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protein coding gene - uba1 (SPBC1604.21c) - ubiquitin activating enzyme E1 Uba1

Gene summary

Standard name
uba1
Systematic ID
SPBC1604.21c
Product
ubiquitin activating enzyme E1 Uba1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
ptr3, SPBC211.09
UniProt ID
O94609
ORFeome ID
48/48A09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3888677..3892036 forward strand

Annotation

Disease association

MONDO:0010532 - infantile-onset X-linked spinal muscular atrophy

References:

MONDO:0026777 - VEXAS syndrome

References:

GO biological process

GO:0006974 - DNA damage response

References:

GO:0006511 - ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005524 - ATP binding

References:

GO:0000287 - magnesium ion binding

References:

GO:0005515 - protein binding

References:

GO:0004839 - ubiquitin activating enzyme activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01148 - ubiquitinylated lysine

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000912 - abolished protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001913 - abolished ubiquitin ligase activity

References:

Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0008051 - decreased ubiquitin ligase activity

References:

Genotypes:

FYPO:0003119 - increased nuclear polyadenylated mRNA level during vegetative growth

References:

Genotypes:

FYPO:0002082 - increased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0002151 - inviable spore

References:

Genotypes:

FYPO:0001489 - inviable vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0003375 - normal ubiquinone binding

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF09358E1_UFDUb-activating_enz_E1_CPFAM
PF16191E1_4HBE1_4HBPFAM
PF10585UBA_E1_SCCHUBA_E1_SCCHPFAM
PF16190E1_FCCHE1_FCCHPFAM
PF00899ThiFThiF_NAD_FAD-bdPFAM
cd01490Ube1_repeat2CDD
cd01491Ube1_repeat1CDD
PS00865UBIQUITIN_ACTIVAT_2UBQ-activ_enz_E1_Cys_ASPROSITE_PATTERNS
SM00985UBA_e1_C_a_2Ub-activating_enz_E1_CSMART
PR01849UBIQUITINACTUBQ/SUMO-activ_enz_E1-likePRINTS
G3DSA:1.10.10.2660:FF:000001FUNFAM
G3DSA:3.50.50.80:FF:000001FUNFAM
G3DSA:2.40.30.180:FF:000001FUNFAM
G3DSA:3.10.290.60:FF:000002FUNFAM
G3DSA:3.40.50.720:FF:000015FUNFAM
G3DSA:3.40.50.12550:FF:000001FUNFAM
SSF69572Activating enzymes of the ubiquitin-like proteinsUbiquitin-activating_enzSUPERFAMILY
G3DSA:3.50.50.80Ub-E1_IAD_1GENE3D
G3DSA:3.40.50.720GENE3D
G3DSA:2.40.30.180E1_FCCH_sfGENE3D
G3DSA:3.10.290.60UBA_E1_C_sfGENE3D
G3DSA:1.10.10.2660Ubi_acti_E1_SCCHGENE3D
G3DSA:3.40.50.12550GENE3D
PTHR10953UBIQUITIN-ACTIVATING ENZYME E1ThiF/MoeB/HesAPANTHER
TIGR01408Ube1UBQ-activ_enz_E1NCBIFAM

Orthologs

References / Literature

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:25165823 - Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
Tsutsui Y et al. PLoS Genet 2014 Aug;10(8):e1004542
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:31397327 - Crystal structure of the Schizosaccharomyces pombe U7BR E2-binding region in complex with Ubc7.
Hann ZS et al. Acta Crystallogr F Struct Biol Commun 2019 Aug 01;75(Pt 8):552-560
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.
Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:35011726 - Role of Nse1 Subunit of SMC5/6 Complex as a Ubiquitin Ligase.
Kolesar P et al. Cells 2022 Jan 04;11(1)
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28162934 - S. pombe Uba1-Ubc15 Structure Reveals a Novel Regulatory Mechanism of Ubiquitin E2 Activity.
Lv Z et al. Mol Cell 2017 Feb 16;65(4):699-714.e6
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:31235585 - Structural basis for adenylation and thioester bond formation in the ubiquitin E1.
Hann ZS et al. Proc Natl Acad Sci U S A 2019 Jul 30;116(31):15475-15484
PMID:9168469 - Isolation and molecular characterization of mRNA transport mutants in Schizosaccharomyces pombe.
Azad AK et al. Mol Biol Cell 1997 May;8(5):825-41
PMID:28572513 - Domain alternation and active site remodeling are conserved structural features of ubiquitin E1.
Lv Z et al. J Biol Chem 2017 Jul 21;292(29):12089-12099
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:22274912 - Identification and mechanistic studies of a novel ubiquitin E1 inhibitor.
Ungermannova D et al. J Biomol Screen 2012 Apr;17(4):421-34
PMID:23416107 - Structure of a ubiquitin E1-E2 complex: insights to E1-E2 thioester transfer.
Olsen SK et al. Mol Cell 2013 Mar 07;49(5):884-96
PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.
Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834
PMID:39143218 - Structural basis for transthiolation intermediates in the ubiquitin pathway.
Kochańczyk T et al. Nature 2024 Sep;633(8028):216-223
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:25918164 - Endoplasmic Reticulum Exit of Golgi-resident Defective for SREBP Cleavage (Dsc) E3 Ligase Complex Requires Its Activity.
Raychaudhuri S et al. J Biol Chem 2015 Jun 05;290(23):14430-40
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:28552615 - SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.
Wei Y et al. Mol Cell 2017 Jun 01;66(5):581-596.e6