protein coding gene - pcn1 (SPBC16D10.09) - PCNA

Gene summary

Standard name

pcn1

Systematic ID

SPBC16D10.09

Product

PCNA

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

pcn

UniProt ID

Q03392

ORFeome ID

32/32A11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3616595..3617845 forward strand

Annotation

Protein features

Orthologs

• PCNA (HGNC:8729) Homo sapiens
• pcn1 (SJAG_04190) Schizosaccharomyces japonicus
• POL30 (YBR088C) Saccharomyces cerevisiae

References / Literature

PMID:17277362 - Brc1-mediated rescue of Smc5/6 deficiency: requirement for multiple nucleases and a novel Rad18 function.

Lee KM et al. Genetics 2007 Apr;175(4):1585-95

PMID:11805113 - Functional interaction of MutY homolog with proliferating cell nuclear antigen in fission yeast, Schizosaccharomyces pombe.

Chang DY et al. J Biol Chem 2002 Apr 05;277(14):11853-8

PMID:10698951 - Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21(Cip1)-like PCNA binding motif.

Reynolds N et al. EMBO J 2000 Mar 01;19(5):1108-18

PMID:21211723 - Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci.

Yamane K et al. Mol Cell 2011 Jan 07;41(1):56-66

PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:9326594 - DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits.

Zuo S et al. Proc Natl Acad Sci U S A 1997 Oct 14;94(21):11244-9

PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.

Dolan WP et al. Genetics 2010 May;185(1):39-53

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:15533944 - Interaction of checkpoint proteins Hus1/Rad1/Rad9 with DNA base excision repair enzyme MutY homolog in fission yeast, Schizosaccharomyces pombe.

Chang DY et al. J Biol Chem 2005 Jan 07;280(1):408-17

PMID:9592143 - Single point mutations located outside the inter-monomer domains abolish trimerization of Schizosaccharomyces pombe PCNA.

Piard K et al. Nucleic Acids Res 1998 Jun 01;26(11):2598-605

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:8730105 - Heterologous expression of the human cyclin-dependent kinase inhibitor p21Cip1 in the fission yeast, Schizosaccharomyces pombe reveals a role for PCNA in the chk1+ cell cycle checkpoint pathway.

Tournier S et al. Mol Biol Cell 1996 Apr;7(4):651-62

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:9563836 - Mutant PCNA alleles are associated with cdc phenotypes and sensitivity to DNA damage in fission yeast.

Arroyo MP et al. Mol Gen Genet 1998 Mar;257(5):505-18

PMID:19416828 - Schizosaccharomyces pombe Rtf2 mediates site-specific replication termination by inhibiting replication restart.

Inagawa T et al. Proc Natl Acad Sci U S A 2009 May 12;106(19):7927-32

PMID:10704216 - In vitro reconstitution of the Schizosaccharomyces pombe alternative excision repair pathway.

Alleva JL et al. Biochemistry 2000 Mar 14;39(10):2659-66

PMID:25293972 - Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).

Mastro TL et al. Genetics 2014 Dec;198(4):1457-72

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:15579205 - Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27.

Gray FC et al. BMC Mol Biol 2004 Dec 03;5(1):21

PMID:9928931 - The S/M checkpoint at 37 degrees C and the recovery of viability of the mutant poldeltats3 require the crb2+/rhp9+ gene in fission yeast.

Grenon M et al. Mol Gen Genet 1999 Jan;260(6):522-34

PMID:28441348 - Deoxynucleoside Salvage in Fission Yeast Allows Rescue of Ribonucleotide Reductase Deficiency but Not Spd1-Mediated Inhibition of Replication.

Fleck O et al. Genes (Basel) 2017 Apr 25;8(5)

PMID:25313826 - The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.

Pietrobon V et al. PLoS Biol 2014 Oct;12(10):e1001968

PMID:20452294 - Ubiquitin-PCNA fusion as a mimic for mono-ubiquitinated PCNA in Schizosaccharomyces pombe.

Ramasubramanyan S et al. DNA Repair (Amst) 2010 Jul 01;9(7):777-84

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:12124382 - The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta.

Bermudez VP et al. J Biol Chem 2002 Sep 27;277(39):36853-62

PMID:38261971 - Checkpoint activation by Spd1: a competition-based system relying on tandem disordered PCNA binding motifs.

Olsen JG et al. Nucleic Acids Res 2024 Jan 23;

PMID:10748208 - Fidelity of eucaryotic DNA polymerase delta holoenzyme from Schizosaccharomyces pombe.

Chen X et al. J Biol Chem 2000 Jun 09;275(23):17677-82

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:14752051 - Functional analysis of subcellular localization and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast.

Martin IV et al. Nucleic Acids Res 2004;32(2):632-42

PMID:17304223 - Replication foci dynamics: replication patterns are modulated by S-phase checkpoint kinases in fission yeast.

Meister P et al. EMBO J 2007 Mar 07;26(5):1315-26

PMID:28481910 - PCNA ubiquitylation ensures timely completion of unperturbed DNA replication in fission yeast.

Daigaku Y et al. PLoS Genet 2017 May;13(5):e1006789

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:9794798 - Mutant DNA polymerase delta from thermosensitive Schizosaccharomyces pombe strains display reduced stimulation by proliferating cell nuclear antigen.

Perderiset M et al. Biochem J 1998 Nov 01;335 ( Pt 3)(Pt 3):581-8

PMID:22464192 - Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen.

Salguero I et al. Curr Biol 2012 Apr 24;22(8):720-6

PMID:17515930 - Rad3-dependent phosphorylation of the checkpoint clamp regulates repair-pathway choice.

Kai M et al. Nat Cell Biol 2007 Jun;9(6):691-7

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs

PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.

Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95

PMID:38376141 - Disordered regions and folded modules in CAF-1 promote histone deposition in Schizosaccharomyces pombe .

Ouasti F et al. Elife 2024 Feb 20;12

PMID:21493688 - Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.

Guarino E et al. Nucleic Acids Res 2011 Aug;39(14):5978-90

PMID:22064477 - The RecQ4 orthologue Hrq1 is critical for DNA interstrand cross-link repair and genome stability in fission yeast.

Groocock LM et al. Mol Cell Biol 2012 Jan;32(2):276-87

PMID:19158664 - Smc5/6 maintains stalled replication forks in a recombination-competent conformation.

Irmisch A et al. EMBO J 2009 Jan 21;28(2):144-55

PMID:10449724 - The C-terminal region of Schizosaccaromyces pombe proliferating cell nuclear antigen is essential for DNA polymerase activity.

Kelman Z et al. Proc Natl Acad Sci U S A 1999 Aug 17;96(17):9515-20

PMID:9070271 - PCNA and DNA polymerase delta catalytic subunit from Schizosaccharomyces pombe do not interact directly.

Tratner I et al. Biochem Biophys Res Commun 1997 Feb 13;231(2):321-8

PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.

Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063

PMID:31563844 - The roles of fission yeast exonuclease 5 in nuclear and mitochondrial genome stability.

Sparks JL et al. DNA Repair (Amst) 2019 Nov;83:102720

PMID:7780738 - A small peptide inhibitor of DNA replication defines the site of interaction between the cyclin-dependent kinase inhibitor p21WAF1 and proliferating cell nuclear antigen.

Warbrick E et al. Curr Biol 1995 Mar 01;5(3):275-82

PMID:16407242 - An evolutionarily conserved function of proliferating cell nuclear antigen for Cdt1 degradation by the Cul4-Ddb1 ubiquitin ligase in response to DNA damage.

Hu J et al. J Biol Chem 2006 Feb 17;281(7):3753-6

PMID:22308326 - Tight coevolution of proliferating cell nuclear antigen (PCNA)-partner interaction networks in fungi leads to interspecies network incompatibility.

Zamir L et al. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):E406-14

PMID:32692737 - CRL4Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation.

Zhang JM et al. PLoS Genet 2020 Jul;16(7):e1008933

PMID:10779336 - Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase.

Tanaka K et al. Mol Cell Biol 2000 May;20(10):3459-69

PMID:27151298 - Orderly progression through S-phase requires dynamic ubiquitylation and deubiquitylation of PCNA.

Álvarez V et al. Sci Rep 2016 May 06;6:25513

PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.

Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53

PMID:8663159 - Schizosaccharomyces pombe proliferating cell nuclear antigen mutations affect DNA polymerase delta processivity.

Arroyo MP et al. J Biol Chem 1996 Jul 05;271(27):15971-80

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:16641370 - Postreplication repair and PCNA modification in Schizosaccharomyces pombe.

Frampton J et al. Mol Biol Cell 2006 Jul;17(7):2976-85

PMID:9891039 - Fission yeast cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion.

Tanaka H et al. Mol Cell Biol 1999 Feb;19(2):1038-48

PMID:20453833 - Rad8Rad5/Mms2-Ubc13 ubiquitin ligase complex controls translesion synthesis in fission yeast.

Coulon S et al. EMBO J 2010 Jun 16;29(12):2048-58

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.

McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:12237855 - Genome-wide search of Schizosaccharomyces pombe genes causing overexpression-mediated cell cycle defects.

Tallada VA et al. Yeast 2002 Sep 30;19(13):1139-51

PMID:18761674 - Fission yeast chromatin assembly factor 1 assists in the replication-coupled maintenance of heterochromatin.

Dohke K et al. Genes Cells 2008 Oct;13(10):1027-43

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:12930957 - Nuclear factories for signalling and repairing DNA double strand breaks in living fission yeast.

Meister P et al. Nucleic Acids Res 2003 Sep 01;31(17):5064-73

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:16407840 - A functional analysis of PCNA-binding peptides derived from protein sequence, interaction screening and rational design.

Warbrick E Oncogene 2006 May 11;25(20):2850-9

PMID:34292936 - Fission yeast Rad8/HLTF facilitates Rad52-dependent chromosomal rearrangements through PCNA lysine 107 ubiquitination.

Su J et al. PLoS Genet 2021 Jul;17(7):e1009671

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:8537450 - DNA polymerase alpha, a component of the replication initiation complex, is essential for the checkpoint coupling S phase to mitosis in fission yeast.

D'Urso G et al. J Cell Sci 1995 Sep;108 ( Pt 9):3109-18

PMID:31149897 - The PCNA unloader Elg1 promotes recombination at collapsed replication forks in fission yeast.

Tamang S et al. Elife 2019 May 31;8

PMID:28053344 - Histone chaperone networks shaping chromatin function.

Hammond CM et al. Nat Rev Mol Cell Biol 2017 Mar;18(3):141-158

PMID:1361173 - Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.

Waseem NH et al. EMBO J 1992 Dec;11(13):5111-20

PMID:41330900 - PolySUMOylation of PCNA and Rad52 restricts centromeric recombination in fission yeast.

Markowska K et al. Nat Commun 2025 Dec 02;16(1):10837

PMID:9356477 - Schizosaccharomyces pombe cdc20+ encodes DNA polymerase epsilon and is required for chromosomal replication but not for the S phase checkpoint.

D'Urso G et al. Proc Natl Acad Sci U S A 1997 Nov 11;94(23):12491-6