochromatin inheritance

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Genotypes:

FYPO:0005845 - decreased histone H3-K9 trimethylation at silent mating-type cassette during vegetative growth

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Genotypes:

FYPO:0002459 - viable branched, elongated vegetative cell

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Genotypes:

Protein features

PBO:0111777 - zf-CCCH type

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001584 - abnormal protein localization to cell tip during vegetative growth

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Genotypes:

FYPO:0000121 - abnormal sporulation

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Genotypes:

FYPO:0001394 - activation of monopolar cell growth at either end

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Genotypes:

FYPO:0003202 - actomyosin contractile ring contraction uncoupled from septum assembly

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Genotypes:

FYPO:0003327 - curved interphase microtubules

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Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

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Genotypes:

FYPO:0008367 - decreased duration of heterochromatin maintenance involved in chromatin silencing at silent mating type cassette

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Genotypes:

FYPO:0000883 - decreased histone H3-K9 trimethylation at centromere during vegetative growth

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Genotypes:

FYPO:0005845 - decreased histone H3-K9 trimethylation at silent mating-type cassette during vegetative growth

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Genotypes:

FYPO:0000708 - decreased mating efficiency

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Genotypes:

FYPO:0003306 - decreased mitotic index

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Genotypes:

FYPO:0003150 - decreased NETO

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Genotypes:

FYPO:0003208 - decreased protein localization to cell tip, with protein distributed in plasma membrane or cortex

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Genotypes:

FYPO:0003573 - decreased protein localization to heterochromatin at silent mating-type cassette

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

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Genotypes:

FYPO:0008369 - decreased silent mating type cassette heterochromatin tethering to nuclear periphery during vegetative growth

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Genotypes:

FYPO:0009095 - increased cell population growth on fructose carbon source

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Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

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Genotypes:

FYPO:0005995 - increased lncRNA level

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Genotypes:

FYPO:0003417 - increased microtubule dwell time at cell tip

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0005880 - long interphase microtubules curved around cell end

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0002818 - microtubule bundles present in decreased numbers

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Genotypes:

FYPO:0002071 - mislocalized nucleus during vegetative growth

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Genotypes:

FYPO:0000339 - mislocalized septum during vegetative growth

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Genotypes:

FYPO:0000771 - normal nuclear pore localization

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Genotypes:

FYPO:0004121 - normal protein import into nucleus during vegetative growth

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Genotypes:

FYPO:0005707 - normal protein localization to eMTOC

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Genotypes:

FYPO:0003185 - normal protein localization to microtubule cytoskeleton during mitotic interphase

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Genotypes:

FYPO:0002966 - normal protein localization to mitotic spindle

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Genotypes:

FYPO:0002967 - normal protein localization to mitotic spindle pole body

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Genotypes:

FYPO:0003193 - normal rate of microtubule depolymerization during vegetative growth

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Genotypes:

FYPO:0003225 - normal rate of microtubule polymerization during vegetative growth

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Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001884 - resistance to Calcofluor White

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Genotypes:

FYPO:0004025 - resistance to carbendazim

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Genotypes:

FYPO:0002766 - resistance to clotrimazole

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Genotypes:

FYPO:0002634 - resistance to cobalt

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0000069 - resistance to thiabendazole

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0002642 - sensitive to amphotericin B

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0001719 - sensitive to lithium

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Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

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Genotypes:

FYPO:0009082 - sensitive to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0002400 - single microtubule bundle during mitotic interphase

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0002215 - viable curved elongated vegetative cell

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF18044zf-CCCH_4Znf-CCCH_4Pfam
PF27634Amo1_NNUP42/Amo1_NPfam
PS50103ZF_C3H1Znf_CCCHPROSITE profiles
G3DSA:4.10.1000.10Znf_CCCH_sfCATH-Gene3D
PTHR46527Nucleoporin_NUP42PANTHER
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity
mobidb-lite-Polardisorder_predictionMobiDB-Polar

Orthologs

References / Literature

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.
Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:41501458 - Stress controls heterochromatin inheritance via histone H3 ubiquitylation.
Bhatt B et al. Nature 2026 Jan 07;
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:22806344 - Genome-wide screen reveals novel mechanisms for regulating cobalt uptake and detoxification in fission yeast.
Ryuko S et al. Mol Genet Genomics 2012 Aug;287(8):651-62
GO_REF:0000002 - Comments
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:32817556 - Integrity of a heterochromatic domain ensured by its boundary elements.
Charlton SJ et al. Proc Natl Acad Sci U S A 2020 Sep 01;117(35):21504-21511
PMID:24637836 - Characterization of nuclear pore complex components in fission yeast Schizosaccharomyces pombe.
Asakawa H et al. Nucleus 2014;5(2):149-62
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:34984977 - Functional profiling of long intergenic non-coding RNAs in fission yeast.
Rodriguez-Lopez M et al. Elife 2022 Jan 05;11
PMID:33176152 - Acute Heat Stress Leads to Reversible Aggregation of Nuclear Proteins into Nucleolar Rings in Fission Yeast.
Gallardo P et al. Cell Rep 2020 Nov 10;33(6):108377
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:22252817 - A genomewide screen in Schizosaccharomyces pombe for genes affecting the sensitivity of antifungal drugs that target ergosterol biosynthesis.
Fang Y et al. Antimicrob Agents Chemother 2012 Apr;56(4):1949-59
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:15797925 - The nuclear rim protein Amo1 is required for proper microtubule cytoskeleton organisation in fission yeast.
Pardo M et al. J Cell Sci 2005 Apr 15;118(Pt 8):1705-14
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:21340088 - Microarray-based target identification using drug hypersensitive fission yeast expressing ORFeome.
Arita Y et al. Mol Biosyst 2011 May;7(5):1463-72