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protein coding gene - dna2 (SPBC16D10.04c) - DNA replication endonuclease-helicase Dna2

Gene summary

Standard name
dna2
Systematic ID
SPBC16D10.04c
Product
DNA replication endonuclease-helicase Dna2
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9URU2
ORFeome ID
34/34B04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3592291..3596956 reverse strand

Annotation

Disease association

MONDO:0014062 - mitochondrial DNA deletion syndrome with progressive myopathy

References:

MONDO:0970950 - Rothmund-Thomson syndrome type 4

References:

MONDO:0014350 - Seckel syndrome 8

References:

GO biological process

GO:0000724 - double-strand break repair via homologous recombination

References:

GO:1903461 - Okazaki fragment processing involved in mitotic DNA replication

References:

GO:1903469 - removal of RNA primer involved in mitotic DNA replication

References:

GO:0031297 - replication fork processing

References:

GO:0071932 - replication fork reversal

References:

GO:0072429 - response to intra-S DNA damage checkpoint signaling

References:

GO:0000723 - telomere maintenance

References:

GO cellular component

GO:0140445 - chromosome, telomeric repeat region

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0042645 - mitochondrial nucleoid

References:

GO:0043596 - nuclear replication fork

References:

GO:0005654 - nucleoplasm

References:

GO:0005634 - nucleus

References:

GO:0035861 - site of double-strand break

References:

GO molecular function

GO:1990601 - 5' overhang single-stranded DNA endonuclease activity

References:

GO:0043139 - 5'-3' DNA helicase activity

References:

GO:0017108 - 5'-flap endonuclease activity

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GO:0016887 - ATP hydrolysis activity

References:

GO:0003682 - chromatin binding

References:

GO:0004518 - nuclease activity

References:

GO:0005515 - protein binding

References:

GO:0003723 - RNA binding

References:

GO:0017116 - single-stranded DNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00696 - phosphorylated residue

References:

Multi-locus phenotype

FYPO:0003067 - abnormal replication fork reversal

References:

Genotypes:

FYPO:0002907 - circularized chromosome during mitotic G1 phase

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0001514 - decreased protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002059 - inviable cell population

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000957 - normal growth on methyl methanesulfonate

References:

Genotypes:

FYPO:0000838 - normal protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0002239 - shortened telomeres during vegetative growth

References:

Genotypes:

FYPO:0005403 - telomeric 3' overhang absent during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000026 - ribosomal density increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0001343 - abnormal mitotic DNA replication

References:

Genotypes:

FYPO:0003067 - abnormal replication fork reversal

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0003071 - abolished single-stranded DNA 5'-3' exodeoxyribonuclease activity

References:

Genotypes:

FYPO:0000185 - decreased gene conversion during vegetative growth

References:

Genotypes:

FYPO:0004516 - decreased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0003068 - decreased protein localization to chromatin at replication forks during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0002474 - decreased protein localization to double-strand break site

References:

Genotypes:

FYPO:0003803 - decreased protein localization to telomere

References:

Genotypes:

FYPO:0005786 - decreased spatial extent of double-strand break processing

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0005452 - increased break-induced loss of heterozygosity

References:

Genotypes:

FYPO:0003584 - increased double-strand break repair via nonhomologous end joining

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0001038 - increased protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0002263 - inviable after spore germination, without cell division, cell cycle arrest, elongated cell

References:

Genotypes:

FYPO:0004255 - inviable elongated mononucleate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0004229 - normal growth during cellular response to ionizing radiation

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0003906 - normal growth on bleomycin

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0003069 - normal protein localization to chromatin at replication forks during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0002099 - normal protein phosphorylation during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0003107 - progressively shortening telomeres during vegetative growth

References:

Genotypes:

FYPO:0000095 - sensitive to bleomycin

References:

Genotypes:

FYPO:0000085 - sensitive to camptothecin

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

References:

PBO:0011067 - conserved in bacteria

References:

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF13087AAA_12DNA2/NAM7-like_CPFAM
PF13086AAA_11DNA2/NAM7_AAA_11PFAM
PF08696Dna2DNA_replication_fac_Dna2_NPFAM
cd18808SF1_C_Upf1SF1_C_Upf1CDD
cd22318DNA2_N-likeCDD
cd18041DEXXQc_DNA2Dna2/JHS1_DEXXQ-boxCDD
G3DSA:3.40.50.300:FF:000721FUNFAM
G3DSA:3.40.50.300:FF:000789FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:2.40.30.270GENE3D
G3DSA:3.90.320.10PDDEXK-like_dom_sfGENE3D
PTHR10887DNA2/NAM7 HELICASE FAMILYDNA2/NAM7-likePANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder

Orthologs

References / Literature

PMID:26465752 - Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.
Nanbu T et al. PLoS One 2015;10(10):e0140456
PMID:32784607 - The Chromatin Response to Double-Strand DNA Breaks and Their Repair.
Aleksandrov R et al. Cells 2020 Aug 07;9(8)
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:27729451 - Cdc24 Is Essential for Long-range End Resection in the Repair of Double-stranded DNA Breaks.
Zhang H et al. J Biol Chem 2016 Nov 25;291(48):24961-24973
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23188080 - The fission yeast MRN complex tethers dysfunctional telomeres for NHEJ repair.
Reis CC et al. EMBO J 2012 Dec 12;31(24):4576-86
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:15485922 - Fission yeast Dna2 is required for generation of the telomeric single-strand overhang.
Tomita K et al. Mol Cell Biol 2004 Nov;24(21):9557-67
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22682245 - The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing.
Hu J et al. Cell 2012 Jun 08;149(6):1221-32
PMID:23297348 - Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase.
Chen JS et al. Mol Cell Proteomics 2013 May;12(5):1074-86
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:10880469 - Genetic analyses of Schizosaccharomyces pombe dna2(+) reveal that dna2 plays an essential role in Okazaki fragment metabolism.
Kang HY et al. Genetics 2000 Jul;155(3):1055-67
PMID:32692737 - CRL4Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation.
Zhang JM et al. PLoS Genet 2020 Jul;16(7):e1008933
PMID:15915339 - Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair.
Tsutsui Y et al. Curr Genet 2005 Jul;48(1):34-43
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:18493607 - A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.
Natsume T et al. PLoS One 2008 May 21;3(5):e2221
PMID:26041456 - RPA prevents G-rich structure formation at lagging-strand telomeres to allow maintenance of chromosome ends.
Audry J et al. EMBO J 2015 Jul 14;34(14):1942-58
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:29084823 - Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast.
Navarro FJ et al. J Cell Sci 2017 Dec 15;130(24):4144-4154
PMID:23779158 - The proteasome factor Bag101 binds to Rad22 and suppresses homologous recombination.
Saito Y et al. Sci Rep 2013;3:2022
PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.
Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:28159842 - Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells.
Liu B et al. J Biol Chem 2017 Mar 24;292(12):4777-4788
PMID:25203555 - Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.
Zhang JM et al. PLoS Biol 2014 Sep;12(9):e1001946
PMID:9649516 - Fission yeast cdc24(+) encodes a novel replication factor required for chromosome integrity.
Gould KL et al. Genetics 1998 Jul;149(3):1221-33
PMID:15576681 - Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta.
Tanaka H et al. Nucleic Acids Res 2004;32(21):6367-77
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.
Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84
InterPro:IPR026851 - Unknown title
GO_REF:0000002 - Comments
PMID:12455694 - mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.
Williams DR et al. Eukaryot Cell 2002 Oct;1(5):758-73
PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.
Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43
PMID:15302919 - Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast.
Ryu GH et al. Nucleic Acids Res 2004;32(14):4205-16
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000036 - Manual annotations that require more than one source of functional data to support the assignment of the associated GO term
PMID:16040599 - Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast.
Kim J et al. Nucleic Acids Res 2005;33(13):4078-89
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7