PomBase home

protein coding gene - spp2 (SPBC17D11.06) - DNA primase large subunit Spp2

Gene summary

Standard name
spp2
Systematic ID
SPBC17D11.06
Product
DNA primase large subunit Spp2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
pri2
UniProt ID
O74761
ORFeome ID
35/35B07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3317773..3319629 forward strand

Annotation

GO biological process

GO:0006270 - DNA replication initiation

References:

GO:0006269 - DNA replication, synthesis of primer

References:

GO cellular component

GO:0005658 - alpha DNA polymerase:primase complex

References:

GO:0005737 - cytoplasm

References:

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003899 - DNA-directed RNA polymerase activity

References:

GO:0003697 - single-stranded DNA binding

References:

Multi-locus phenotype

FYPO:0001430 - abnormal mitotic cell cycle arrest with unreplicated DNA

References:

Genotypes:

FYPO:0000062 - abnormal nuclear morphology during vegetative growth

References:

Genotypes:

FYPO:0006728 - abolished mitotic DNA replication initiation

References:

Genotypes:

FYPO:0005095 - abolished protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0006731 - decreased CMG complex progression from replication origin

References:

Genotypes:

FYPO:0006729 - decreased protein localization to chromatin distal to replication origin

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0004961 - increased protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0004962 - normal protein localization to chromatin at replication origin

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0006822 - viable small vegetative cell with normal cell growth rate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0003165 - cut with abnormal chromosome segregation

References:

Genotypes:

FYPO:0004371 - decreased duration of S-phase DNA damage checkpoint

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0002097 - decreased protein kinase activity during cellular response to hydroxyurea

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000453 - DNA content decreased during vegetative growth

References:

Genotypes:

FYPO:0000158 - DNA content increased during vegetative growth

References:

Genotypes:

FYPO:0002019 - elongated telomeres during vegetative growth

References:

Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0001042 - inviable after spore germination, single or double cell division

References:

Genotypes:

FYPO:0001924 - inviable after spore germination, without cell division, cell cycle arrest

References:

Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

References:

Genotypes:

FYPO:0004255 - inviable elongated mononucleate vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0000333 - mitotic G1/S phase transition delay

References:

Genotypes:

FYPO:0003166 - monoseptate vegetative cell with binucleate and anucleate compartments

References:

Genotypes:

FYPO:0003555 - normal chromatin silencing at subtelomere

References:

Genotypes:

FYPO:0003353 - normal DNA double-strand break formation at mating-type locus

References:

Genotypes:

FYPO:0000969 - normal growth during cellular response to UV

References:

Genotypes:

FYPO:0000963 - normal growth on hydroxyurea

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0000776 - normal protein phosphorylation during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF04104DNA_primase_lrgDNA_primase_CPfam
PF26466DNA_primase_lrg_NPfam
cd07322PriL_PriS_EukaryoticDNA_primase_lsu_eukCDD
G3DSA:1.20.930.80:FF:000003CATH-FunFam
G3DSA:1.20.930.80CATH-Gene3D
SSF140914SUPERFAMILY
PTHR10537DNA_primase_lsu_euk/arcPANTHER
PIRSF009449DNA_primase_large_subunitDNA_primase_lsu_eukPIRSF

Orthologs

References / Literature

GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:11027257 - Analysis of fission yeast primase defines the checkpoint responses to aberrant S phase initiation.
Tan S et al. Mol Cell Biol 2000 Nov;20(21):7853-66
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30796050 - Ssu72 phosphatase is a conserved telomere replication terminator.
Escandell JM et al. EMBO J 2019 Apr 01;38(7)
PMID:11160827 - Role of fission yeast primase catalytic subunit in the replication checkpoint.
Griffiths DJ et al. Mol Biol Cell 2001 Jan;12(1):115-28
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:12697806 - Replication proteins influence the maintenance of telomere length and telomerase protein stability.
Dahlén M et al. Mol Cell Biol 2003 May;23(9):3031-42
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
PMID:15314153 - The B-subunit of DNA polymerase alpha-primase associates with the origin recognition complex for initiation of DNA replication.
Uchiyama M et al. Mol Cell Biol 2004 Sep;24(17):7419-34
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053