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protein coding gene - pub3 (SPBC16E9.11c) - HECT-type ubiquitin-protein ligase E3 Pub3

Gene summary

Standard name
pub3
Systematic ID
SPBC16E9.11c
Product
HECT-type ubiquitin-protein ligase E3 Pub3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O14326
ORFeome ID
39/39A04
Characterisation status
biological role inferred
Feature type
mRNA gene
Genomic location
chromosome II: 1935530..1938570 reverse strand

Annotation

Disease association

MONDO:0008323 - Liddle syndrome

References:

MONDO:0014966 - periventricular nodular heterotopia 7

References:

GO biological process

GO:0120113 - cytoplasm to vacuole targeting by the NVT pathway

References:

GO:0006511 - ubiquitin-dependent protein catabolic process

References:

GO cellular component

GO:0005737 - cytoplasm

References:

GO molecular function

GO:0005543 - phospholipid binding

References:

GO:0061630 - ubiquitin protein ligase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:01148 - ubiquitinylated lysine

References:

Protein features

PBO:0111864 - C2 domain

PBO:0111873 - Nedd4/Rsp5 family

References:

PBO:0111749 - ubiquitin-protein ligase E3

PBO:0111769 - WW domain

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0006010 - abolished shmoo contact with partner cell

References:

Genotypes:

FYPO:0000708 - decreased mating efficiency

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0001023 - normal growth on cisplatin

References:

Genotypes:

FYPO:0009031 - resistance to bleomycin

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009083 - resistance to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000830 - resistance to vanadate

References:

Genotypes:

FYPO:0001501 - sensitive to brefeldin A

References:

Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0000797 - sensitive to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0000115 - sensitive to valproic acid

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF00397WWWW_domPFAM
PF00168C2C2_domPFAM
PF00632HECTHECT_domPFAM
cd00201WWWW_domCDD
cd00078HECTcHECT_domCDD
cd08382C2_Smurf-likeCDD
PS01159WW_DOMAIN_1WW_domPROSITE_PATTERNS
PS50020WW_DOMAIN_2WW_domPROSITE_PROFILES
PS50237HECTHECT_domPROSITE_PROFILES
PS50004C2C2_domPROSITE_PROFILES
SM00239C2_3cC2_domSMART
SM00119hect_3HECT_domSMART
SM00456ww_5WW_domSMART
G3DSA:3.30.2160.10:FF:000001FUNFAM
G3DSA:2.20.70.10:FF:000017FUNFAM
G3DSA:3.90.1750.10:FF:000005FUNFAM
G3DSA:3.30.2410.10:FF:000001FUNFAM
SSF49562C2 domain (Calcium/lipid-binding domain, CaLB)C2_domain_sfSUPERFAMILY
SSF51045WW domainWW_dom_sfSUPERFAMILY
SSF56204Hect, E3 ligase catalytic domainHect_E3_ubiquitin_ligaseSUPERFAMILY
G3DSA:3.30.2160.10Hect, E3 ligase catalytic domainGENE3D
G3DSA:2.20.70.10GENE3D
G3DSA:3.30.2410.10Hect, E3 ligase catalytic domainGENE3D
G3DSA:3.90.1750.10Hect, E3 ligase catalytic domainsGENE3D
G3DSA:2.60.40.150C2 domainC2_domain_sfGENE3D
PTHR11254HECT DOMAIN UBIQUITIN-PROTEIN LIGASEE3_ubiq-protein_ligasePANTHER
PIRSF001569E3_ub_ligase_SMURF1E3_ub_ligase_SMURF1PIRSF
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Roguev A et al. Science 2008 Oct 17;322(5900):405-10
PMID:21247416 - Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast.
Gatti L et al. BMC Genomics 2011 Jan 19;12:44
PMID:22194353 - Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p.
Nakase M et al. Microbiology (Reading) 2012 Mar;158(Pt 3):659-673
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
GO_REF:0000050 - Manual transfer of GO annotation data to genes by curator judgment of sequence model
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:24454826 - E3 ubiquitin ligase Pub1 is implicated in endocytosis of a GPI-anchored protein Ecm33 in fission yeast.
Fang Y et al. PLoS One 2014;9(1):e85238
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PB_REF:0000003 - Disease Association Curation
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:11956316 - The novel HECT-type ubiquitin-protein ligase Pub2p shares partially overlapping function with Pub1p in Schizosaccharomyces pombe.
Tamai KK et al. J Cell Sci 2002 May 01;115(Pt 9):1847-57
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;