protein coding gene - chp2 (SPBC16C6.10) - heterochromatin (HP1) family chromodomain protein Chp2

Gene summary

Standard name

chp2

Systematic ID

SPBC16C6.10

Product

heterochromatin (HP1) family chromodomain protein Chp2

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

clo2

UniProt ID

O42934

ORFeome ID

32/32F02

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 4349995..4351244 forward strand

Annotation

Protein features

Orthologs

• CBX1 (HGNC:1551) Homo sapiens
• CBX3 (HGNC:1553) Homo sapiens
• CBX5 (HGNC:1555) Homo sapiens
• chp2 (SJAG_01579) Schizosaccharomyces japonicus

References / Literature

PMID:15372076 - A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast.

Sadaie M et al. EMBO J 2004 Oct 01;23(19):3825-35

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:19136623 - Phosphorylation of Swi6/HP1 regulates transcriptional gene silencing at heterochromatin.

Shimada A et al. Genes Dev 2009 Jan 01;23(1):18-23

PMID:23604080 - HDAC-mediated suppression of histone turnover promotes epigenetic stability of heterochromatin.

Aygün O et al. Nat Struct Mol Biol 2013 May;20(5):547-54

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:14704433 - RNAi-mediated targeting of heterochromatin by the RITS complex.

Verdel A et al. Science 2004 Jan 30;303(5658):672-6

PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .

Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:30808655 - Transcriptional gene silencing requires dedicated interaction between HP1 protein Chp2 and chromatin remodeler Mit1.

Leopold K et al. Genes Dev 2019 May 01;33(9-10):565-577

PMID:31000521 - Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK in Schizosaccharomyces pombe .

Shen KF et al. Genetics 2019 Jun;212(2):417-430

PMID:20299449 - A chromodomain switch mediated by histone H3 Lys 4 acetylation regulates heterochromatin assembly.

Xhemalce B et al. Genes Dev 2010 Apr 01;24(7):647-52

PMID:18809570 - Balance between distinct HP1 family proteins controls heterochromatin assembly in fission yeast.

Sadaie M et al. Mol Cell Biol 2008 Dec;28(23):6973-88

PMID:27168121 - Discovery of genes involved in mitosis, cell division, cell wall integrity and chromosome segregation through construction of Schizosaccharomyces pombe deletion strains.

Chen JS et al. Yeast 2016 Sep;33(9):507-17

PMID:22727667 - Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatic gene silencing.

Ishida M et al. Mol Cell 2012 Jul 27;47(2):228-41

PMID:20421495 - RNAi and heterochromatin repress centromeric meiotic recombination.

Ellermeier C et al. Proc Natl Acad Sci U S A 2010 May 11;107(19):8701-5

PMID:25831549 - Epigenetics. Epigenetic inheritance uncoupled from sequence-specific recruitment.

Ragunathan K et al. Science 2015 Apr 03;348(6230):1258699

PMID:11129054 - A novel member of the Swi6p family of fission yeast chromo domain-containing proteins associates with the centromere in vivo and affects chromosome segregation.

Halverson D et al. Mol Gen Genet 2000 Nov;264(4):492-505

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:26438724 - H3K9 methylation extends across natural boundaries of heterochromatin in the absence of an HP1 protein.

Stunnenberg R et al. EMBO J 2015 Nov 12;34(22):2789-803

PMID:22474355 - Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly.

Hayashi A et al. Proc Natl Acad Sci U S A 2012 Apr 17;109(16):6159-64

PMID:27738016 - RNA interference is essential for cellular quiescence.

Roche B et al. Science 2016 Nov 11;354(6313)

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:26631744 - Conserved factor Dhp1/Rat1/Xrn2 triggers premature transcription termination and nucleates heterochromatin to promote gene silencing.

Chalamcharla VR et al. Proc Natl Acad Sci U S A 2015 Dec 22;112(51):15548-55

PMID:19111658 - HP1 proteins form distinct complexes and mediate heterochromatic gene silencing by nonoverlapping mechanisms.

Motamedi MR et al. Mol Cell 2008 Dec 26;32(6):778-90

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:30110338 - The binding of Chp2's chromodomain to methylated H3K9 is essential for Chp2's role in heterochromatin assembly in fission yeast.

Maksimov V et al. PLoS One 2018;13(8):e0201101

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:27648579 - A histone H3K9M mutation traps histone methyltransferase Clr4 to prevent heterochromatin spreading.

Shan CM et al. Elife 2016 Sep 20;5

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:39945308 - Characterization of the Swi6/HP1 binding motif in its partner protein reveals the basis for the functional divergence of the HP1 family proteins in fission yeast.

Oya T et al. FASEB J 2025 Feb 28;39(4):e70387

PMID:16246721 - The nucleation and maintenance of heterochromatin by a histone deacetylase in fission yeast.

Yamada T et al. Mol Cell 2005 Oct 28;20(2):173-85

PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.

Okita AK et al. Commun Biol 2019;2:17

PMID:31278118 - Cohesin Impedes Heterochromatin Assembly in Fission Yeast Cells Lacking Pds5.

Folco HD et al. Genetics 2019 Sep;213(1):127-141

PMID:37400983 - Cooperative DNA-binding activities of Chp2 are critical for its function in heterochromatin assembly.

Rahayu AF et al. J Biochem 2023 Sep 29;174(4):371-382

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:32269268 - Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin.

Dong W et al. Sci Rep 2020 Apr 08;10(1):6055

PMID:33378674 - The INO80 Complex Regulates Epigenetic Inheritance of Heterochromatin.

Shan CM et al. Cell Rep 2020 Dec 29;33(13):108561

PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.

Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:28942089 - Biochemical Basis for Distinct Roles of the Heterochromatin Proteins Swi6 and Chp2.

Isaac RS et al. J Mol Biol 2017 Nov 24;429(23):3666-3677

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24493644 - lncRNA recruits RNAi and the exosome to dynamically regulate pho1 expression in response to phosphate levels in fission yeast.

Shah S et al. Genes Dev 2014 Feb 01;28(3):231-44

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:24013500 - A conserved ncRNA-binding protein recruits silencing factors to heterochromatin through an RNAi-independent mechanism.

Marina DB et al. Genes Dev 2013 Sep 01;27(17):1851-6

PMID:24662054 - The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.

Creamer KM et al. Mol Cell Biol 2014 Jun;34(11):2046-61

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:10835380 - Four chromo-domain proteins of Schizosaccharomyces pombe differentially repress transcription at various chromosomal locations.

Thon G et al. Genetics 2000 Jun;155(2):551-68

PMID:15537537 - Heterochromatin regulates cell type-specific long-range chromatin interactions essential for directed recombination.

Jia S et al. Cell 2004 Nov 12;119(4):469-80

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:28682306 - Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription.

Jih G et al. Nature 2017 Jul 27;547(7664):463-467