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protein coding gene - bqt4 (SPBC19C7.10) - integral nuclear inner membrane protein Bqt4

Gene summary

Standard name
bqt4
Systematic ID
SPBC19C7.10
Product
integral nuclear inner membrane protein Bqt4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O60158
ORFeome ID
45/45A08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2840834..2842834 forward strand

Annotation

GO biological process

GO:0070197 - meiotic attachment of telomere to nuclear envelope

References:

GO:0045141 - meiotic telomere clustering

References:

GO:0044821 - meiotic telomere tethering at nuclear periphery

References:

GO:0044820 - mitotic telomere tethering at nuclear periphery

References:

GO:0071763 - nuclear membrane organization

References:

GO:0000723 - telomere maintenance

References:

GO:0032200 - telomere organization

References:

GO cellular component

GO:0016020 - membrane

References:

GO:0005635 - nuclear envelope

References:

GO:0005637 - nuclear inner membrane

References:

GO:0031965 - nuclear membrane

References:

GO:1990862 - nuclear membrane complex Bqt3-Bqt4

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0003677 - DNA binding

References:

GO:0070300 - phosphatidic acid binding

References:

GO:0005515 - protein binding

References:

GO:0140473 - telomere-nuclear envelope anchor activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

MOD:00048 - O4'-phospho-L-tyrosine

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000769 - abnormal nuclear envelope morphology during vegetative growth

References:

Genotypes:

FYPO:0004790 - abnormal telomere-nuclear envelope distance during vegetative growth

References:

Genotypes:

FYPO:0002568 - abolished protein localization to nuclear periphery

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0000770 - branched nuclear inner membrane

References:

Genotypes:

FYPO:0001053 - cut, normal size cell

References:

Genotypes:

FYPO:0006881 - decreased cellular phytoceramide level

References:

Genotypes:

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0000940 - decreased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0007419 - decreased telomere tethering at nuclear periphery during G0

References:

Genotypes:

FYPO:0004085 - decreased vegetative cell growth

References:

Genotypes:

FYPO:0006882 - imperforate nuclear envelope

References:

Genotypes:

FYPO:0005407 - increased DNA recombination at telomere during nitrogen starvation

References:

Genotypes:

FYPO:0001571 - increased protein-protein interaction

References:

Genotypes:

FYPO:0004890 - increased telomere-nuclear envelope distance during mitosis

References:

Genotypes:

FYPO:0007421 - increased transcription at telomere during G0

References:

Genotypes:

FYPO:0002908 - increased transcription at telomere during vegetative growth

References:

Genotypes:

FYPO:0002430 - inviable after spore germination, multiple cell divisions

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0001839 - normal minichromosome loss

References:

Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0000772 - perforated nuclear envelope

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0006511 - shortened telomeres during G0

References:

Genotypes:

FYPO:0007414 - subtelomere expansion during G0

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Protein features

PBO:0111791 - APSES domain

Protein sequence feature

SO:0001534 - nuclear_rim_localization_signal

References:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

PomGeneEx:0000014 - RNA present

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000172 - abnormal meiotic telomere clustering

References:

Genotypes:

FYPO:0000769 - abnormal nuclear envelope morphology during vegetative growth

References:

Genotypes:

FYPO:0004885 - abnormal protein localization to nuclear envelope during vegetative growth

References:

Genotypes:

FYPO:0002338 - abnormal protein localization to nuclear periphery

References:

Genotypes:

FYPO:0000117 - abnormal septum assembly

References:

Genotypes:

FYPO:0001894 - abnormal sporulation resulting in formation of ascus with more or fewer than four spores

References:

Genotypes:

FYPO:0004790 - abnormal telomere-nuclear envelope distance during vegetative growth

References:

Genotypes:

FYPO:0006366 - abolished meiotic telomere clustering

References:

Genotypes:

FYPO:0004886 - abolished protein localization to nuclear envelope during vegetative growth

References:

Genotypes:

FYPO:0004889 - abolished protein localization to telomere during meiotic cell cycle

References:

Genotypes:

FYPO:0000705 - abolished protein-protein interaction

References:

Genotypes:

FYPO:0006692 - cut with septum between unequally sized nuclei

References:

Genotypes:

FYPO:0000046 - decreased cell population growth

References:

Genotypes:

FYPO:0006800 - decreased centromere clustering at nuclear periphery during mitotic interphase

References:

Genotypes:

FYPO:0002834 - decreased chromatin silencing at centromere

References:

Genotypes:

FYPO:0002827 - decreased chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0000658 - decreased DNA binding

References:

Genotypes:

FYPO:0002485 - decreased intergenic meiotic recombination

References:

Genotypes:

FYPO:0005915 - decreased nucleosome occupancy at 5S rRNA genes

References:

Genotypes:

FYPO:0005932 - decreased nucleosome occupancy at long terminal repeat

References:

Genotypes:

FYPO:0005916 - decreased nucleosome occupancy at tRNA genes

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0007073 - decreased protein localization to nuclear envelope during vegetative growth

References:

Genotypes:

FYPO:0002339 - decreased protein localization to nuclear periphery

References:

Genotypes:

FYPO:0001645 - decreased protein-protein interaction

References:

Genotypes:

FYPO:0000581 - decreased spore germination frequency

References:

Genotypes:

FYPO:0007419 - decreased telomere tethering at nuclear periphery during G0

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0001556 - excess nuclear envelope present

References:

Genotypes:

FYPO:0007457 - excess nuclear envelope present during mitotic interphase

References:

Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

References:

Genotypes:

FYPO:0008433 - increased inner nuclear membrane phosphatidic acid level

References:

Genotypes:

FYPO:0005371 - increased linear minichromosome loss during vegetative growth

References:

Genotypes:

FYPO:0002969 - increased protein localization to mitotic spindle pole body

References:

Genotypes:

FYPO:0004890 - increased telomere-nuclear envelope distance during mitosis

References:

Genotypes:

FYPO:0004791 - increased telomere-nuclear envelope distance during mitotic G2 phase

References:

Genotypes:

FYPO:0007421 - increased transcription at telomere during G0

References:

Genotypes:

FYPO:0002908 - increased transcription at telomere during vegetative growth

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0006518 - loss of viability in G0

References:

Genotypes:

FYPO:0001870 - normal centromere clustering at nuclear periphery during vegetative growth

References:

Genotypes:

FYPO:0002360 - normal chromatin silencing at centromere

References:

Genotypes:

FYPO:0002336 - normal chromatin silencing at silent mating-type cassette

References:

Genotypes:

FYPO:0007553 - normal G1 to G0 transition

References:

Genotypes:

FYPO:0004093 - normal meiotic telomere clustering

References:

Genotypes:

FYPO:0004083 - normal protein level

References:

Genotypes:

FYPO:0000833 - normal protein level during vegetative growth

References:

Genotypes:

FYPO:0003627 - normal protein localization

References:

Genotypes:

FYPO:0005612 - normal protein localization to nuclear envelope

References:

Genotypes:

FYPO:0004887 - normal protein localization to nuclear envelope during vegetative growth

References:

Genotypes:

FYPO:0002563 - normal protein localization to nuclear periphery

References:

Genotypes:

FYPO:0000703 - normal protein-protein interaction

References:

Genotypes:

FYPO:0001317 - normal RNA level during vegetative growth

References:

Genotypes:

FYPO:0000590 - normal sporulation

References:

Genotypes:

FYPO:0006515 - normal telomere length

References:

Genotypes:

FYPO:0006516 - normal telomere length during G0

References:

Genotypes:

FYPO:0002687 - normal telomere length during vegetative growth

References:

Genotypes:

FYPO:0004924 - normal telomere tethering at nuclear periphery during vegetative growth

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0008431 - nuclear lipid droplet formation

References:

Genotypes:

FYPO:0000772 - perforated nuclear envelope

References:

Genotypes:

FYPO:0000764 - resistance to cycloheximide

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0001034 - resistance to tunicamycin

References:

Genotypes:

FYPO:0001701 - sensitive to bortezomib

References:

Genotypes:

FYPO:0000799 - sensitive to diamide

References:

Genotypes:

FYPO:0007931 - sensitive to egtazic acid

References:

Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

References:

Genotypes:

FYPO:0000785 - sensitive to formamide

References:

Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009084 - sensitive to lithium chloride and methyl methanesulfonate

References:

Genotypes:

FYPO:0000089 - sensitive to methyl methanesulfonate

References:

Genotypes:

FYPO:0001492 - viable elongated vegetative cell

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0000055 - no apparent S. cerevisiae ortholog

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PS51299HTH_APSESTscrpt_reg_HTH_APSES-typePROSITE_PROFILES
SM01252KilA_N_2KilA/APSES_HTHSMART
SSF54616DNA-binding domain of Mlu1-box binding protein MBP1HTH_APSES_sfSUPERFAMILY
G3DSA:3.10.260.10HTH_APSES_sfGENE3D
G3DSA:1.20.120.20ApolipoproteinGENE3D
PTHR38044BOUQUET FORMATION PROTEIN 4Bqt4PANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:19948484 - Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation.
Chikashige Y et al. J Cell Biol 2009 Nov 02;187(3):413-27
PMID:31131414 - Casein kinase 2 regulates telomere protein complex formation through Rap1 phosphorylation.
Inoue H et al. Nucleic Acids Res 2019 Jul 26;47(13):6871-6884
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
GO_REF:0000002 - Comments
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:23133674 - Identification of the functional domains of the telomere protein Rap1 in Schizosaccharomyces pombe.
Fujita I et al. PLoS One 2012;7(11):e49151
PMID:23084836 - Epigenetic regulation of condensin-mediated genome organization during the cell cycle and upon DNA damage through histone H3 lysine 56 acetylation.
Tanaka A et al. Mol Cell 2012 Nov 30;48(4):532-46
PMID:30462301 - Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.
Hu C et al. Nucleic Acids Res 2019 Feb 20;47(3):1573-1584
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:29722648 - Distinct 'safe zones' at the nuclear envelope ensure robust replication of heterochromatic chromosome regions.
Ebrahimi H et al. Elife 2018 May 03;7
PMID:32817556 - Integrity of a heterochromatic domain ensured by its boundary elements.
Charlton SJ et al. Proc Natl Acad Sci U S A 2020 Sep 01;117(35):21504-21511
PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.
Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)
PMID:37694715 - A ubiquitin-proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis.
Le TK et al. J Cell Sci 2023 Oct 01;136(19)
PMID:33109728 - High-Throughput Identification of Nuclear Envelope Protein Interactions in Schizosaccharomyces pombe Using an Arrayed Membrane Yeast-Two Hybrid Library.
Varberg JM et al. G3 (Bethesda) 2020 Dec 03;10(12):4649-4663
PMID:26058898 - Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation.
Amelina H et al. BMC Biol 2015 Jun 10;13:37
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:38825008 - Disordered region of nuclear membrane protein Bqt4 recruits phosphatidic acid to the nuclear envelope to maintain its structural integrity.
Hirano Y et al. J Biol Chem 2024 May 31;:107430
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:23695302 - Functional characterization of fission yeast transcription factors by overexpression analysis.
Vachon L et al. Genetics 2013 Aug;194(4):873-84
PMID:18257517 - Phosphoproteome analysis of fission yeast.
Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
PMID:39892963 - The losses of Lem2 and Bqt4 exhibit similar impacts on intracellular movement dynamics in fission yeast.
Wang K et al. Biochem Biophys Res Commun 2025 Feb 16;749:151326
PMID:27984725 - CDK Substrate Phosphorylation and Ordering the Cell Cycle.
Swaffer MP et al. Cell 2016 Dec 15;167(7):1750-1761.e16
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:30975915 - The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function in fission yeast cells.
Kinugasa Y et al. J Cell Sci 2019 May 15;132(10)
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:31064814 - Proximity-dependent biotinylation mediated by TurboID to identify protein-protein interaction networks in yeast.
Larochelle M et al. J Cell Sci 2019 May 31;132(11)
PMID:29292846 - Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast.
Hirano Y et al. Genes Cells 2018 Mar;23(3):122-135
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:22959349 - Telomere-nuclear envelope dissociation promoted by Rap1 phosphorylation ensures faithful chromosome segregation.
Fujita I et al. Curr Biol 2012 Oct 23;22(20):1932-7
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:38663097 - Bqt4 affects relative movement between SPB and nucleolus in fission yeast.
Wang K et al. Biochem Biophys Res Commun 2024 Apr 23;714:149970
PMID:31883795 - Positioning Heterochromatin at the Nuclear Periphery Suppresses Histone Turnover to Promote Epigenetic Inheritance.
Holla S et al. Cell 2020 Jan 09;180(1):150-164.e15
PMID:37078207 - Ceramide synthase homolog Tlc4 maintains nuclear envelope integrity via its Golgi translocation.
Hirano Y et al. J Cell Sci 2023 May 15;136(10)
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:30503780 - The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope.
Hu C et al. Structure 2019 Feb 05;27(2):335-343.e3
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:36799444 - Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast.
Hirano Y et al. J Biochem 2023 Jun 30;174(1):33-46
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.
Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:26404184 - High Confidence Fission Yeast SUMO Conjugates Identified by Tandem Denaturing Affinity Purification.
Nie M et al. Sci Rep 2015 Sep 25;5:14389
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:25798942 - The Fun30 chromatin remodeler Fft3 controls nuclear organization and chromatin structure of insulators and subtelomeres in fission yeast.
Steglich B et al. PLoS Genet 2015 Mar;11(3):e1005101
PMID:27334362 - Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions.
Tange Y et al. Genes Cells 2016 Aug;21(8):812-32
PMID:31980821 - Nuclear envelope attachment of telomeres limits TERRA and telomeric rearrangements in quiescent fission yeast cells.
Maestroni L et al. Nucleic Acids Res 2020 Apr 06;48(6):3029-3041
PMID:37939137 - The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast.
Zou CX et al. PLoS Biol 2023 Nov;21(11):e3002372