protein coding gene - rpn12 (SPBC16G5.01) - 19S proteasome regulatory subunit Rpn12

Gene summary

Standard name

rpn12

Systematic ID

SPBC16G5.01

Product

19S proteasome regulatory subunit Rpn12

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

mts3, SPBC342.07

UniProt ID

P50524

ORFeome ID

32/32A04

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 4214977..4216117 forward strand

Annotation

Protein features

Orthologs

• PSMD8 (HGNC:9566) Homo sapiens
• rpn12 (SJAG_01203) Schizosaccharomyces japonicus
• RPN12 (YFR052W) Saccharomyces cerevisiae

References / Literature

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:18023413 - Dss1 associating with the proteasome functions in selective nuclear mRNA export in yeast.

Mannen T et al. Biochem Biophys Res Commun 2008 Jan 25;365(4):664-71

PMID:21098295 - Structure of the 26S proteasome from Schizosaccharomyces pombe at subnanometer resolution.

Bohn S et al. Proc Natl Acad Sci U S A 2010 Dec 07;107(49):20992-7

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:10082519 - The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function.

Berry LD et al. Mol Cell Biol 1999 Apr;19(4):2535-46

PMID:12553909 - Schizosaccharomyces pombe Int6 and Ras homologs regulate cell division and mitotic fidelity via the proteasome.

Yen HC et al. Cell 2003 Jan 24;112(2):207-17

PMID:22906049 - Structural and functional characterization of Rpn12 identifies residues required for Rpn10 proteasome incorporation.

Boehringer J et al. Biochem J 2012 Nov 15;448(1):55-65

PMID:26221037 - Pli1(PIAS1) SUMO ligase protected by the nuclear pore-associated SUMO protease Ulp1SENP1/2.

Nie M et al. J Biol Chem 2015 Sep 11;290(37):22678-85

PMID:38285941 - Specialized replication of heterochromatin domains ensures self-templated chromatin assembly and epigenetic inheritance.

Nathanailidou P et al. Proc Natl Acad Sci U S A 2024 Feb 06;121(6):e2315596121

PMID:8621436 - A conditional lethal mutant in the fission yeast 26 S protease subunit mts3+ is defective in metaphase to anaphase transition.

Gordon C et al. J Biol Chem 1996 Mar 08;271(10):5704-11

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:15933715 - Etd1p is a novel protein that links the SIN cascade with cytokinesis.

Daga RR et al. EMBO J 2005 Jul 06;24(13):2436-46

PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.

May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228

PMID:20739285 - Structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12.

Riedinger C et al. J Biol Chem 2010 Oct 29;285(44):33992-4003

PMID:25487150 - A PP1-PP2A phosphatase relay controls mitotic progression.

Grallert A et al. Nature 2015 Jan 01;517(7532):94-98

PMID:11084332 - Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2.

Tatebe H et al. Curr Biol 2000 Nov 02;10(21):1329-38

PMID:32915139 - Stress-activated MAPK signaling controls fission yeast actomyosin ring integrity by modulating formin For3 levels.

Gómez-Gil E et al. Elife 2020 Sep 11;9

PMID:9430640 - Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor.

Benito J et al. EMBO J 1998 Jan 15;17(2):482-97

PMID:28784663 - The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast.

Seo HD et al. J Biol Chem 2017 Oct 13;292(41):17144-17155

PMID:41844616 - Phosphoregulation of the novel hemi-arrestin MAPK scaffold Sms1 prevents untimely mating.

Sieber B et al. Nat Commun 2026 Mar 17;

PMID:21091378 - Txl1 and Txc1 are co-factors of the 26S proteasome in fission yeast.

Andersen KM et al. Antioxid Redox Signal 2011 May 01;14(9):1601-8

PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.

Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870

PMID:27655872 - A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP.

Hwang J et al. EMBO J 2016 Nov 02;35(21):2332-2349

PMID:22144913 - Widespread cotranslational formation of protein complexes.

Duncan CD et al. PLoS Genet 2011 Dec;7(12):e1002398

PMID:29975113 - Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization.

Jones CM et al. Mol Biol Cell 2018 Sep 01;29(18):2176-2189

PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.

Le TK et al. J Cell Sci 2023 Oct 01;136(19)

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:24938783 - Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.

Leitner A et al. Proc Natl Acad Sci U S A 2014 Jul 01;111(26):9455-60

PMID:9653157 - sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast.

Jallepalli PV et al. Proc Natl Acad Sci U S A 1998 Jul 07;95(14):8159-64

PMID:23209828 - Fission yeast 26S proteasome mutants are multi-drug resistant due to stabilization of the Pap1 transcription factor.

Penney M et al. PLoS One 2012;7(11):e50796

PMID:17761670 - Isolation of the Schizosaccharomyces pombe proteasome subunit Rpn7 and a structure-function study of the proteasome-COP9-initiation factor domain.

Sha Z et al. J Biol Chem 2007 Nov 02;282(44):32414-23

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:20133687 - Synergistic roles of the proteasome and autophagy for mitochondrial maintenance and chronological lifespan in fission yeast.

Takeda K et al. Proc Natl Acad Sci U S A 2010 Feb 23;107(8):3540-5

PMID:22451489 - Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast.

Szilagyi Z et al. Mol Cell Biol 2012 Jun;32(11):2099-109

PMID:18062930 - Functional characterisation of the Schizosaccharomyces pombe homologue of the leukaemia-associated translocation breakpoint binding protein translin and its binding partner, TRAX.

Jaendling A et al. Biochim Biophys Acta 2008 Feb;1783(2):203-13

PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.

Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9

PMID:24710126 - The 19S proteasome subunit Rpt3 regulates distribution of CENP-A by associating with centromeric chromatin.

Kitagawa T et al. Nat Commun 2014 Apr 07;5:3597

PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.

Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71

PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.

Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323

PMID:20838651 - A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity.

Kouranti I et al. PLoS Biol 2010 Sep 07;8(9)

PMID:15533439 - Uch2/Uch37 is the major deubiquitinating enzyme associated with the 26S proteasome in fission yeast.

Stone M et al. J Mol Biol 2004 Nov 26;344(3):697-706

PMID:16096059 - Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8.

Takeda K et al. Cell 2005 Aug 12;122(3):393-405

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:10809753 - Analysis of a gene encoding Rpn10 of the fission yeast proteasome reveals that the polyubiquitin-binding site of this subunit is essential when Rpn12/Mts3 activity is compromised.

Wilkinson CR et al. J Biol Chem 2000 May 19;275(20):15182-92

PMID:16904908 - Cdc48 is required for the stability of Cut1/separase in mitotic anaphase.

Ikai N et al. J Struct Biol 2006 Oct;156(1):50-61

PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.

Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71

PMID:9727008 - The Pad1+ gene encodes a subunit of the 26 S proteasome in fission yeast.

Penney M et al. J Biol Chem 1998 Sep 11;273(37):23938-45

PMID:38051102 - Fission Yeast TORC1 Promotes Cell Proliferation through Sfp1, a Transcription Factor Involved in Ribosome Biogenesis.

Tai YT et al. Mol Cell Biol 2023 Dec 05;:1-18

PMID:8918598 - Characteristics of 26 S proteases from fission yeast mutants, which arrest in mitosis.

Seeger M et al. J Mol Biol 1996 Nov 01;263(3):423-31

PMID:12511578 - Identification of Uhp1, a ubiquitinated histone-like protein, as a target/mediator of Rhp6 in mating-type silencing in fission yeast.

Naresh A et al. J Biol Chem 2003 Mar 14;278(11):9185-94

PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.

Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213

PMID:10582241 - Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization.

Yanagida M et al. Philos Trans R Soc Lond B Biol Sci 1999 Sep 29;354(1389):1559-69; discussion 1569-70

PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.

Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99

PMID:16149916 - Fission yeast Dss1 associates with the proteasome and is required for efficient ubiquitin-dependent proteolysis.

Jossé L et al. Biochem J 2006 Jan 01;393(Pt 1):303-9

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:24006256 - Fission yeast nucleolar protein Dnt1 regulates G2/M transition and cytokinesis by downregulating Wee1 kinase.

Yu ZY et al. J Cell Sci 2013 Nov 01;126(Pt 21):4995-5004

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:9203581 - Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18.

Kominami K et al. Genes Dev 1997 Jun 15;11(12):1548-60

PMID:15660136 - SCF(Pof1)-ubiquitin and its target Zip1 transcription factor mediate cadmium response in fission yeast.

Harrison C et al. EMBO J 2005 Feb 09;24(3):599-610

PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.

Lee J et al. Nat Commun 2017 Feb 20;8:14527

PMID:16252005 - Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.

Liu C et al. EMBO J 2005 Nov 16;24(22):3940-51

PMID:12668659 - An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation.

Tasto JJ et al. J Cell Biol 2003 Mar 31;160(7):1093-103

PMID:12783882 - Rpn5 is a conserved proteasome subunit and required for proper proteasome localization and assembly.

Yen HC et al. J Biol Chem 2003 Aug 15;278(33):30669-76