protein coding gene - rar1 (SPBC17A3.04c) - cytoplasmic methionine-tRNA ligase Mrs1

Gene summary

Standard name: rar1
Systematic ID: SPBC17A3.04c
Product: cytoplasmic methionine-tRNA ligase Mrs1
Organism: Schizosaccharomyces pombe (fission yeast)
Synonyms: mrs1, mes1, pi042
UniProt ID: Q9UUF2
ORFeome ID: 40/40H09
Characterisation status: biological role inferred
Feature type: mRNA gene
Genomic location: chromosome II: 1406917..1409883 reverse strand

Annotation

PBO:0002323 - 6.1.1.10

Disease association

MONDO:0018422 - autosomal recessive spastic paraplegia type 70

References:

PB_REF:0000006

MONDO:0014566 - Charcot-Marie-Tooth disease axonal type 2U

References:

PB_REF:0000006

MONDO:0014206 - severe early-onset pulmonary alveolar proteinosis due to MARS deficiency

References:

PB_REF:0000006

MONDO:0030518 - trichothiodystrophy 9, nonphotosensitive

References:

PB_REF:0000006

GO biological process

GO:0002181 - cytoplasmic translation

References:

GO_REF:0000051

GO cellular component

GO:0017101 - aminoacyl-tRNA synthetase multienzyme complex

References:

GO_REF:0000033

GO:0010494 - cytoplasmic stress granule

References:

PMID:32071154

GO:0005829 - cytosol

References:

PMID:16823372

GO:0017102 - methionyl glutamyl tRNA synthetase complex

References:

PMID:31285271

GO molecular function

GO:0005524 - ATP binding

References:

GO_REF:0000002

GO:0004825 - methionine-tRNA ligase activity

References:

GO_REF:0000033

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

References:

PMID:27984744

PomGeneEx:0000011 - RNA level increased

References:

PMID:16537923

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

PMID:23101633

Single locus phenotype

FYPO:0000311 - inviable after spore germination with normal, unseptated germ tube morphology

References:

PMID:23697806

Genotypes:

rar1delta

FYPO:0002151 - inviable spore

References:

PMID:23697806

Genotypes:

rar1delta

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

rar1delta

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0001338 - gene name conflict

Protein features

ID	Name	InterPro name	DB name
PF09334	tRNA-synt_1g	Methionyl/Leucyl_tRNA_Synth	Pfam
PF19303	Anticodon_3	Anticodon_Met	Pfam
cd00814	MetRS_core	MetRS_core	CDD
cd07957	Anticodon_Ia_Met	Anticodon_Met	CDD
PS00178	AA_TRNA_LIGASE_I	aa-tRNA-synth_I_CS	PROSITE patterns
PR01041	TRNASYNTHMET	MetRS_core	PRINTS
G3DSA:1.10.730.10:FF:000037			CATH-FunFam
G3DSA:2.20.28.20:FF:000001			CATH-FunFam
G3DSA:1.10.730.10			CATH-Gene3D
G3DSA:2.20.28.20		MetRS_Zn	CATH-Gene3D
G3DSA:3.40.50.620		Rossmann-like_a/b/a_fold	CATH-Gene3D
SSF47323		tRNAsynth_Ia_anticodon-bd	SUPERFAMILY
SSF52374			SUPERFAMILY
SSF57770		MetRS_Zn	SUPERFAMILY
PTHR45765		Met-tRNA_ligase_1	PANTHER
TIGR00398	metG	Met-tRNA_synth	NCBIFAM
MF_00098	Met_tRNA_synth_type1	Met-tRNA_ligase_1	HAMAP

Orthologs

MARS1 (HGNC:6898) Homo sapiens
rar1 (SJAG_01159) Schizosaccharomyces japonicus
MES1 (YGR264C) Saccharomyces cerevisiae

References / Literature

PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).: Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.: Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.: Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.: Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.: Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.: Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.: Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:21511999 - Comparative functional genomics of the fission yeasts.: Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.: Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:31285271 - Fission Yeast Asc1 Stabilizes the Interaction between Eukaryotic Initiation Factor 3a and Rps0A/uS2 for Protein Synthesis.: Wang YT et al. Mol Cell Biol 2019 Oct 01;39(19)
PMID:34496258 - Transcription and chromatin-based surveillance mechanism controls suppression of cryptic antisense transcription.: Heo DH et al. Cell Rep 2021 Sep 07;36(10):109671
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.: Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:21652630 - Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis.: Snaith HA et al. J Cell Sci 2011 Jul 01;124(Pt 13):2187-99
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.: Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
GO_REF:0000051 - S. pombe keyword mapping
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.: Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.: Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.: Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.: Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:32071154 - Fission Yeast Puf2, a Pumilio and FBF Family RNA-Binding Protein, Links Stress Granules to Processing Bodies.: Hsiao WY et al. Mol Cell Biol 2020 Apr 13;40(9)
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.: Skribbe M et al. Mol Cell 2025 Feb 19;
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000002 - Comments
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.: Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.: Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41