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protein coding gene - mok13 (SPBC16D10.05) - spore wall alpha-1,3-glucan synthase Mok13

Gene summary

Standard name
mok13
Systematic ID
SPBC16D10.05
Product
spore wall alpha-1,3-glucan synthase Mok13
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9Y719
ORFeome ID
34/34B09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3597436..3604899 forward strand

Annotation

PBO:0002492 - 2.4.1.183

GO biological process

GO:0070591 - ascospore wall biogenesis

References:

GO:0070600 - fungal-type cell wall (1->3)-alpha-glucan biosynthetic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO:0005628 - prospore membrane

References:

GO molecular function

GO:0047657 - alpha-1,3-glucan synthase activity

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

SO:0001812 - transmembrane_helix

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000015 - RNA absent

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000175 - abnormal ascospore wall assembly

References:

Genotypes:

FYPO:0007493 - decreased ascospore wall alpha-glucan level

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0007494 - increased ascospore wall beta-glucan level

References:

Genotypes:

FYPO:0001309 - increased viability in stationary phase

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003795 - normal cell wall alpha-glucan level

References:

Genotypes:

FYPO:0003797 - normal conjugation frequency

References:

Genotypes:

FYPO:0002161 - normal growth on Calcofluor White

References:

Genotypes:

FYPO:0004892 - normal growth on echinocandin

References:

Genotypes:

FYPO:0001315 - normal vegetative cell morphology

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0009087 - resistance to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002767 - resistance to terbinafine

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0005132 - spores sensitive to cell wall-degrading enzymes

References:

Genotypes:

FYPO:0005131 - spores sensitive to ethanol

References:

Genotypes:

FYPO:0005130 - spores sensitive to heat

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

Protein features

IDNameInterPro nameDB name
PF26111Ig_Mok13Mok11-13/Ags1-like_IgPFAM
PF13692Glyco_trans_1_4PFAM
PF26775HTH_Mok13HTH_Mok13PFAM
PF26108GH_Mok13Mok11-13/Ags1-like_GHPFAM
PF00128Alpha-amylaseGH13_cat_domPFAM
PF2612712TM_Mok13Mok11-14/Ags1-like_TMPFAM
PF08323Glyco_transf_5Starch_synth_cat_domPFAM
PF26114Ig_2_Mok13Mok11-13/Ags1-like_Ig_2PFAM
PF26122CBM_Mok13Mok11-13/Ags1-like_CBMPFAM
cd11323AmyAc_AGSCDD
cd03791GT5_Glycogen_synthase_DULL1-likeCDD
SM00642aamyGH13_cat_domSMART
G3DSA:3.40.50.2000:FF:000052FUNFAM
G3DSA:3.20.20.80:FF:000073FUNFAM
G3DSA:3.40.50.2000:FF:000058FUNFAM
SSF51445(Trans)glycosidasesGH_hydrolase_sfSUPERFAMILY
SSF53756UDP-Glycosyltransferase/glycogen phosphorylaseSUPERFAMILY
G3DSA:3.40.50.2000Glycogen Phosphorylase B;GENE3D
G3DSA:3.20.20.80GlycosidasesGENE3D
PTHR47182CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATEDMok11-14/Ags1-likePANTHER
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:16420355 - Synthesis of alpha-glucans in fission yeast spores is carried out by three alpha-glucan synthase paralogues, Mok12p, Mok13p and Mok14p.
García I et al. Mol Microbiol 2006 Feb;59(3):836-53
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.
Liu NN et al. Cell Res 2010 Aug;20(8):963-5
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
GO_REF:0000002 - Comments
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.
Pan X et al. BMC Genomics 2012 Nov 23;13:662
PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.
Vega M et al. BMC Biol 2022 Jul 12;20(1):160
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:10087262 - Fission yeast alpha-glucan synthase Mok1 requires the actin cytoskeleton to localize the sites of growth and plays an essential role in cell morphogenesis downstream of protein kinase C function.
Katayama S et al. J Cell Biol 1999 Mar 22;144(6):1173-86
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:11152613 - Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes.
Krogh A et al. J Mol Biol 2001 Jan 19;305(3):567-80
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643