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protein coding gene - php4 (SPBC16E9.01c) - CCAAT-binding factor complex repressor Php4

Gene summary

Standard name
php4
Systematic ID
SPBC16E9.01c
Product
CCAAT-binding factor complex repressor Php4
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBP16F5.09c
UniProt ID
O14318
ORFeome ID
32/32A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1915375..1916892 reverse strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

PBO:0003817 - promoter contains IDP (GATA)

References:

GO biological process

GO:0006879 - intracellular iron ion homeostasis

References:

GO:0000122 - negative regulation of transcription by RNA polymerase II

References:

GO cellular component

GO:0016602 - CCAAT-binding factor complex

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GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

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GO:0072686 - mitotic spindle

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GO:0005634 - nucleus

References:

GO molecular function

GO:0003714 - transcription corepressor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0002014 - increased RNA level during cellular response to iron ion starvation

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001409 - normal growth on glycerol carbon source

References:

Genotypes:

FYPO:0007121 - normal growth on iron

References:

Genotypes:

FYPO:0000840 - normal RNA level

References:

Genotypes:

FYPO:0002015 - sensitive to iron ion starvation

References:

Genotypes:

Protein sequence feature

SO:0001531 - nuclear_export_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000684 - decreased cell population growth on glycerol carbon source

References:

Genotypes:

FYPO:0000519 - decreased oxidative phosphorylation

References:

Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0006100 - decreased protein localization to cytoplasm, with protein mislocalized to nucleus

References:

Genotypes:

FYPO:0002768 - decreased protein ubiquitination during vegetative growth

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0001117 - decreased RNA level during vegetative growth

References:

Genotypes:

FYPO:0005733 - increased glutamate-ammonia ligase activity during cellular response to iron ion starvation

References:

Genotypes:

FYPO:0001327 - increased protein level during vegetative growth

References:

Genotypes:

FYPO:0003914 - increased protein level in stationary phase

References:

Genotypes:

FYPO:0001890 - increased RNA level

References:

Genotypes:

FYPO:0002014 - increased RNA level during cellular response to iron ion starvation

References:

Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

References:

Genotypes:

FYPO:0001814 - normal cell population growth during iron starvation

References:

Genotypes:

FYPO:0001164 - normal growth on glucose carbon source

References:

Genotypes:

FYPO:0001409 - normal growth on glycerol carbon source

References:

Genotypes:

FYPO:0007121 - normal growth on iron

References:

Genotypes:

FYPO:0006948 - normal protein level during glucose starvation

References:

Genotypes:

FYPO:0007952 - normal protein level during stationary phase

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0005639 - normal RNA level during cellular response to iron ion starvation

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0005825 - sensitive to iron

References:

Genotypes:

FYPO:0002015 - sensitive to iron ion starvation

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10297Hap4_Hap_bindHap4_TF_heteromerisationPfam
CoilCOILS
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMobiDB-Low-complexity

Orthologs

References / Literature

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:23115244 - Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.
Gabrielli N et al. J Biol Chem 2012 Dec 14;287(51):43042-51
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:38360270 - A KDELR-mediated ER-retrieval system modulates mitochondrial functions via the unfolded protein response in fission yeast.
Zhu M et al. J Biol Chem 2024 Feb 13;:105754
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20435771 - Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.
Mercier A et al. Appl Environ Microbiol 2010 Jun;76(12):3806-17
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:42489909 - Functional characterization of Hul6 reveals a role in iron-dependent metabolism in Schizosaccharomyces pombe.
Li R et al. Arch Microbiol 2026 Jul 23;208(10)
PMID:18223116 - Key function for the CCAAT-binding factor Php4 to regulate gene expression in response to iron deficiency in fission yeast.
Mercier A et al. Eukaryot Cell 2008 Mar;7(3):493-508
PMID:24897379 - Fra2 is a co-regulator of Fep1 inhibition in response to iron starvation.
Jacques JF et al. PLoS One 2014;9(6):e98959
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:34086083 - Expression of Mug14 is regulated by the transcription factor Rst2 through the cAMP-dependent protein kinase pathway in Schizosaccharomyces pombe.
Inamura SI et al. Curr Genet 2021 Oct;67(5):807-821
PMID:38499131 - The deletion of ppr2 interferes iron sensing and leads to oxidative stress response in Schizosaccharomyces pombe.
Liu Z et al. Mitochondrion 2024 Mar 16;:101875
PMID:25806539 - A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.
Encinar del Dedo J et al. PLoS Genet 2015 Mar;11(3):e1005106
PMID:36794724 - Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis.
Garg A et al. Nucleic Acids Res 2023 Apr 24;51(7):3094-3115
PMID:19915076 - abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.
Pouliot B et al. Eukaryot Cell 2010 Jan;9(1):59-73
PMID:19502236 - Both Php4 function and subcellular localization are regulated by iron via a multistep mechanism involving the glutaredoxin Grx4 and the exportin Crm1.
Mercier A et al. J Biol Chem 2009 Jul 24;284(30):20249-62
PMID:28725905 - Schizosaccharomyces pombe Grx4 regulates the transcriptional repressor Php4 via [2Fe-2S] cluster binding.
Dlouhy AC et al. Metallomics 2017 Aug 16;9(8):1096-1105
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:36568394 - Schizosaccharomyces pombe Grx4, Fep1, and Php4: In silico analysis and expression response to different iron concentrations.
Ebrahim A et al. Front Genet 2022;13:1069068
PMID:25330182 - Characterization of the nuclear import mechanism of the CCAAT-regulatory subunit Php4.
Khan MG et al. PLoS One 2014;9(10):e110721
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:22523368 - The monothiol glutaredoxin Grx4 exerts an iron-dependent inhibitory effect on Php4 function.
Vachon P et al. Eukaryot Cell 2012 Jun;11(6):806-19
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:38272226 - E3 ubiquitin ligase Hul6 modulates iron-dependent metabolism by regulating Php4 stability.
Yao R et al. J Biol Chem 2024 Jan 23;:105670
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:16963626 - A transcription factor cascade involving Fep1 and the CCAAT-binding factor Php4 regulates gene expression in response to iron deficiency in the fission yeast Schizosaccharomyces pombe.
Mercier A et al. Eukaryot Cell 2006 Nov;5(11):1866-81
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:35657410 - Loss of PPR protein Ppr2 induces ferroptosis-like cell death in Schizosaccharomyces pombe.
Liu Z et al. Arch Microbiol 2022 Jun 03;204(7):360
PMID:41974504 - Nuclear basket subunits Nup211 and Rsm1 influence RNA 3'-processing and transcription termination in fission yeast.
Bednor L et al. RNA 2026 Apr 13;