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protein coding gene - nrm1 (SPBC16A3.07c) - MBF complex corepressor Nrm1

Gene summary

Standard name
nrm1
Systematic ID
SPBC16A3.07c
Product
MBF complex corepressor Nrm1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O42913
ORFeome ID
15/15E09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4285944..4288583 forward strand

Annotation

GO biological process

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO:0006357 - regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0005829 - cytosol

References:

GO:0030907 - MBF transcription complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

References:

GO:0003714 - transcription corepressor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0000029 - abnormal chromosome segregation

References:

Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0000216 - abnormal negative regulation of mitotic DNA replication initiation

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Genotypes:

FYPO:0004516 - decreased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0005314 - decreased protein localization to chromatin at MCB promoters during vegetative growth

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000453 - DNA content decreased during vegetative growth

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Genotypes:

FYPO:0003343 - elongated multinucleate multiseptate cell, single septa between nuclei

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0003776 - increased pseudohyphal growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000047 - normal cell population growth

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

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Genotypes:

FYPO:0006742 - normal transcription from MCB promoter

References:

Genotypes:

FYPO:0003503 - normal vegetative cell length

References:

Genotypes:

FYPO:0004325 - sensitive to 5-fluorouracil

References:

Genotypes:

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

Qualitative gene expression

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000029 - abnormal chromosome segregation

References:

Genotypes:

FYPO:0000031 - abnormal mating

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0004988 - abnormal RNA level oscillation during mitotic cell cycle

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Genotypes:

FYPO:0006349 - abolished protein localization to chromatin at promoter

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

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Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0003743 - decreased cell population growth during glucose starvation

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Genotypes:

FYPO:0009078 - decreased cell population growth on ethanol carbon source

References:

Genotypes:

FYPO:0009053 - decreased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

References:

Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0005314 - decreased protein localization to chromatin at MCB promoters during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001253 - elongated multinucleate multiseptate vegetative cell, single septa between nuclei

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0003970 - incomplete mitotic sister chromatid segregation, with chromatin bridge

References:

Genotypes:

FYPO:0005258 - increased cell population growth at high temperature

References:

Genotypes:

FYPO:0003938 - increased cell population growth during glucose starvation

References:

Genotypes:

FYPO:0005261 - increased cell population growth on galactose carbon source

References:

Genotypes:

FYPO:0004167 - increased cell population growth on glycerol carbon source

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Genotypes:

FYPO:0009094 - increased cell population growth on lysine and proline nitrogen source

References:

Genotypes:

FYPO:0009074 - increased cell population growth on serine nitrogen source

References:

Genotypes:

FYPO:0009096 - increased cell population growth on xylose carbon source

References:

Genotypes:

FYPO:0001707 - increased mitotic DNA damage checkpoint activation

References:

Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0005026 - increased protein localization to chromatin at chromosome arms

References:

Genotypes:

FYPO:0006740 - increased protein localization to chromatin at MCB promoters during vegetative growth

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0000239 - increased transcription from MCB promoter

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

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Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

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Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0007553 - normal G1 to G0 transition

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Genotypes:

FYPO:0000964 - normal growth on thiabendazole

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Genotypes:

FYPO:0002843 - protein mislocalized to nucleoplasm

References:

Genotypes:

FYPO:0001986 - resistance to 5-fluorouracil

References:

Genotypes:

FYPO:0009066 - resistance to amorolfine

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

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Genotypes:

FYPO:0000763 - resistance to cadmium

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Genotypes:

FYPO:0001884 - resistance to Calcofluor White

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Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0002578 - resistance to hydroxyurea

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005969 - resistance to magnesium chloride

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Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0009039 - resistance to potassium chloride

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Genotypes:

FYPO:0009081 - resistance to potassium chloride and methyl methanesulfonate

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005968 - resistance to sodium chloride

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Genotypes:

FYPO:0009089 - resistance to sodium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0005266 - resistance to sodium dodecyl sulfate

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Genotypes:

FYPO:0009040 - resistance to tea tree oil

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0001034 - resistance to tunicamycin

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Genotypes:

FYPO:0007808 - resistance to valproic acid

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Genotypes:

FYPO:0001097 - sensitive to amitrole

References:

Genotypes:

FYPO:0007921 - sensitive to benzamidine

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0001701 - sensitive to bortezomib

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Genotypes:

FYPO:0000098 - sensitive to calcium

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009069 - sensitive to ciclopirox olamine

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Genotypes:

FYPO:0000102 - sensitive to cisplatin

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Genotypes:

FYPO:0001245 - sensitive to cobalt

References:

Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0003559 - sensitive to doxorubicin

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0007928 - sensitive to ethylenediaminetetraacetic acid

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000087 - sensitive to hydrogen peroxide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0009071 - sensitive to itraconazole

References:

Genotypes:

FYPO:0009086 - sensitive to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0000111 - sensitive to rapamycin

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0002701 - sensitive to torin1

References:

Genotypes:

FYPO:0002546 - sensitive to trichostatin A

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0003656 - sensitive to vanadate

References:

Genotypes:

FYPO:0009064 - sensitive to X-rays and rapamycin during vegetative growth.

References:

Genotypes:

FYPO:0009063 - sensitive to X-rays during vegetative growth

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF08528Whi5TF_Nrm1/Whi5PFAM
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte
mobidb-lite-Positive-Polyelectrolytedisorder_predictionMOBIDB-Positive-Polyelectrolyte

Orthologs

References / Literature

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:25669599 - Fitness profiling links topoisomerase II regulation of centromeric integrity to doxorubicin resistance in fission yeast.
Nguyen TT et al. Sci Rep 2015 Feb 11;5:8400
PMID:26791325 - Predicting chemotherapeutic drug combinations through gene network profiling.
Nguyen TT et al. Sci Rep 2016 Jan 21;6:18658
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:24006488 - The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor.
Ivanova T et al. Mol Biol Cell 2013 Nov;24(21):3350-7
PMID:31260531 - Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave.
González-Medina A et al. Nucleic Acids Res 2019 Sep 19;47(16):8439-8451
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:23950735 - Global analysis of fission yeast mating genes reveals new autophagy factors.
Sun LL et al. PLoS Genet 2013;9(8):e1003715
PMID:17936710 - Ctp1 is a cell-cycle-regulated protein that functions with Mre11 complex to control double-strand break repair by homologous recombination.
Limbo O et al. Mol Cell 2007 Oct 12;28(1):134-46
PMID:30635289 - Cell Cycle-Regulated Transcription of CENP-A by the MBF Complex Ensures Optimal Level of CENP-A for Centromere Formation.
Aristizabal-Corrales D et al. Genetics 2019 Mar;211(3):861-875
PMID:23365689 - Cellular robustness conferred by genetic crosstalk underlies resistance against chemotherapeutic drug doxorubicin in fission yeast.
Tay Z et al. PLoS One 2013;8(1):e55041
PMID:30154212 - Nutritional cell cycle reprogramming reveals that inhibition of Cdk1 is required for proper MBF-dependent transcription.
Rubio A et al. J Cell Sci 2018 Sep 20;131(18)
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28903048 - Set2 Methyltransferase Facilitates DNA Replication and Promotes Genotoxic Stress Responses through MBF-Dependent Transcription.
Pai CC et al. Cell Rep 2017 Sep 12;20(11):2693-2705
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:25701403 - Two fission yeast high mobility group box proteins in the maintenance of genomic integrity following doxorubicin insult.
Tang MY et al. Gene 2015 May 10;562(1):70-5
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37445861 - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK et al. Int J Mol Sci 2023 Jun 26;24(13)
PMID:18682565 - DNA replication checkpoint promotes G1-S transcription by inactivating the MBF repressor Nrm1.
de Bruin RA et al. Proc Natl Acad Sci U S A 2008 Aug 12;105(32):11230-5
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.
Li J et al. Nat Commun 2019 Mar 01;10(1):1002
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:40883509 - Nrm1 is a bistable switch connecting cell cycle progression to transcriptional control.
Murciano-Julià G et al. EMBO Rep 2025 Aug 29;
PMID:34296454 - The TOR-dependent phosphoproteome and regulation of cellular protein synthesis.
Mak T et al. EMBO J 2021 Aug 16;40(16):e107911
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:25533348 - Tolerance of deregulated G1/S transcription depends on critical G1/S regulon genes to prevent catastrophic genome instability.
Caetano C et al. Cell Rep 2014 Dec 24;9(6):2279-89
PMID:21359180 - Phosphorylation of the MBF repressor Yox1p by the DNA replication checkpoint keeps the G1/S cell-cycle transcriptional program active.
Caetano C et al. PLoS One 2011 Feb 16;6(2):e17211
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26890608 - A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave.
Gaspa L et al. Cell Cycle 2016;15(5):720-9
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:16916637 - Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback.
de Bruin RA et al. Mol Cell 2006 Aug;23(4):483-96
PMID:26891792 - Calcium modulation of doxorubicin cytotoxicity in yeast and human cells.
Nguyen TT et al. Genes Cells 2016 Mar;21(3):226-40
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6