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protein coding gene - nda2 (SPBC16A3.15c) - tubulin alpha 1

Gene summary

Standard name
nda2
Systematic ID
SPBC16A3.15c
Product
tubulin alpha 1
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
P04688
ORFeome ID
33/33A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4268912..4270761 forward strand

Annotation

Disease association

MONDO:0014531 - amyotrophic lateral sclerosis type 22

References:

MONDO:0979229 - congenital myopathy 26

References:

MONDO:0003441 - dystonic disorder

References:

MONDO:0054771 - keratoconus 9

References:

MONDO:0012703 - lissencephaly due to TUBA1A mutation

References:

MONDO:0018838 - lissencephaly spectrum disorders

References:

MONDO:0030827 - macrothrombocytopenia, isolated, 2, autosomal dominant

References:

MONDO:0005071 - nervous system disorder

References:

MONDO:0979231 - oocyte/zygote/embryo maturation arrest 23

References:

MONDO:0979232 - oocyte/zygote/embryo maturation arrest 24

References:

MONDO:0979230 - spastic ataxia 11, autosomal dominant

References:

MONDO:0100144 - Uner Tan Syndrome

References:

GO biological process

GO:0000226 - microtubule cytoskeleton organization

References:

GO:0000280 - nuclear division

References:

GO:0098863 - nuclear migration by microtubule mediated pushing forces

References:

GO cellular component

GO:0000235 - astral microtubule

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005525 - GTP binding

References:

GO:0003924 - GTPase activity

References:

GO:0005515 - protein binding

References:

GO:0005200 - structural constituent of cytoskeleton

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0001991 - inviable after spore germination, without cell division

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0002966 - normal protein localization to mitotic spindle

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000608 - abnormal cell cycle arrest in mitotic M phase

References:

Genotypes:

FYPO:0001352 - abnormal chromatin organization during vegetative growth

References:

Genotypes:

FYPO:0000051 - abnormal meiosis

References:

Genotypes:

FYPO:0000080 - decreased cell population growth at low temperature

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0001783 - elongated, constricted nucleus

References:

Genotypes:

FYPO:0000340 - haploinsufficient

References:

Genotypes:

FYPO:0000836 - increased protein level

References:

Genotypes:

FYPO:0000313 - inviable after spore germination, without cell division, with abnormal germ tube morphology

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002303 - inviable mononucleate monoseptate vegetative cell with anucleate compartment

References:

Genotypes:

FYPO:0000309 - inviable spore with abnormal morphology

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002071 - mislocalized nucleus during vegetative growth

References:

Genotypes:

FYPO:0006189 - normal protein localization to actin cable

References:

Genotypes:

FYPO:0002559 - normal protein localization to actomyosin contractile ring

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0003840 - sensitive to carbendazim

References:

Genotypes:

FYPO:0003021 - sensitive to EPC

References:

Genotypes:

FYPO:0001702 - sensitive to nocodazole

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF03953Tubulin_CTubulin/FtsZ_2-layer-sand-domPFAM
PF00091TubulinTubulin_FtsZ_GTPasePFAM
cd02186alpha_tubulinCDD
PS00227TUBULINTubulin_CSPROSITE_PATTERNS
SM00865Tubulin_C_4Tubulin/FtsZ_2-layer-sand-domSMART
SM00864Tubulin_4Tubulin_FtsZ_GTPaseSMART
PR01162ALPHATUBULINAlpha_tubulinPRINTS
PR01161TUBULINTubulinPRINTS
G3DSA:3.30.1330.20:FF:000001FUNFAM
G3DSA:1.10.287.600:FF:000005FUNFAM
G3DSA:3.40.50.1440:FF:000008FUNFAM
SSF55307Tubulin C-terminal domain-likeTub_FtsZ_CSUPERFAMILY
SSF52490Tubulin nucleotide-binding domain-likeTubulin/FtsZ_GTPase_sfSUPERFAMILY
G3DSA:3.40.50.1440Tubulin/FtsZ, GTPase domainTubulin/FtsZ_GTPase_sfGENE3D
G3DSA:3.30.1330.20Tubulin/FtsZ-like_CGENE3D
G3DSA:1.10.287.600Helix hairpin binTubulin_CGENE3D
PTHR11588TUBULINTubulinPANTHER
CoilCoilCOILS

Orthologs

References / Literature

PMID:20708587 - CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule.
Al-Bassam J et al. Dev Cell 2010 Aug 17;19(2):245-58
PMID:25002536 - A novel histone deacetylase complex in the control of transcription and genome stability.
Zilio N et al. Mol Cell Biol 2014 Sep 15;34(18):3500-14
PMID:27872152 - An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.
Matsuo Y et al. J Cell Sci 2016 Dec 15;129(24):4592-4606
PMID:11739790 - Two related kinesins, klp5+ and klp6+, foster microtubule disassembly and are required for meiosis in fission yeast.
West RR et al. Mol Biol Cell 2001 Dec;12(12):3919-32
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:10341216 - Sto1p, a fission yeast protein similar to tubulin folding cofactor E, plays an essential role in mitotic microtubule assembly.
Grishchuk EL et al. J Cell Sci 1999 Jun;112 ( Pt 12):1979-88
PMID:22696680 - Fission yeast Alp14 is a dose-dependent plus end-tracking microtubule polymerase.
Al-Bassam J et al. Mol Biol Cell 2012 Aug;23(15):2878-90
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:7334057 - Sequential alterations in the nuclear chromatin region during mitosis of the fission yeast Schizosaccharomyces pombe: video fluorescence microscopy of synchronously growing wild-type and cold-sensitive cdc mutants by using a DNA-binding fluorescent probe.
Toda T et al. J Cell Sci 1981 Dec;52:271-87
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:8887647 - Fission yeast mal2+ is required for chromosome segregation.
Fleig U et al. Mol Cell Biol 1996 Nov;16(11):6169-77
PMID:11082048 - Identification of an alpha-tubulin mutant of fission yeast from gamma-tubulin-interacting protein screening: genetic evidence for alpha-/gamma-tubulin interaction.
Takeoka A et al. J Cell Sci 2000 Dec;113 Pt 24:4557-62
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:9892665 - Moe1, a conserved protein in Schizosaccharomyces pombe, interacts with a Ras effector, Scd1, to affect proper spindle formation.
Chen CR et al. Proc Natl Acad Sci U S A 1999 Jan 19;96(2):517-22
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:11309419 - A mechanism for nuclear positioning in fission yeast based on microtubule pushing.
Tran PT et al. J Cell Biol 2001 Apr 16;153(2):397-411
PMID:34798057 - Transcription-wide mapping of dihydrouridine reveals that mRNA dihydrouridylation is required for meiotic chromosome segregation.
Finet O et al. Mol Cell 2022 Jan 20;82(2):404-419.e9
PMID:26864000 - Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.
Hussmann F et al. Sci Rep 2016 Feb 11;6:20653
PMID:28502666 - Purification and characterisation of the fission yeast Ndc80 complex.
Matsuo Y et al. Protein Expr Purif 2017 Jul;135:61-69
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11063680 - Fission yeast Ras1 effector Scd1 interacts with the spindle and affects its proper formation.
Li YC et al. Genetics 2000 Nov;156(3):995-1004
PMID:17452625 - Acetylation regulates tropomyosin function in the fission yeast Schizosaccharomyces pombe.
Skoumpla K et al. J Cell Sci 2007 May 01;120(Pt 9):1635-45
PMID:6887244 - Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe.
Toda T et al. J Mol Biol 1983 Aug 05;168(2):251-70
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:3785193 - Differential expressions of essential and nonessential alpha-tubulin genes in Schizosaccharomyces pombe.
Adachi Y et al. Mol Cell Biol 1986 Jun;6(6):2168-78
PMID:22500803 - EBs recognize a nucleotide-dependent structural cap at growing microtubule ends.
Maurer SP et al. Cell 2012 Apr 13;149(2):371-82
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:22540037 - Predicting the fission yeast protein interaction network.
Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67
PMID:24173154 - Visualization of chromosomes in mitotically arrested cells of the fission yeast Schizosaccharomyces pombe.
Umesono K et al. Curr Genet 1983 Apr;7(2):123-8
PB_REF:0000003 - Disease Association Curation
PMID:27834216 - Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism.
Britto M et al. Proc Natl Acad Sci U S A 2016 Nov 22;113(47):E7483-E7489
PMID:18600047 - Genome-wide identification of haploinsufficiency in fission yeast.
Baek ST et al. J Microbiol Biotechnol 2008 Jun;18(6):1059-63
PMID:22264709 - TUBA1A mutation-associated lissencephaly: case report and review of the literature.
Sohal AP et al. Pediatr Neurol 2012 Feb;46(2):127-31
GO_REF:0000051 - S. pombe keyword mapping
PMID:30659798 - Cryo-EM Structure (4.5-Å) of Yeast Kinesin-5-Microtubule Complex Reveals a Distinct Binding Footprint and Mechanism of Drug Resistance.
von Loeffelholz O et al. J Mol Biol 2019 Feb 15;431(4):864-872
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:9649518 - A screen for genes involved in the anaphase proteolytic pathway identifies tsm1(+), a novel Schizosaccharomyces pombe gene important for microtubule integrity.
Grishchuk EL et al. Genetics 1998 Jul;149(3):1251-64
PMID:6887245 - Cell division cycle genes nda2 and nda3 of the fission yeast Schizosaccharomyces pombe control microtubular organization and sensitivity to anti-mitotic benzimidazole compounds.
Umesono K et al. J Mol Biol 1983 Aug 05;168(2):271-84
PMID:9658169 - Identification of novel temperature-sensitive lethal alleles in essential beta-tubulin and nonessential alpha 2-tubulin genes as fission yeast polarity mutants.
Radcliffe P et al. Mol Biol Cell 1998 Jul;9(7):1757-71
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:9722643 - The fission yeast chromo domain encoding gene chp1(+) is required for chromosome segregation and shows a genetic interaction with alpha-tubulin.
Doe CL et al. Nucleic Acids Res 1998 Sep 15;26(18):4222-9
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:29235477 - Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.
von Loeffelholz O et al. Nat Commun 2017 Dec 13;8(1):2110
PMID:3409871 - Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis.
Ohkura H et al. EMBO J 1988 May;7(5):1465-73
PMID:24952478 - Recent advances in the genetics of dystonia.
Xiao J et al. Curr Neurol Neurosci Rep 2014 Aug;14(8):462
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:9348288 - Mal3, the fission yeast homologue of the human APC-interacting protein EB-1 is required for microtubule integrity and the maintenance of cell form.
Beinhauer JD et al. J Cell Biol 1997 Nov 03;139(3):717-28
PMID:18794845 - Mal3, the Schizosaccharomyces pombe homolog of EB1, changes the microtubule lattice.
des Georges A et al. Nat Struct Mol Biol 2008 Oct;15(10):1102-8
GO_REF:0000002 - Comments
PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.
Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35
PMID:6327053 - Identification of the pleiotropic cell division cycle gene NDA2 as one of two different alpha-tubulin genes in Schizosaccharomyces pombe.
Toda T et al. Cell 1984 May;37(1):233-42
PMID:10978278 - The cofactor-dependent pathways for alpha- and beta-tubulins in microtubule biogenesis are functionally different in fission yeast.
Radcliffe PA et al. Genetics 2000 Sep;156(1):93-103
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:8292390 - A novel mutation involved in the mitotic checkpoint in the fission yeast Schizosaccharomyces pombe.
Ishiguro J et al. Jpn J Genet 1993 Aug;68(4):265-76