protein coding gene - top1 (SPBC1703.14c) - DNA topoisomerase I

Gene summary

Standard name

top1

Systematic ID

SPBC1703.14c

Product

DNA topoisomerase I

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

P07799

ORFeome ID

33/33H03

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 2941734..2944596 reverse strand

Annotation

Protein features

Orthologs

• TOP1 (HGNC:11986) Homo sapiens
• TOP1MT (HGNC:29787) Homo sapiens
• top1 (SJAG_05193) Schizosaccharomyces japonicus
• TOP1 (YOL006C) Saccharomyces cerevisiae

References / Literature

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:18684775 - A genome-wide screen of genes involved in cadmium tolerance in Schizosaccharomyces pombe.

Kennedy PJ et al. Toxicol Sci 2008 Nov;106(1):124-39

PMID:23703609 - TORC2 is required to maintain genome stability during S phase in fission yeast.

Schonbrun M et al. J Biol Chem 2013 Jul 05;288(27):19649-60

PMID:10712506 - Mechanisms of sod2 gene amplification in Schizosaccharomyces pombe.

Albrecht EB et al. Mol Biol Cell 2000 Mar;11(3):873-86

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:22730331 - Dual recruitment of Cdc48 (p97)-Ufd1-Npl4 ubiquitin-selective segregase by small ubiquitin-like modifier protein (SUMO) and ubiquitin in SUMO-targeted ubiquitin ligase-mediated genome stability functions.

Nie M et al. J Biol Chem 2012 Aug 24;287(35):29610-9

PMID:2436053 - Need for DNA topoisomerase activity as a swivel for DNA replication for transcription of ribosomal RNA.

Brill SJ et al. Nature 1987 Mar 26;326(6111):414-6

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.

Liu NN et al. Cell Res 2010 Aug;20(8):963-5

PMID:6090122 - Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

Uemura T et al. EMBO J 1984 Aug;3(8):1737-44

PMID:19150433 - Distinct requirements for the Rad32(Mre11) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA.

Hartsuiker E et al. Mol Cell 2009 Jan 16;33(1):117-23

PMID:2827111 - Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption.

Uemura T et al. Nucleic Acids Res 1987 Dec 10;15(23):9727-39

PMID:15957215 - Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis.

Uemura T et al. EMBO J 1986 May;5(5):1003-10

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.

Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7

PMID:19197239 - Tdp1 protects against oxidative DNA damage in non-dividing fission yeast.

Ben Hassine S et al. EMBO J 2009 Mar 18;28(6):632-40

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:10407262 - The topoisomerase I poison camptothecin generates a Chk1-dependent DNA damage checkpoint signal in fission yeast.

Wan S et al. Yeast 1999 Jul;15(10A):821-8

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.

Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:20537132 - Global fitness profiling of fission yeast deletion strains by barcode sequencing.

Han TX et al. Genome Biol 2010;11(6):R60

PMID:22907753 - Posttranscriptional regulation of cell-cell interaction protein-encoding transcripts by Zfs1p in Schizosaccharomyces pombe.

Wells ML et al. Mol Cell Biol 2012 Oct;32(20):4206-14

PMID:12084712 - Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks.

Doe CL et al. J Biol Chem 2002 Sep 06;277(36):32753-9

PMID:20970342 - Virtual breakdown of the nuclear envelope in fission yeast meiosis.

Asakawa H et al. Curr Biol 2010 Nov 09;20(21):1919-25

PMID:22540037 - Predicting the fission yeast protein interaction network.

Pancaldi V et al. G3 (Bethesda) 2012 Apr;2(4):453-67

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:25452419 - Parallel profiling of fission yeast deletion mutants for proliferation and for lifespan during long-term quiescence.

Sideri T et al. G3 (Bethesda) 2014 Dec 01;5(1):145-55

PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.

Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:7957061 - Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis.

Saka Y et al. EMBO J 1994 Oct 17;13(20):4938-52

PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101

PMID:18031226 - The role of Schizosaccharomyces pombe SUMO ligases in genome stability.

Watts FZ et al. Biochem Soc Trans 2007 Dec;35(Pt 6):1379-84

PMID:17307401 - The novel gene mus7(+) is involved in the repair of replication-associated DNA damage in fission yeast.

Yokoyama M et al. DNA Repair (Amst) 2007 Jun 01;6(6):770-80

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.

Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902

PMID:2839305 - Deficiency in both type I and type II DNA topoisomerase activities differentially affect rRNA and ribosomal protein synthesis in Schizosaccharomyces pombe.

Yamagishi M et al. Curr Genet 1988 Apr;13(4):305-14

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:30992049 - The NuA4 acetyltransferase and histone H4 acetylation promote replication recovery after topoisomerase I-poisoning.

Noguchi C et al. Epigenetics Chromatin 2019 Apr 16;12(1):24

PMID:15702347 - The N-terminal region of the Schizosaccharomyces pombe RecQ helicase, Rqh1p, physically interacts with Topoisomerase III and is required for Rqh1p function.

Ahmad F et al. Mol Genet Genomics 2005 Mar;273(1):102-14

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:15548596 - Analysis of mutant phenotypes and splicing defects demonstrates functional collaboration between the large and small subunits of the essential splicing factor U2AF in vivo.

Webb CJ et al. Mol Biol Cell 2005 Feb;16(2):584-96

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:16478992 - Requirement of fission yeast Cid14 in polyadenylation of rRNAs.

Win TZ et al. Mol Cell Biol 2006 Mar;26(5):1710-21

PMID:21408210 - SUMO-targeted ubiquitin ligase, Rad60, and Nse2 SUMO ligase suppress spontaneous Top1-mediated DNA damage and genome instability.

Heideker J et al. PLoS Genet 2011 Mar;7(3):e1001320

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:30824696 - Systematic analysis reveals the prevalence and principles of bypassable gene essentiality.

Li J et al. Nat Commun 2019 Mar 01;10(1):1002

PMID:16453724 - Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis.

Hirano T et al. EMBO J 1986 Nov;5(11):2973-9

PMID:21444718 - DNA repair and global sumoylation are regulated by distinct Ubc9 noncovalent complexes.

Prudden J et al. Mol Cell Biol 2011 Jun;31(11):2299-310

PMID:37956308 - Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery.

Vega M et al. Nucleic Acids Res 2023 Dec 11;51(22):12161-12173

PMID:25203555 - Fission yeast Pxd1 promotes proper DNA repair by activating Rad16XPF and inhibiting Dna2.

Zhang JM et al. PLoS Biol 2014 Sep;12(9):e1001946

PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.

Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595

GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

PMID:2537310 - Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus.

Hirano T et al. J Cell Biol 1989 Feb;108(2):243-53

PMID:2547758 - Mapping of the active site tyrosine of eukaryotic DNA topoisomerase I.

Eng WK et al. J Biol Chem 1989 Aug 15;264(23):13373-6

PMID:11030618 - swi1 and swi3 perform imprinting, pausing, and termination of DNA replication in S. pombe.

Dalgaard JZ et al. Cell 2000 Sep 15;102(6):745-51

PMID:23936535 - Slx8 removes Pli1-dependent protein-SUMO conjugates including SUMOylated topoisomerase I to promote genome stability.

Steinacher R et al. PLoS One 2013;8(8):e71960

PMID:24945319 - CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.

Vanoosthuyse V et al. PLoS Genet 2014 Jun;10(6):e1004415

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:25392932 - RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes.

Legros P et al. PLoS Genet 2014 Nov;10(11):e1004794

PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.

Nie M et al. Sci Rep 2015 Sep 25;5:14389

PMID:18257517 - Phosphoproteome analysis of fission yeast.

Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97

PMID:2849043 - Evidence that DNA topoisomerase I is necessary for the cytotoxic effects of camptothecin.

Eng WK et al. Mol Pharmacol 1988 Dec;34(6):755-60