PomBase home

protein coding gene - top3 (SPBC16G5.12c) - DNA topoisomerase III

Gene summary

Standard name
top3
Systematic ID
SPBC16G5.12c
Product
DNA topoisomerase III
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O60126
ORFeome ID
40/40H04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4233868..4236269 reverse strand

Annotation

PBO:0004270 - 5.99.1.2

Disease association

MONDO:0020628 - microcephaly, growth restriction, and increased sister chromatid exchange 2

References:

MONDO:0020845 - progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 5

References:

GO biological process

GO:0006301 - DNA damage tolerance

References:

GO:0006281 - DNA repair

References:

GO:0006265 - DNA topological change

References:

GO:0035825 - homologous recombination

References:

GO:0033260 - nuclear DNA replication

References:

GO:0045458 - recombination within rDNA repeats

References:

GO cellular component

GO:0005634 - nucleus

References:

GO:0031422 - RecQ family helicase-topoisomerase III complex

References:

GO:0035861 - site of double-strand break

References:

GO molecular function

GO:0003677 - DNA binding

References:

GO:0003917 - DNA topoisomerase type I (single strand cut, ATP-independent) activity

References:

Multi-locus phenotype

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001054 - cut, elongated cell

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0001494 - inviable elongated multiseptate vegetative cell

References:

Genotypes:

FYPO:0002150 - inviable spore population

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0005704 - loss of viability following cellular response to hydroxyurea

References:

Genotypes:

FYPO:0005705 - loss of viability following cellular response to UV

References:

Genotypes:

FYPO:0000405 - normal mitotic G2/M phase transition

References:

Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0003503 - normal vegetative cell length

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0000267 - sensitive to ionizing radiation during vegetative growth

References:

Genotypes:

FYPO:0003612 - viable spore population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000029 - abnormal chromosome segregation

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000006 - abnormal mitotic DNA damage checkpoint

References:

Genotypes:

FYPO:0000361 - abnormal nucleolar morphology

References:

Genotypes:

FYPO:0000446 - cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0003306 - decreased mitotic index

References:

Genotypes:

FYPO:0006663 - elongated nucleus

References:

Genotypes:

FYPO:0001122 - elongated vegetative cell

References:

Genotypes:

FYPO:0006661 - fragmented nucleus

References:

Genotypes:

FYPO:0003970 - incomplete mitotic sister chromatid segregation, with chromatin bridge

References:

Genotypes:

FYPO:0008162 - increased activation of S-phase DNA damage checkpoint

References:

Genotypes:

FYPO:0004355 - increased protein phosphorylation during mitosis

References:

Genotypes:

FYPO:0004627 - inviable after spore germination, multiple cell divisions, chromosome fragmentation upon segregation

References:

Genotypes:

FYPO:0002262 - inviable after spore germination, multiple cell divisions, elongated cell

References:

Genotypes:

FYPO:0003763 - inviable aseptate mononucleate vegetative cell

References:

Genotypes:

FYPO:0004922 - inviable elongated mononucleate aseptate cell with cell cycle arrest at mitotic G2/M phase transition

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002000 - inviable septated mononucleate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0006715 - large and small daughter nuclei

References:

Genotypes:

FYPO:0003438 - mitotic G1/S phase transition delay following nitrogen starvation-induced G1 phase arrest

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0001761 - normal mitotic G1/S phase transition

References:

Genotypes:

FYPO:0001513 - normal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001581 - vacuolated

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF01131Topoisom_bacTopo_IA_cenPFAM
PF01751ToprimTOPRIM_domPFAM
cd00186TOP1AcTopo_IA_cenCDD
cd03362TOPRIM_TopoIA_TopoIIITOPRIM_TopoIIICDD
PS00396TOPO_IA_1Topo_IA_ASPROSITE_PATTERNS
PS50880TOPRIMTOPRIM_domPROSITE_PROFILES
PS52039TOPO_IA_2Topo_IA_cenPROSITE_PROFILES
SM00437topIaneu2Topo_IA_DNA-bd_domSMART
SM00436topIban2Topo_IA_2SMART
SM00493toprim5TOPRIM_domSMART
PR00417PRTPISMRASEITopo_IA_cenPRINTS
G3DSA:1.10.290.10:FF:000001FUNFAM
G3DSA:3.40.50.140:FF:000003FUNFAM
SSF56712Prokaryotic type I DNA topoisomeraseTopo_IA_core_domainSUPERFAMILY
G3DSA:1.10.460.10Topoisomerase I, domain 2Topo_IA_cen_sub1GENE3D
G3DSA:2.70.20.10Topoisomerase I, domain 3Topo_IA_cen_sub2GENE3D
G3DSA:3.40.50.140GENE3D
G3DSA:1.10.290.10Topoisomerase I, domain 4Topo_IA_cen_sub3GENE3D
PTHR11390PROKARYOTIC DNA TOPOISOMERASETopo_IAPANTHER

Orthologs

References / Literature

PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:12724426 - Role for the fission yeast RecQ helicase in DNA repair in G2.
Laursen LV et al. Mol Cell Biol 2003 May;23(10):3692-705
PMID:12023299 - Cdc2-cyclin B kinase activity links Crb2 and Rqh1-topoisomerase III.
Caspari T et al. Genes Dev 2002 May 15;16(10):1195-208
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:12409469 - The severe slow growth of Deltasrs2 Deltarqh1 in Schizosaccharomyces pombe is suppressed by loss of recombination and checkpoint genes.
Maftahi M et al. Nucleic Acids Res 2002 Nov 01;30(21):4781-92
PMID:10572171 - The top3(+) gene is essential in Schizosaccharomyces pombe and the lethality associated with its loss is caused by Rad12 helicase activity.
Maftahi M et al. Nucleic Acids Res 1999 Dec 15;27(24):4715-24
GO_REF:0000002 - Comments
PMID:12509234 - Inactivation of homologous recombination suppresses defects in topoisomerase III-deficient mutants.
Oakley TJ et al. DNA Repair (Amst) 2002 Jun 21;1(6):463-82
PMID:15702347 - The N-terminal region of the Schizosaccharomyces pombe RecQ helicase, Rqh1p, physically interacts with Topoisomerase III and is required for Rqh1p function.
Ahmad F et al. Mol Genet Genomics 2005 Mar;273(1):102-14
PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:18031226 - The role of Schizosaccharomyces pombe SUMO ligases in genome stability.
Watts FZ et al. Biochem Soc Trans 2007 Dec;35(Pt 6):1379-84
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:17178839 - Fission yeast Cut8 is required for the repair of DNA double-strand breaks, ribosomal DNA maintenance, and cell survival in the absence of Rqh1 helicase.
Kearsey SE et al. Mol Cell Biol 2007 Mar;27(5):1558-67
PMID:12235386 - Topoisomerase III is required for accurate DNA replication and chromosome segregation in Schizosaccharomyces pombe.
Oh M et al. Nucleic Acids Res 2002 Sep 15;30(18):4022-31
PMID:23628481 - A proteome-wide visual screen identifies fission yeast proteins localizing to DNA double-strand breaks.
Yu Y et al. DNA Repair (Amst) 2013 Jun 01;12(6):433-43
PMID:15340008 - Requirement for Schizosaccharomyces pombe Top3 in the maintenance of chromosome integrity.
Win TZ et al. J Cell Sci 2004 Sep 15;117(Pt 20):4769-78
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
PMID:10497270 - Topoisomerase III is essential for accurate nuclear division in Schizosaccharomyces pombe.
Goodwin A et al. Nucleic Acids Res 1999 Oct 15;27(20):4050-8
PMID:23516381 - DNA topoisomerase III localizes to centromeres and affects centromeric CENP-A levels in fission yeast.
Norman-Axelsson U et al. PLoS Genet 2013;9(3):e1003371
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83