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protein coding gene - sks2 (SPBC1709.05) - heat shock protein, Hsp70 family, ribosome associated Sks2

Gene summary

Standard name
sks2
Systematic ID
SPBC1709.05
Product
heat shock protein, Hsp70 family, ribosome associated Sks2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
hsc1
UniProt ID
Q10284
ORFeome ID
33/33E06
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 1106396..1108511 forward strand

Annotation

GO biological process

GO:0051083 - 'de novo' cotranslational protein folding

References:

GO:0042026 - protein refolding

References:

GO:0034976 - response to endoplasmic reticulum stress

References:

GO:0042254 - ribosome biogenesis

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0005886 - plasma membrane

References:

GO:0140453 - protein aggregate center

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GO molecular function

GO:0005524 - ATP binding

References:

GO:0016887 - ATP hydrolysis activity

References:

GO:0031072 - heat shock protein binding

References:

GO:0044183 - protein folding chaperone

References:

GO:0051082 - unfolded protein binding

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Miscellaneous functional group

PBO:0000255 - chaperone

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000020 - protein level unchanged

References:

PomGeneEx:0000021 - protein present

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PomGeneEx:0000027 - ribosomal density decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PomGeneEx:0000014 - RNA present

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

References:

Genotypes:

FYPO:0007299 - decreased protein aggregate center formation

References:

Genotypes:

FYPO:0004806 - incomplete cell wall disassembly at cell fusion site

References:

Genotypes:

FYPO:0000245 - loss of viability in stationary phase

References:

Genotypes:

FYPO:0003706 - normal growth on K-252a

References:

Genotypes:

FYPO:0000067 - resistance to brefeldin A

References:

Genotypes:

FYPO:0000750 - sensitive to silver ions

References:

Genotypes:

FYPO:0006929 - sensitive to silver nanoparticles

References:

Genotypes:

FYPO:0005252 - sensitive to tamoxifen

References:

Genotypes:

FYPO:0007938 - sensitive to tea tree oil

References:

Genotypes:

FYPO:0001234 - slow vegetative cell population growth

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Warnings

PBO:0091709 - ask2 is the name which was given to the spurious ORF on the opposite strand

Protein features

IDNameInterPro nameDB name
PF00012HSP70Hsp_70_famPFAM
PS00329HSP70_2Heat_shock_70_CSPROSITE_PATTERNS
PS01036HSP70_3Heat_shock_70_CSPROSITE_PATTERNS
PS00297HSP70_1Heat_shock_70_CSPROSITE_PATTERNS
PR00301HEATSHOCK70PRINTS
G3DSA:3.30.30.30:FF:000005FUNFAM
G3DSA:3.30.420.40:FF:000172FUNFAM
G3DSA:3.30.420.40:FF:000026FUNFAM
G3DSA:1.20.1270.10:FF:000014FUNFAM
G3DSA:3.90.640.10:FF:000002FUNFAM
G3DSA:2.60.34.10:FF:000004FUNFAM
SSF53067Actin-like ATPase domainATPase_NBDSUPERFAMILY
SSF100934Heat shock protein 70kD (HSP70), C-terminal subdomainHSP70_C_sfSUPERFAMILY
SSF100920Heat shock protein 70kD (HSP70), peptide-binding domainHSP70_peptide-bd_sfSUPERFAMILY
G3DSA:3.90.640.10Actin; Chain A, domain 4GENE3D
G3DSA:1.20.1270.10HSP70_C_sfGENE3D
G3DSA:2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1HSP70_peptide-bd_sfGENE3D
G3DSA:3.30.30.30GENE3D
G3DSA:3.30.420.40GENE3D
PTHR19375HEAT SHOCK PROTEIN 70KDAHsp_70_famPANTHER
CoilCoilCOILS
NF001413PRK00290.1NCBIFAM

Orthologs

References / Literature

PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:23050226 - A genetic screen to discover pathways affecting cohesin function in Schizosaccharomyces pombe identifies chromatin effectors.
Chen Z et al. G3 (Bethesda) 2012 Oct;2(10):1161-8
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:29294138 - Editor's Highlight: A Genome-wide Screening of Target Genes Against Silver Nanoparticles in Fission Yeast.
Lee AR et al. Toxicol Sci 2018 Jan 01;161(1):171-185
PMID:9161410 - A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance.
Usui T et al. Gene 1997 Apr 11;189(1):43-7
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.
Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
GO_REF:0000002 - Comments
PMID:28765280 - The exocyst subunit Sec3 is regulated by a protein quality control pathway.
Kampmeyer C et al. J Biol Chem 2017 Sep 15;292(37):15240-15253
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:15507118 - An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation.
Yuasa T et al. Genes Cells 2004 Nov;9(11):1069-82
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24818994 - The S. pombe translation initiation factor eIF4G is Sumoylated and associates with the SUMO protease Ulp2.
Jongjitwimol J et al. PLoS One 2014;9(5):e94182
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:32075773 - Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress.
Cabrera M et al. Cell Rep 2020 Feb 18;30(7):2430-2443.e4
PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.
Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:37553386 - A conserved membrane curvature-generating protein is crucial for autophagosome formation in fission yeast.
Wang N et al. Nat Commun 2023 Aug 08;14(1):4765
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:24634168 - Proteome-wide search for PP2A substrates in fission yeast.
Bernal M et al. Proteomics 2014 Jun;14(11):1367-80
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:8973306 - Cloning and characterization of hsc1+, a heat shock cognate gene of the fission yeast Schizosaccharomyces pombe.
Oishi K et al. Gene 1996 Nov 28;181(1-2):45-9
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:20085751 - Monothiol glutaredoxin Grx5 interacts with Fe-S scaffold proteins Isa1 and Isa2 and supports Fe-S assembly and DNA integrity in mitochondria of fission yeast.
Kim KD et al. Biochem Biophys Res Commun 2010 Feb 12;392(3):467-72
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:35172472 - Knockdown of vps54 aggravates tamoxifen-induced cytotoxicity in fission yeast.
Lee S et al. Genomics Inform 2021 Dec;19(4):e39
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:27966061 - UBL/BAG-domain co-chaperones cause cellular stress upon overexpression through constitutive activation of Hsf1.
Poulsen EG et al. Cell Stress Chaperones 2017 Jan;22(1):143-154
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399