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protein coding gene - sim4 (SPBC18E5.03c) - CENP-K ortholog Sim4

Gene summary

Standard name
sim4
Systematic ID
SPBC18E5.03c
Product
CENP-K ortholog Sim4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94494
ORFeome ID
12/12F01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2079136..2080852 reverse strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

References:

GO:0000070 - mitotic sister chromatid segregation

References:

GO cellular component

GO:0000779 - condensed chromosome, centromeric region

References:

GO:0005829 - cytosol

References:

GO:0000939 - inner kinetochore

References:

GO:0031511 - Mis6-Sim4 complex

References:

GO:0005634 - nucleus

References:

Qualitative gene expression

PomGeneEx:0000012 - RNA level decreased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0004308 - abnormal CENP-A containing chromatin organization

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0004318 - abolished mitotic spindle assembly checkpoint

References:

Genotypes:

FYPO:0003786 - abolished protein localization to centromere central core during vegetative growth

References:

Genotypes:

FYPO:0001270 - complete but unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

References:

Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0002649 - elongated kinetochore during mitosis

References:

Genotypes:

FYPO:0005096 - increased duration of protein localization to mitotic spindle pole body during anaphase

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000228 - lagging mitotic chromosomes

References:

Genotypes:

FYPO:0004307 - long mitotic spindle during metaphase

References:

Genotypes:

FYPO:0002901 - normal protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0005216 - normal protein localization to mitotic spindle pole body during metaphase

References:

Genotypes:

FYPO:0000091 - sensitive to thiabendazole

References:

Genotypes:

FYPO:0000732 - short bipolar mitotic spindle

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF11802CENP-KCentromere_CenpKPfam
PTHR14401Centromere_CenpKPANTHER
CoilCOILS

Orthologs

References / Literature

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.
Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77
PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.
Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78
PMID:17035632 - Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
Hayashi A et al. Mol Biol Cell 2006 Dec;17(12):5173-84
PMID:17627824 - The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.
Gregan J et al. Curr Biol 2007 Jul 17;17(14):1190-200
PMID:19758558 - CENP-C functions as a scaffold for effectors with essential kinetochore functions in mitosis and meiosis.
Tanaka K et al. Dev Cell 2009 Sep;17(3):334-43
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.
Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:29899117 - Ccp1 modulates epigenetic stability at centromeres and affects heterochromatin distribution in Schizosaccharomyces pombe .
Lu M et al. J Biol Chem 2018 Aug 03;293(31):12068-12080
PMID:31474649 - Identification of 15 New Bypassable Essential Genes of Fission Yeast.
Takeda A et al. Cell Struct Funct 2019 Sep 27;44(2):113-119
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.
Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928
PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.
Jian Y et al. J Cell Sci 2023 Jan 15;136(2)
PMID:38084929 - The cysteine-rich domain in CENP-A chaperone Scm3HJURP ensures centromere targeting and kinetochore integrity.
Folco HD et al. Nucleic Acids Res 2023 Dec 12;
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:40015273 - A comprehensive Schizosaccharomyces pombe atlas of physical transcription factor interactions with proteins and chromatin.
Skribbe M et al. Mol Cell 2025 Feb 19;
PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.
Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54
PMID:21445296 - Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.
Shiroiwa Y et al. PLoS One 2011 Mar 21;6(3):e17761
PMID:22561346 - CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Schleiffer A et al. Nat Cell Biol 2012 May 06;14(6):604-13
PMID:19217404 - Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
Pidoux AL et al. Mol Cell 2009 Feb 13;33(3):299-311