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protein coding gene - arc3 (SPBC1778.08c) - ARP2/3 actin-organizing complex subunit Arc21

Gene summary

Standard name
arc3
Systematic ID
SPBC1778.08c
Product
ARP2/3 actin-organizing complex subunit Arc21
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
arc21, arc18
UniProt ID
Q9Y7J4
ORFeome ID
09/09B02
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3111115..3112237 reverse strand

Annotation

Complementation

PBO:0009256 - functionally complemented by human ARPC3

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GO biological process

GO:0000147 - actin cortical patch assembly

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GO:0090135 - actin filament branching

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GO:0034314 - Arp2/3 complex-mediated actin nucleation

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GO:0072583 - clathrin-dependent endocytosis

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GO:0007163 - establishment or maintenance of cell polarity

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GO cellular component

GO:0030479 - actin cortical patch

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GO:0005885 - Arp2/3 protein complex

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GO:0032153 - cell division site

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GO:0051286 - cell tip

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GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0032432 - actin filament bundle

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GO:0005826 - actomyosin contractile ring

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GO molecular function

GO:0051015 - actin filament binding

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Modification

MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000018 - protein level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000080 - decreased cell population growth at low temperature

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Genotypes:

FYPO:0000316 - inviable after spore germination

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Genotypes:

FYPO:0002427 - inviable spore with abnormal shape

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04062P21-ArcARPC3Pfam
G3DSA:1.10.1760.10ARPC3_sfCATH-Gene3D
SSF69060ARPC3_sfSUPERFAMILY
PTHR12391ARPC3PANTHER
PIRSF016315ARP2/3_P21-ArcARPC3PIRSF

Orthologs

References / Literature

PMID:32839613 - Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
Shaaban M et al. Nat Struct Mol Biol 2020 Nov;27(11):1009-1016
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:16087707 - Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast.
Sirotkin V et al. J Cell Biol 2005 Aug 15;170(4):637-48
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:19362000 - Cofilin dissociates Arp2/3 complex and branches from actin filaments.
Chan C et al. Curr Biol 2009 Apr 14;19(7):537-45
PMID:18640983 - Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Nolen BJ et al. J Biol Chem 2008 Sep 26;283(39):26490-8
PMID:21449051 - Schizosaccharomyces pombe Arc3 is a conserved subunit of the Arp2/3 complex required for polarity, actin organization, and endocytosis.
Cabrera R et al. Yeast 2011 Jun;28(6):495-503
PMID:36442092 - Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
Chou SZ et al. Proc Natl Acad Sci U S A 2022 Dec 06;119(49):e2206722119
PMID:10588653 - A mutant of Arp2p causes partial disassembly of the Arp2/3 complex and loss of cortical actin function in fission yeast.
Morrell JL et al. Mol Biol Cell 1999 Dec;10(12):4201-15
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20668161 - Proteasome nuclear import mediated by Arc3 can influence efficient DNA damage repair and mitosis in Schizosaccharomyces pombe.
Cabrera R et al. Mol Biol Cell 2010 Sep 15;21(18):3125-36
PMID:16224022 - Counting cytokinesis proteins globally and locally in fission yeast.
Wu JQ et al. Science 2005 Oct 14;310(5746):310-4
PMID:21642440 - Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.
Skau CT et al. J Biol Chem 2011 Jul 29;286(30):26964-77
PMID:30150414 - Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.
Espinoza-Sanchez S et al. Proc Natl Acad Sci U S A 2018 Sep 11;115(37):E8642-E8651
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:38448439 - Cryo-EM structures reveal how phosphate release from Arp3 weakens actin filament branches formed by Arp2/3 complex.
Chavali SS et al. Nat Commun 2024 Mar 06;15(1):2059
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:18165685 - Pathway of actin filament branch formation by Arp2/3 complex.
Beltzner CC et al. J Biol Chem 2008 Mar 14;283(11):7135-44