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protein coding gene - spn3 (SPBC16A3.01) - mitotic septin Spn3

Gene summary

Standard name
spn3
Systematic ID
SPBC16A3.01
Product
mitotic septin Spn3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBC543.01c
UniProt ID
P48008
ORFeome ID
33/33B05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4298727..4300497 reverse strand

Annotation

GO biological process

GO:0000281 - mitotic cytokinesis

References:

GO:0000921 - septin ring assembly

References:

GO cellular component

GO:0032153 - cell division site

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0036391 - medial cortex septin ring

References:

GO:0015630 - microtubule cytoskeleton

References:

GO:0120104 - mitotic actomyosin contractile ring, proximal layer

References:

GO:0032151 - mitotic septin complex

References:

GO molecular function

GO:0005525 - GTP binding

References:

GO:0003924 - GTPase activity

References:

GO:0060090 - molecular adaptor activity

References:

GO:0005515 - protein binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

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Multi-locus phenotype

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0006062 - ectopic septin ring assembly during vegetative growth

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0001972 - abnormal cell separation after cytokinesis resulting in septated cell

References:

Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0002924 - decreased cell population growth on maltose carbon source

References:

Genotypes:

FYPO:0002557 - decreased protein localization to medial cortex during vegetative growth

References:

Genotypes:

FYPO:0006772 - dispersed actin cortical patch localization during mating

References:

Genotypes:

FYPO:0006062 - ectopic septin ring assembly during vegetative growth

References:

Genotypes:

FYPO:0009101 - increased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009093 - increased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0009098 - increased cell population growth on mannitol carbon source

References:

Genotypes:

FYPO:0000650 - increased septation index

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0004295 - multiseptate cell

References:

Genotypes:

FYPO:0004807 - narrow cell fusion site following mating

References:

Genotypes:

FYPO:0003334 - normal protein localization to septin ring

References:

Genotypes:

FYPO:0000590 - normal sporulation

References:

Genotypes:

FYPO:0009066 - resistance to amorolfine

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

References:

Genotypes:

FYPO:0002693 - resistance to diamide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0009070 - resistance to itraconazole

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Genotypes:

FYPO:0001583 - resistance to lithium

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0003848 - sensitive to hydrazinocurcumin

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009090 - sensitive to sodium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0001510 - viable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

Protein features

IDNameInterPro nameDB name
PF00735SeptinG_SEPTIN_domPFAM
cd01850CDC_SeptinSeptinCDD
PS51719G_SEPTING_SEPTIN_domPROSITE_PROFILES
G3DSA:3.40.50.300:FF:000162FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
PTHR18884SEPTINPANTHER
PIRSF006698SeptinSeptinPIRSF
CoilCoilCOILS

Orthologs

References / Literature

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18309270 - Genome-wide drug-induced haploinsufficient screening of fission yeast for identification of hydrazinocurcumin targets.
Baek ST et al. J Microbiol Biotechnol 2008 Feb;18(2):263-9
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:25724972 - Rho4 interaction with exocyst and septins regulates cell separation in fission yeast.
Pérez P et al. Microbiology (Reading) 2015 May;161(Pt 5):948-959
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:16291723 - Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p.
Morrell-Falvey JL et al. J Cell Sci 2005 Dec 01;118(Pt 23):5563-73
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:20123972 - Role of septins in the orientation of forespore membrane extension during sporulation in fission yeast.
Onishi M et al. Mol Cell Biol 2010 Apr;30(8):2057-74
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23956092 - [Cnb1 involved in cytokinesis in Schizosaccharomyces pombe].
Fan JQ et al. Yi Chuan 2013 Aug;35(8):1030-9
PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.
Jin QW et al. Genetics 2006 Apr;172(4):2101-12
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:18256290 - Schizosaccharomyces pombe Pxl1 is a paxillin homologue that modulates Rho1 activity and participates in cytokinesis.
Pinar M et al. Mol Biol Cell 2008 Apr;19(4):1727-38
PMID:24957674 - Yeast X-chromosome-associated protein 5 (Xap5) functions with H2A.Z to suppress aberrant transcripts.
Anver S et al. EMBO Rep 2014 Aug;15(8):894-902
GO_REF:0000002 - Comments
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:41171630 - Septins function in exocytosis via physical interactions with the exocyst complex in fission yeast cytokinesis.
Singh D et al. Elife 2025 Oct 31;13
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:25015293 - Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
Lei B et al. J Cell Sci 2014 Sep 15;127(Pt 18):4024-36
PMID:28914606 - Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.
McDonald NA et al. Elife 2017 Sep 15;6
PMID:15385632 - Requirements of fission yeast septins for complex formation, localization, and function.
An H et al. Mol Biol Cell 2004 Dec;15(12):5551-64
PMID:22905165 - The integrity of the cytokinesis machinery under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p.
Sharifmoghadam MR et al. PLoS One 2012;7(8):e42726
PMID:25411334 - The Rho-GEF Gef3 interacts with the septin complex and activates the GTPase Rho4 during fission yeast cytokinesis.
Wang N et al. Mol Biol Cell 2015 Jan 15;26(2):238-55
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:16317047 - Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression.
Petit CS et al. J Cell Sci 2005 Dec 15;118(Pt 24):5731-42