protein coding gene - rap1 (SPBC1778.02) - shelterin complex scaffold subunit Rap1

Gene summary

Standard name

rap1

Systematic ID

SPBC1778.02

Product

shelterin complex scaffold subunit Rap1

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

lot2

UniProt ID

Q96TL7

ORFeome ID

26/26G09

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3098271..3100698 forward strand

Annotation

Protein features

Orthologs

• TERF2IP (HGNC:19246) Homo sapiens
• rap1 (SJAG_03092) Schizosaccharomyces japonicus
• RAP1 (YNL216W) Saccharomyces cerevisiae

References / Literature

PMID:18160711 - Recombination-based telomere maintenance is dependent on Tel1-MRN and Rap1 and inhibited by telomerase, Taz1, and Ku in fission yeast.

Subramanian L et al. Mol Cell Biol 2008 Mar;28(5):1443-55

PMID:11226171 - Novel functional requirements for non-homologous DNA end joining in Schizosaccharomyces pombe.

Manolis KG et al. EMBO J 2001 Jan 15;20(1-2):210-21

PMID:31131414 - Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.

Inoue H et al. Nucleic Acids Res 2019 Jul 26;47(13):6871-6884

PMID:19214192 - Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres.

Moser BA et al. EMBO J 2009 Apr 08;28(7):810-20

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:26988418 - The proper connection between shelterin components is required for telomeric heterochromatin assembly.

Wang J et al. Genes Dev 2016 Apr 01;30(7):827-39

PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.

Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:30462301 - Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.

Hu C et al. Nucleic Acids Res 2019 Feb 20;47(3):1573-1584

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:16096639 - Taz1, Rap1 and Rif1 act both interdependently and independently to maintain telomeres.

Miller KM et al. EMBO J 2005 Sep 07;24(17):3128-35

PMID:34255844 - The cooperative assembly of shelterin bridge provides a kinetic gateway that controls telomere length homeostasis.

Liu J et al. Nucleic Acids Res 2021 Aug 20;49(14):8110-8119

PMID:22139915 - The telomeric transcriptome of Schizosaccharomyces pombe.

Bah A et al. Nucleic Acids Res 2012 Apr;40(7):2995-3005

PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.

Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101

PMID:27611693 - A Taz1- and Microtubule-Dependent Regulatory Relationship between Telomere and Centromere Positions in Bouquet Formation Secures Proper Meiotic Divisions.

Katsumata K et al. PLoS Genet 2016 Sep;12(9):e1006304

PMID:25398909 - Fission yeast Cactin restricts telomere transcription and elongation by controlling Rap1 levels.

Lorenzi LE et al. EMBO J 2015 Jan 02;34(1):115-29

PMID:24013504 - Tpz1 controls a telomerase-nonextendible telomeric state and coordinates switching to an extendible state via Ccq1.

Jun HI et al. Genes Dev 2013 Sep 01;27(17):1917-31

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:27298342 - Identification of S-phase DNA damage-response targets in fission yeast reveals conservation of damage-response networks.

Willis NA et al. Proc Natl Acad Sci U S A 2016 Jun 28;113(26):E3676-85

PMID:24244195 - Fission yeast shelterin regulates DNA polymerases and Rad3(ATR) kinase to limit telomere extension.

Chang YT et al. PLoS Genet 2013 Nov;9(11):e1003936

PMID:41201383 - Combinatorial Analyses of Pre-mRNA Splicing-Defective Mutants Reveal Differential Quantitative Requirements for Shelterin in Distinct Telomere Functions.

Takeuchi M et al. Genes Cells 2025 Nov;30(6):e70064

PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.

Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146

PMID:29149597 - Structural Basis for Shelterin Bridge Assembly.

Kim JK et al. Mol Cell 2017 Nov 16;68(4):698-714.e5

PMID:22959349 - Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation.

Fujita I et al. Curr Biol 2012 Oct 23;22(20):1932-7

PMID:22354040 - RPA facilitates telomerase activity at chromosome ends in budding and fission yeasts.

Luciano P et al. EMBO J 2012 Apr 18;31(8):2034-46

PMID:21217703 - A conserved motif within RAP1 has diversified roles in telomere protection and regulation in different organisms.

Chen Y et al. Nat Struct Mol Biol 2011 Feb;18(2):213-21

PMID:26063574 - Minishelterins separate telomere length regulation and end protection in fission yeast.

Pan L et al. Genes Dev 2015 Jun 01;29(11):1164-74

PMID:23133674 - Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe.

Fujita I et al. PLoS One 2012;7(11):e49151

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.

Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6

PMID:23861937 - Genome-wide screening for genes associated with valproic acid sensitivity in fission yeast.

Zhang L et al. PLoS One 2013;8(7):e68738

PMID:27334362 - Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions.

Tange Y et al. Genes Cells 2016 Aug;21(8):812-32

PMID:23188080 - The fission yeast MRN complex tethers dysfunctional telomeres for NHEJ repair.

Reis CC et al. EMBO J 2012 Dec 12;31(24):4576-86

PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.

Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32

PMID:27066066 - Rif1: A Conserved Regulator of DNA Replication and Repair Hijacked by Telomeres in Yeasts.

Mattarocci S et al. Front Genet 2016;7:45

PMID:11676925 - spRap1 and spRif1, recruited to telomeres by Taz1, are essential for telomere function in fission yeast.

Kanoh J et al. Curr Biol 2001 Oct 16;11(20):1624-30

PMID:23084836 - Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.

Tanaka A et al. Mol Cell 2012 Nov 30;48(4):532-46

PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.

Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134

PMID:22101932 - Tel1ATM and Rad3ATR kinases promote Ccq1-Est1 interaction to maintain telomeres in fission yeast.

Moser BA et al. Nat Struct Mol Biol 2011 Nov 20;18(12):1408-13

PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:29774234 - The fission yeast Stn1-Ten1 complex limits telomerase activity via its SUMO-interacting motif and promotes telomeres replication.

Matmati S et al. Sci Adv 2018 May;4(5):eaar2740

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:36059259 - Rap1 prevents fusions between long telomeres in fission yeast.

Pan L et al. EMBO J 2022 Oct 17;41(20):e110458

PMID:29422501 - LARP7 family proteins have conserved function in telomerase assembly.

Collopy LC et al. Nat Commun 2018 Feb 08;9(1):557

PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.

Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69

PMID:10759889 - Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library.

Ding DQ et al. Genes Cells 2000 Mar;5(3):169-90

PMID:24240238 - Elimination of shelterin components bypasses RNAi for pericentric heterochromatin assembly.

Tadeo X et al. Genes Dev 2013 Nov 15;27(22):2489-99

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).

Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:17429064 - Fission yeast Taz1 and RPA are synergistically required to prevent rapid telomere loss.

Kibe T et al. Mol Biol Cell 2007 Jun;18(6):2378-87

PMID:29422503 - LARP7-like protein Pof8 regulates telomerase assembly and poly(A)+TERRA expression in fission yeast.

Mennie AK et al. Nat Commun 2018 Feb 08;9(1):586

PMID:30503780 - The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope.

Hu C et al. Structure 2019 Feb 05;27(2):335-343.e3

PMID:19948484 - Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation.

Chikashige Y et al. J Cell Biol 2009 Nov 02;187(3):413-27

PMID:26058898 - Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation.

Amelina H et al. BMC Biol 2015 Jun 10;13:37

PMID:39833200 - Centromere positioning orchestrates telomere bouquet formation and the initiation of meiotic differentiation.

Jiménez-Martín A et al. Nat Commun 2025 Jan 20;16(1):837

PMID:31980821 - Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells.

Maestroni L et al. Nucleic Acids Res 2020 Apr 06;48(6):3029-3041

PMID:29997179 - Shelterin promotes tethering of late replication origins to telomeres for replication-timing control.

Ogawa S et al. EMBO J 2018 Aug 01;37(15)

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:35820914 - Antagonistic effects of mitochondrial matrix and intermembrane space proteases on yeast aging.

Vega M et al. BMC Biol 2022 Jul 12;20(1):160

PMID:36095128 - Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.

Anil AT et al. Nucleic Acids Res 2022 Sep 23;50(17):10000-10014

PMID:20625380 - A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length.

Liu NN et al. Cell Res 2010 Aug;20(8):963-5

PMID:9572142 - Defective meiosis in telomere-silencing mutants of Schizosaccharomyces pombe.

Nimmo ER et al. Nature 1998 Apr 23;392(6678):825-8

PMID:27889481 - Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.

Fernández-Álvarez A et al. Dev Cell 2016 Dec 05;39(5):544-559

PMID:29216371 - Fission yeast Ccq1 is a modulator of telomerase activity.

Armstrong CA et al. Nucleic Acids Res 2018 Jan 25;46(2):704-716

PMID:11676924 - Telomere binding of the Rap1 protein is required for meiosis in fission yeast.

Chikashige Y et al. Curr Biol 2001 Oct 16;11(20):1618-23

PMID:19443688 - Diverse roles of HP1 proteins in heterochromatin assembly and functions in fission yeast.

Fischer T et al. Proc Natl Acad Sci U S A 2009 Jun 02;106(22):8998-9003

PMID:17632059 - The telomere bouquet controls the meiotic spindle.

Tomita K et al. Cell 2007 Jul 13;130(1):113-26

PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.

Escandell JM et al. EMBO J 2019 Apr 01;38(7)

PMID:31289327 - Telomere DNA length-dependent regulation of DNA replication timing at internal late replication origins.

Hasegawa Y et al. Sci Rep 2019 Jul 09;9(1):9946

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.

Blyth J et al. Genetics 2018 Feb;208(2):589-603

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:12018857 - Fission yeast Rap1 homolog is a telomere-specific silencing factor and interacts with Taz1p.

Park MJ et al. Mol Cells 2002 Apr 30;13(2):327-33

PMID:23173672 - Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.

Pan X et al. BMC Genomics 2012 Nov 23;13:662

PMID:24925530 - Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association.

Garg M et al. EMBO Rep 2014 Aug;15(8):871-7

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:25131669 - Telomerase activation after recruitment in fission yeast.

Armstrong CA et al. Curr Biol 2014 Sep 08;24(17):2006-11

PMID:32062975 - Phosphoproteomics Reveals Novel Targets and Phosphoprotein Networks in Cell Cycle Mediated by Dsk1 Kinase.

Wu M et al. J Proteome Res 2020 Apr 03;19(4):1776-1787

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.

Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15

PMID:25778919 - Aurora B prevents chromosome arm separation defects by promoting telomere dispersion and disjunction.

Reyes C et al. J Cell Biol 2015 Mar 16;208(6):713-27

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:29160296 - Structure of the fission yeast S. pombe telomeric Tpz1-Poz1-Rap1 complex.

Xue J et al. Cell Res 2017 Dec;27(12):1503-1520

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8