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protein coding gene - ape2 (SPBC1921.05) - vacuolar aminopeptidase Ape2

Gene summary

Standard name
ape2
Systematic ID
SPBC1921.05
Product
vacuolar aminopeptidase Ape2
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
tma108, ape1
UniProt ID
Q9USX1
ORFeome ID
38/38G07
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2412978..2416541 forward strand

Annotation

PBO:0002712 - 3.4.11.-

GO biological process

GO:0120113 - cytoplasm to vacuole targeting by the NVT pathway

References:

GO:0043171 - peptide catabolic process

References:

GO:0016485 - protein processing

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GO cellular component

GO:0005829 - cytosol

References:

GO:0005576 - extracellular region

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GO:0000328 - fungal-type vacuole lumen

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GO:0005771 - multivesicular body

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GO:0061957 - NVT complex

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GO molecular function

GO:0030145 - manganese ion binding

References:

GO:0070006 - metalloaminopeptidase activity

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GO:0008270 - zinc ion binding

References:

Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0000021 - spheroid vegetative cell

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Genotypes:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0006274 - abolished protein localization via NVT pathway

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0006294 - normal macroautophagy during nitrogen starvation

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Genotypes:

FYPO:0002896 - normal transcription during cellular response to calcium ion

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Genotypes:

FYPO:0001315 - normal vegetative cell morphology

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0001583 - resistance to lithium

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Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000799 - sensitive to diamide

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

Protein features

IDNameInterPro nameDB name
PF01433Peptidase_M1Peptidase_M1_domPfam
PF11838ERAP1_CERAP1-like_C_domPfam
PF17900Peptidase_M1_NAminopeptidase_N-like_NPfam
cd09601M1_APN-Q_likeM1_APN-typCDD
PR00756ALADIPTASEPeptidase_M1PRINTS
G3DSA:1.10.390.10:FF:000001CATH-FunFam
G3DSA:1.25.50.20:FF:000002CATH-FunFam
G3DSA:2.60.40.1730:FF:000002CATH-FunFam
G3DSA:2.60.40.1910:FF:000004CATH-FunFam
G3DSA:1.10.390.10Peptidase_M4/M1_CTD_sfCATH-Gene3D
G3DSA:1.25.50.20CATH-Gene3D
G3DSA:2.60.40.1730Aminopeptidase_N-like_N_sfCATH-Gene3D
G3DSA:2.60.40.1910CATH-Gene3D
SSF55486SUPERFAMILY
SSF63737Aminopeptidase_N-like_N_sfSUPERFAMILY
PTHR11533Peptidase_M1_aminopeptidasesPANTHER

Orthologs

References / Literature

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:34083046 - Function and molecular mechanisms of APE2 in genome and epigenome integrity.
Lin Y et al. Mutat Res Rev Mutat Res 2021;787:108347
PMID:34169534 - Molecular and structural mechanisms of ZZ domain-mediated cargo selection by Nbr1.
Wang YY et al. EMBO J 2021 Aug 02;40(15):e107497
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.
Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24192486 - The conserved Fanconi anemia nuclease Fan1 and the SUMO E3 ligase Pli1 act in two novel Pso2-independent pathways of DNA interstrand crosslink repair in yeast.
Fontebasso Y et al. DNA Repair (Amst) 2013 Dec;12(12):1011-23
PMID:21504829 - Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex.
Stewart EV et al. Mol Cell 2011 Apr 22;42(2):160-71
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:34805795 - The fission yeast FLCN/FNIP complex augments TORC1 repression or activation in response to amino acid (AA) availability.
Calvo IA et al. iScience 2021 Nov 19;24(11):103338
PMID:16491466 - Construction of a protease-deficient strain set for the fission yeast Schizosaccharomyces pombe, useful for effective production of protease-sensitive heterologous proteins.
Idiris A et al. Yeast 2006 Jan 30;23(2):83-99
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:17426133 - The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair.
Gembka A et al. Nucleic Acids Res 2007;35(8):2596-608
PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:26365378 - ESCRTs Cooperate with a Selective Autophagy Receptor to Mediate Vacuolar Targeting of Soluble Cargos.
Liu XM et al. Mol Cell 2015 Sep 17;59(6):1035-42
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
GO_REF:0000051 - S. pombe keyword mapping
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:22496451 - Genetic screening for regulators of Prz1, a transcriptional factor acting downstream of calcineurin in fission yeast.
Koike A et al. J Biol Chem 2012 Jun 01;287(23):19294-303