protein coding gene - cnp3 (SPBC1861.01c) - CENP-C ortholog, kinetochore scaffold Cnp3

Gene summary

Standard name

cnp3

Systematic ID

SPBC1861.01c

Product

CENP-C ortholog, kinetochore scaffold Cnp3

Organism

Schizosaccharomyces pombe (fission yeast)

Synonyms

SPBC56F2.13, mif2

UniProt ID

Q9USR9

ORFeome ID

37/37H08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 4126318..4128666 reverse strand

Annotation

Protein features

Orthologs

• CENPC (HGNC:1854) Homo sapiens
• cnp3 (SJAG_03217) Schizosaccharomyces japonicus
• MIF2 (YKL089W) Saccharomyces cerevisiae

References / Literature

PMID:28904333 - The Ino80 complex mediates epigenetic centromere propagation via active removal of histone H3.

Choi ES et al. Nat Commun 2017 Sep 13;8(1):529

PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.

Jian Y et al. J Cell Sci 2023 Jan 15;136(2)

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:25619765 - The CENP-A N-tail confers epigenetic stability to centromeres via the CENP-T branch of the CCAN in fission yeast.

Folco HD et al. Curr Biol 2015 Feb 02;25(3):348-356

PMID:38133430 - Genetic suppressor screen identifies Tgp1 (glycerophosphocholine transporter), Kcs1 (IP 6 kinase), and Plc1 (phospholipase C) as determinants of inositol pyrophosphate toxicosis in fission yeast.

Bednor L et al. mBio 2023 Dec 22;:e0306223

PMID:16325576 - The kinetochore protein Moa1 enables cohesion-mediated monopolar attachment at meiosis I.

Yokobayashi S et al. Cell 2005 Dec 02;123(5):803-17

PMID:15791413 - Comparison of Dam tagging and chromatin immunoprecipitation as tools for the identification of the binding sites for S. pombe CENP-C.

Holland S et al. Chromosome Res 2005;13(1):73-83

PMID:23028377 - Factors that promote H3 chromatin integrity during transcription prevent promiscuous deposition of CENP-A(Cnp1) in fission yeast.

Choi ES et al. PLoS Genet 2012 Sep;8(9):e1002985

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:29925533 - Fission Yeast CENP-C (Cnp3) Plays a Role in Restricting the Site of CENP-A Accumulation.

Suma M et al. G3 (Bethesda) 2018 Jul 31;8(8):2723-2733

PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.

Halova L et al. Open Biol 2021 Apr;11(4):200405

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.

Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567

PMID:29180432 - Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.

Zhou X et al. Proc Natl Acad Sci U S A 2017 Dec 12;114(50):E10667-E10676

PMID:17627824 - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.

Gregan J et al. Curr Biol 2007 Jul 17;17(14):1190-200

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7

PMID:33711009 - Structure-function analysis of fission yeast cleavage and polyadenylation factor (CPF) subunit Ppn1 and its interactions with Dis2 and Swd22.

Benjamin B et al. PLoS Genet 2021 Mar;17(3):e1009452

PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.

Køhler JB et al. Nat Commun 2015 Nov 05;6:8827

PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.

Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160

PMID:34849791 - Perturbation of kinetochore function using GFP-binding protein in fission yeast.

Deng DJ et al. G3 (Bethesda) 2021 Oct 19;11(11)

PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.

Skribbe M et al. Mol Cell 2025 Feb 19;

PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.

Maki T et al. PLoS Genet 2018 May;14(5):e1007424

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.

Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77

PMID:24327658 - Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis.

Brookheart RT et al. J Biol Chem 2014 Jan 31;289(5):2725-35

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:26275423 - Inner Kinetochore Protein Interactions with Regional Centromeres of Fission Yeast.

Thakur J et al. Genetics 2015 Oct;201(2):543-61

PMID:28977643 - Regulation of mitotic recombination between DNA repeats in centromeres.

Zafar F et al. Nucleic Acids Res 2017 Nov 02;45(19):11222-11235

PMID:31366733 - Structures of CENP-C cupin domains at regional centromeres reveal unique patterns of dimerization and recruitment functions for the inner pocket.

Chik JK et al. J Biol Chem 2019 Sep 20;294(38):14119-14134

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

GO_REF:0000033 - Annotation inferences using phylogenetic trees

PMID:38084929 - The cysteine-rich domain in CENP-A chaperone Scm3HJURP ensures centromere targeting and kinetochore integrity.

Folco HD et al. Nucleic Acids Res 2023 Dec 12;

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:23267073 - Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.

Gonzalez M et al. Proc Natl Acad Sci U S A 2013 Jan 08;110(2):606-11

PMID:21633354 - Condensin association with histone H2A shapes mitotic chromosomes.

Tada K et al. Nature 2011 Jun 01;474(7352):477-83

PMID:23936074 - Telomeric repeats facilitate CENP-A(Cnp1) incorporation via telomere binding proteins.

Castillo AG et al. PLoS One 2013;8(7):e69673

PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.

Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928

PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.

Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43

PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.

Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84

PMID:20723757 - The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments.

Corbett KD et al. Cell 2010 Aug 20;142(4):556-67

PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.

Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399