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protein coding gene - cha4 (SPBC1683.13c) - DNA-binding transcription factor, amino acid sensing, Cha4

Gene summary

Standard name
cha4
Systematic ID
SPBC1683.13c
Product
DNA-binding transcription factor, amino acid sensing, Cha4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P6I9
ORFeome ID
27/27C10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 170167..172266 reverse strand

Annotation

GO biological process

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO:0060261 - positive regulation of transcription initiation by RNA polymerase II

References:

GO:0006808 - regulation of nitrogen utilization

References:

GO cellular component

GO:0005634 - nucleus

References:

GO molecular function

GO:0000981 - DNA-binding transcription factor activity, RNA polymerase II-specific

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

GO:0008270 - zinc ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

Protein features

PBO:0111744 - zf-fungal Zn(2)-Cys(6) binuclear cluster domain

PBO:0111743 - zinc finger protein

Qualitative gene expression

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006549 - decreased gene expression

References:

Genotypes:

FYPO:0005014 - decreased histone H4-K12 acetylation during vegetative growth

References:

Genotypes:

FYPO:0000636 - increased cell population growth rate

References:

Genotypes:

FYPO:0006548 - increased gene expression

References:

Genotypes:

FYPO:0007631 - increased histone H4-K12 acetylation during vegetative growth

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Genotypes:

FYPO:0007506 - increased protein localization to chromatin at promoter

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Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0000243 - normal growth on proline nitrogen source

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000077 - resistance to rapamycin

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

Protein features

IDNameInterPro nameDB name
PF00172Zn_clusZn2Cys6_DnaBDPFAM
PF04082Fungal_transXnlR_reg_domPFAM
cd12148fungal_TF_MHRCDD
cd00067GAL4Zn2Cys6_DnaBDCDD
PS00463ZN2_CY6_FUNGAL_1Zn2Cys6_DnaBDPROSITE_PATTERNS
PS50048ZN2_CY6_FUNGAL_2Zn2Cys6_DnaBDPROSITE_PROFILES
SM00906Fungal_trans_2XnlR_reg_domSMART
SM00066gal4_2Zn2Cys6_DnaBDSMART
G3DSA:4.10.240.10:FF:000018FUNFAM
SSF57701Zn2/Cys6 DNA-binding domainZn2-C6_fun-type_DNA-bd_sfSUPERFAMILY
G3DSA:4.10.240.10Zn2-C6_fun-type_DNA-bd_sfGENE3D
PTHR31313TY1 ENHANCER ACTIVATORTranscr_Regulatory_ElemPANTHER

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:20404084 - Transcriptional activation of the general amino acid permease gene per1 by the histone deacetylase Clr6 Is regulated by Oca2 kinase.
Kaufmann I et al. Mol Cell Biol 2010 Jul;30(13):3396-410
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000002 - Comments
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.
Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053