protein coding gene - mad2 (SPBC20F10.06) - mitotic spindle checkpoint protein Mad2

Gene summary

Standard name

mad2

Systematic ID

SPBC20F10.06

Product

mitotic spindle checkpoint protein Mad2

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O14417

ORFeome ID

40/40A08

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 3294275..3295195 forward strand

Annotation

Protein features

Orthologs

• MAD2L1 (HGNC:6763) Homo sapiens
• mad2 (SJAG_01330) Schizosaccharomyces japonicus
• MAD2 (YJL030W) Saccharomyces cerevisiae

References / Literature

PMID:32361273 - Requirement of PP2A-B56 Par1 for the Stabilization of the CDK Inhibitor Rum1 and Activation of APC/C Ste9 during Pre-Start G1 in S. pombe.

Stonyte V et al. iScience 2020 May 22;23(5):101063

PMID:16481403 - The fission yeast transforming acidic coiled coil-related protein Mia1p/Alp7p is required for formation and maintenance of persistent microtubule-organizing centers at the nuclear envelope.

Zheng L et al. Mol Biol Cell 2006 May;17(5):2212-22

PMID:21664573 - Spindle checkpoint silencing requires association of PP1 to both Spc7 and kinesin-8 motors.

Meadows JC et al. Dev Cell 2011 Jun 14;20(6):739-50

PMID:22281223 - Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.

Zich J et al. Curr Biol 2012 Feb 21;22(4):296-301

PMID:20375067 - Fission yeast Rad26ATRIP delays spindle-pole-body separation following interphase microtubule damage.

Herring M et al. J Cell Sci 2010 May 01;123(Pt 9):1537-45

PMID:11909965 - Fission yeast Mad3p is required for Mad2p to inhibit the anaphase-promoting complex and localizes to kinetochores in a Bub1p-, Bub3p-, and Mph1p-dependent manner.

Millband DN et al. Mol Cell Biol 2002 Apr;22(8):2728-42

PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.

Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704

PMID:10749926 - A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p.

Paluh JL et al. Mol Biol Cell 2000 Apr;11(4):1225-39

PMID:28974540 - The fission yeast nucleoporin Alm1 is required for proteasomal degradation of kinetochore components.

Salas-Pino S et al. J Cell Biol 2017 Nov 06;216(11):3591-3608

PMID:33529549 - Chiasmata and the kinetochore component Dam1 are crucial for elimination of erroneous chromosome attachments and centromere oscillation at meiosis I.

Wakiya M et al. Open Biol 2021 Feb;11(2):200308

PMID:27350684 - Mutant allele of rna14 in fission yeast affects pre-mRNA splicing.

Yadav S et al. J Genet 2016 Jun;95(2):389-97

PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.

Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684

PMID:26510788 - Escape from Mitotic Arrest: An Unexpected Connection Between Microtubule Dynamics and Epigenetic Regulation of Centromeric Chromatin in Schizosaccharomyces pombe.

George AA et al. Genetics 2015 Dec;201(4):1467-78

PMID:22660415 - MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.

Yamagishi Y et al. Nat Cell Biol 2012 Jun 03;14(7):746-52

PMID:12242294 - The mal2p protein is an essential component of the fission yeast centromere.

Jin QW et al. Mol Cell Biol 2002 Oct;22(20):7168-83

PMID:23394829 - Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways.

Gupta S et al. Curr Biol 2013 Feb 18;23(4):333-8

PMID:21920317 - Repositioning of aurora B promoted by chiasmata ensures sister chromatid mono-orientation in meiosis I.

Sakuno T et al. Dev Cell 2011 Sep 13;21(3):534-45

PMID:26900649 - The hairpin region of Ndc80 is important for the kinetochore recruitment of Mph1/MPS1 in fission yeast.

Chmielewska AE et al. Cell Cycle 2016;15(5):740-7

PMID:23427262 - The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment.

Tang NH et al. Mol Biol Cell 2013 Apr;24(8):1122-33

PMID:20498706 - Tip1/CLIP-170 protein is required for correct chromosome poleward movement in fission yeast.

Goldstone S et al. PLoS One 2010 May 13;5(5):e10634

PMID:28497540 - Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.

Miyazaki S et al. Genes Cells 2017 Jun;22(6):552-567

PMID:26031557 - Loss of kinesin-14 results in aneuploidy via kinesin-5-dependent microtubule protrusions leading to chromosome cut.

Syrovatkina V et al. Nat Commun 2015 Jun 02;6:7322

PMID:31257143 - The Bub1-TPR Domain Interacts Directly with Mad3 to Generate Robust Spindle Checkpoint Arrest.

Leontiou I et al. Curr Biol 2019 Jul 22;29(14):2407-2414.e7

PMID:28017606 - Generation of a Spindle Checkpoint Arrest from Synthetic Signaling Assemblies.

Yuan I et al. Curr Biol 2017 Jan 09;27(1):137-143

PMID:28410370 - A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.

Dudin O et al. PLoS Genet 2017 Apr;13(4):e1006721

PMID:29813053 - Specific detection of fission yeast primary septum reveals septum and cleavage furrow ingression during early anaphase independent of mitosis completion.

G Cortés JC et al. PLoS Genet 2018 May;14(5):e1007388

PMID:25253718 - csi2p modulates microtubule dynamics and organizes the bipolar spindle for chromosome segregation.

Costa J et al. Mol Biol Cell 2014 Dec 01;25(24):3900-8

PMID:19523829 - Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation.

Choi SH et al. Curr Biol 2009 Jun 23;19(12):985-95

PMID:11882285 - Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast.

Toyoda Y et al. Curr Biol 2002 Mar 05;12(5):347-58

PMID:31000521 - Overlapping Roles in Chromosome Segregation for Heterochromatin Protein 1 (Swi6) and DDK in Schizosaccharomyces pombe .

Shen KF et al. Genetics 2019 Jun;212(2):417-430

PMID:36108046 - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.

Bai S et al. PLoS Genet 2022 Sep;18(9):e1010397

PMID:17277378 - Novel mad2 alleles isolated in a Schizosaccharomyces pombe gamma-tubulin mutant are defective in metaphase arrest activity, but remain functional for chromosome stability in unperturbed mitosis.

Tange Y et al. Genetics 2007 Apr;175(4):1571-84

PMID:21965289 - Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.

Buttrick GJ et al. Mol Biol Cell 2011 Dec;22(23):4486-502

PMID:17004072 - Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.

Fedyanina OS et al. Curr Genet 2006 Nov;50(5):281-94

PMID:12676091 - S. pombe aurora kinase/survivin is required for chromosome condensation and the spindle checkpoint attachment response.

Petersen J et al. Curr Biol 2003 Apr 01;13(7):590-7

PMID:29021344 - A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.

Yukawa M et al. Mol Biol Cell 2017 Dec 01;28(25):3647-3659

PMID:18818364 - Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.

Roguev A et al. Science 2008 Oct 17;322(5900):405-10

PMID:15483052 - BAF53/Arp4 homolog Alp5 in fission yeast is required for histone H4 acetylation, kinetochore-spindle attachment, and gene silencing at centromere.

Minoda A et al. Mol Biol Cell 2005 Jan;16(1):316-27

PMID:28366744 - Fission Yeast Apc15 Stabilizes MCC-Cdc20-APC/C Complexes, Ensuring Efficient Cdc20 Ubiquitination and Checkpoint Arrest.

May KM et al. Curr Biol 2017 Apr 24;27(8):1221-1228

PMID:10775265 - The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast.

Guertin DA et al. EMBO J 2000 Apr 17;19(8):1803-15

PMID:22375062 - Plo1 phosphorylates Dam1 to promote chromosome bi-orientation in fission yeast.

Buttrick GJ et al. J Cell Sci 2012 Apr 01;125(Pt 7):1645-51

PMID:26527280 - Robust Ordering of Anaphase Events by Adaptive Thresholds and Competing Degradation Pathways.

Kamenz J et al. Mol Cell 2015 Nov 05;60(3):446-59

PMID:11967147 - Two kinesin-like Kin I family proteins in fission yeast regulate the establishment of metaphase and the onset of anaphase A.

Garcia MA et al. Curr Biol 2002 Apr 16;12(8):610-21

PMID:11058086 - High dosage expression of a zinc finger protein, Grt1, suppresses a mutant of fission yeast slp1(+), a homolog of CDC20/p55CDC/Fizzy.

Yamada HY et al. J Cell Sci 2000 Nov;113 ( Pt 22):3989-99

PMID:22437499 - Structure of the mitotic checkpoint complex.

Chao WC et al. Nature 2012 Mar 21;484(7393):208-13

PMID:15769255 - Nep1, a Schizosaccharomyces pombe deneddylating enzyme.

Zhou L et al. Biochem J 2005 Jul 15;389(Pt 2):307-14

PMID:23442800 - An E2 enzyme Ubc11 is required for ubiquitination of Slp1/Cdc20 and spindle checkpoint silencing in fission yeast.

Horikoshi Y et al. Cell Cycle 2013 Mar 15;12(6):961-71

PMID:40193710 - An enzymatic-independent function of palmitoyl hydrolase in cohesin loading onto chromosome.

Wang YT et al. Nucleic Acids Res 2025 Mar 20;53(6)

PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.

Rodríguez-López M et al. Elife 2023 Oct 03;12

PMID:11950879 - Control of localization of a spindle checkpoint protein, Mad2, in fission yeast.

Ikui AE et al. J Cell Sci 2002 Apr 15;115(Pt 8):1603-10

PMID:24583014 - Slow checkpoint activation kinetics as a safety device in anaphase.

Kamenz J et al. Curr Biol 2014 Mar 17;24(6):646-51

PMID:18931302 - Significant conservation of synthetic lethal genetic interaction networks between distantly related eukaryotes.

Dixon SJ et al. Proc Natl Acad Sci U S A 2008 Oct 28;105(43):16653-8

PMID:9223296 - The Schizosaccharomyces pombe spindle checkpoint protein mad2p blocks anaphase and genetically interacts with the anaphase-promoting complex.

He X et al. Proc Natl Acad Sci U S A 1997 Jul 22;94(15):7965-70

PMID:16394105 - The V260I mutation in fission yeast alpha-tubulin Atb2 affects microtubule dynamics and EB1-Mal3 localization and activates the Bub1 branch of the spindle checkpoint.

Asakawa K et al. Mol Biol Cell 2006 Mar;17(3):1421-35

PMID:16478992 - Requirement of fission yeast Cid14 in polyadenylation of rRNAs.

Win TZ et al. Mol Cell Biol 2006 Mar;26(5):1710-21

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:26581324 - Cleavage and polyadenylation factor, Rna14 is an essential protein required for the maintenance of genomic integrity in fission yeast Schizosaccharomyces pombe.

Sonkar A et al. Biochim Biophys Acta 2016 Feb;1863(2):189-97

PMID:33260998 - High-Throughput Flow Cytometry Combined with Genetic Analysis Brings New Insights into the Understanding of Chromatin Regulation of Cellular Quiescence.

Zahedi Y et al. Int J Mol Sci 2020 Nov 27;21(23)

PMID:10779336 - Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase.

Tanaka K et al. Mol Cell Biol 2000 May;20(10):3459-69

PMID:9742398 - Conjugation in S. pombe: identification of a microtubule-organising centre, a requirement for microtubules and a role for Mad2.

Petersen J et al. Curr Biol 1998 Aug 27;8(17):963-6

PMID:9601094 - Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe.

He X et al. J Cell Sci 1998 Jun;111 ( Pt 12):1635-47

PMID:23166349 - Csi1 links centromeres to the nuclear envelope for centromere clustering.

Hou H et al. J Cell Biol 2012 Nov 26;199(5):735-44

PMID:19723888 - Inhibition of type I histone deacetylase increases resistance of checkpoint-deficient cells to genotoxic agents through mitotic delay.

Alao JP et al. Mol Cancer Ther 2009 Sep;8(9):2606-15

PMID:21423721 - Chiasmata promote monopolar attachment of sister chromatids and their co-segregation toward the proper pole during meiosis I.

Hirose Y et al. PLoS Genet 2011 Mar;7(3):e1001329

PMID:25472718 - Alp7/TACC recruits kinesin-8-PP1 to the Ndc80 kinetochore protein for timely mitotic progression and chromosome movement.

Tang NH et al. J Cell Sci 2015 Jan 15;128(2):354-63

PMID:26882497 - Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.

Zich J et al. PLoS Genet 2016 Feb;12(2):e1005834

PMID:26696398 - Autophagy is required for efficient meiosis progression and proper meiotic chromosome segregation in fission yeast.

Matsuhara H et al. Genes Cells 2016 Jan;21(1):65-87

PMID:33506191 - Escape from mitotic catastrophe by actin-dependent nuclear displacement in fission yeast.

Yukawa M et al. iScience 2021 Jan 22;24(1):102031

PMID:38166399 - Fission yeast Wee1 is required for stable kinetochore-microtubule attachment.

Takado M et al. Open Biol 2024 Jan;14(1):230379

PMID:12606573 - Function of Cdc2p-dependent Bub1p phosphorylation and Bub1p kinase activity in the mitotic and meiotic spindle checkpoint.

Yamaguchi S et al. EMBO J 2003 Mar 03;22(5):1075-87

PMID:10459013 - Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis.

Sparks CA et al. J Cell Biol 1999 Aug 23;146(4):777-90

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:22425159 - Transient structure associated with the spindle pole body directs meiotic microtubule reorganization in S. pombe.

Funaya C et al. Curr Biol 2012 Apr 10;22(7):562-74

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:19592249 - A novel protein phosphatase 1-dependent spindle checkpoint silencing mechanism.

Vanoosthuyse V et al. Curr Biol 2009 Jul 28;19(14):1176-81

PMID:12951601 - Deletion of Mia1/Alp7 activates Mad2-dependent spindle assembly checkpoint in fission yeast.

Sato M et al. Nat Cell Biol 2003 Sep;5(9):764-6; author reply 766

PMID:16855399 - An extended anaphase signaling pathway for Mad2p includes microtubule organizing center proteins and multiple motor-dependent transitions.

Mayer C et al. Cell Cycle 2006 Jul;5(13):1456-63

PMID:12426374 - Spindle-kinetochore attachment requires the combined action of Kin I-like Klp5/6 and Alp14/Dis1-MAPs in fission yeast.

Garcia MA et al. EMBO J 2002 Nov 15;21(22):6015-24

PMID:24161933 - Determinants of robustness in spindle assembly checkpoint signalling.

Heinrich S et al. Nat Cell Biol 2013 Nov;15(11):1328-39

PMID:25375240 - The kinetochore protein Kis1/Eic1/Mis19 ensures the integrity of mitotic spindles through maintenance of kinetochore factors Mis6/CENP-I and CENP-A.

Hirai H et al. PLoS One 2014;9(11):e111905

PMID:15347659 - DNA replication checkpoint control mediated by the spindle checkpoint protein Mad2p in fission yeast.

Sugimoto I et al. J Biol Chem 2004 Nov 05;279(45):47372-8

PMID:25764183 - Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast.

Xu X et al. PLoS One 2015;10(3):e0119347

PMID:28366743 - Different Functionality of Cdc20 Binding Sites within the Mitotic Checkpoint Complex.

Sewart K et al. Curr Biol 2017 Apr 24;27(8):1213-1220

PMID:27618268 - Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.

Mora-Santos MD et al. Curr Biol 2016 Oct 10;26(19):2642-2650

PMID:35811551 - Mitotic checkpoint gene expression is tuned by codon usage bias.

Esposito E et al. EMBO J 2022 Aug 01;41(15):e107896

PMID:22825872 - Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.

Heinrich S et al. J Cell Sci 2012 Oct 15;125(Pt 20):4720-7

PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.

Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54

PMID:37288768 - Altered cohesin dynamics and H3K9 modifications contribute to mitotic defects in the cbf11Δ lipid metabolism mutant.

Vishwanatha A et al. J Cell Sci 2023 Jun 01;136(11)

PMID:16824200 - Schizosaccharomyces pombe homolog of Survivin, Bir1p, exhibits a novel dynamic behavior at the spindle mid-zone.

Rajagopalan S et al. Genes Cells 2006 Jul;11(7):815-27

PMID:26258632 - Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.

Akera T et al. Nat Cell Biol 2015 Sep;17(9):1124-33

PMID:26483559 - Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly.

Yang HJ et al. J Cell Biol 2015 Oct 26;211(2):295-308

PMID:28178520 - Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.

Meadows JC et al. Cell Rep 2017 Feb 07;18(6):1422-1433

PMID:28513584 - Kinesin-5-independent mitotic spindle assembly requires the antiparallel microtubule crosslinker Ase1 in fission yeast.

Rincon SA et al. Nat Commun 2017 May 17;8:15286

PMID:16079915 - The DASH complex and Klp5/Klp6 kinesin coordinate bipolar chromosome attachment in fission yeast.

Sanchez-Perez I et al. EMBO J 2005 Aug 17;24(16):2931-43

PMID:18414064 - Schizosaccharomyces pombe Orc5 plays multiple roles in the maintenance of genome stability throughout the cell cycle.

Kato H et al. Cell Cycle 2008 Apr 15;7(8):1085-96

PMID:17380189 - Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function.

Murakami H et al. PLoS One 2007 Mar 21;2(3):e317

PMID:18562692 - Latrunculin A delays anaphase onset in fission yeast by disrupting an Ase1-independent pathway controlling mitotic spindle stability.

Meadows JC et al. Mol Biol Cell 2008 Sep;19(9):3713-23

PMID:27664031 - C-terminal region of Mad2 plays an important role during mitotic spindle checkpoint in fission yeast Schizosaccharomyces pombe.

Singh GK et al. Mol Biol Rep 2017 Feb;44(1):89-96

PMID:20967237 - Fission yeast cells undergo nuclear division in the absence of spindle microtubules.

Castagnetti S et al. PLoS Biol 2010 Oct 12;8(10):e1000512

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:28567704 - Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity.

Sonkar A et al. Mol Genet Genomics 2017 Oct;292(5):1027-1036

PMID:19736319 - Proper timing of cytokinesis is regulated by Schizosaccharomyces pombe Etd1.

García-Cortés JC et al. J Cell Biol 2009 Sep 07;186(5):739-53

PMID:16303848 - A role for the fission yeast Rqh1 helicase in chromosome segregation.

Win TZ et al. J Cell Sci 2005 Dec 15;118(Pt 24):5777-84

PMID:11460168 - A MAP kinase-dependent actin checkpoint ensures proper spindle orientation in fission yeast.

Gachet Y et al. Nature 2001 Jul 19;412(6844):352-5

PMID:18082611 - Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1.

Ohi MD et al. Mol Cell 2007 Dec 14;28(5):871-85

PMID:16360688 - Control of Shugoshin function during fission-yeast meiosis.

Vaur S et al. Curr Biol 2005 Dec 20;15(24):2263-70

PMID:27798241 - An acetyltransferase-independent function of Eso1 regulates centromere cohesion.

Lin SJ et al. Mol Biol Cell 2016 Dec 15;27(25):4002-4010

PMID:19168987 - Essential roles of Snf21, a Swi2/Snf2 family chromatin remodeler, in fission yeast mitosis.

Yamada K et al. Genes Genet Syst 2008 Oct;83(5):361-72

PMID:11331883 - Fission yeast Bub1 is essential in setting up the meiotic pattern of chromosome segregation.

Bernard P et al. Nat Cell Biol 2001 May;3(5):522-6

PMID:12065422 - Schizosaccharomyces pombe NIMA-related kinase, Fin1, regulates spindle formation and an affinity of Polo for the SPB.

Grallert A et al. EMBO J 2002 Jun 17;21(12):3096-107

PMID:12719471 - Sim4: a novel fission yeast kinetochore protein required for centromeric silencing and chromosome segregation.

Pidoux AL et al. J Cell Biol 2003 Apr 28;161(2):295-307

PMID:18354085 - Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.

Kimata Y et al. J Cell Sci 2008 Apr 01;121(Pt 7):1107-18

PMID:22521786 - Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.

Shepperd LA et al. Curr Biol 2012 May 22;22(10):891-9

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.

Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78

PMID:19680287 - Bub1 and Bub3 promote the conversion from monopolar to bipolar chromosome attachment independently of shugoshin.

Windecker H et al. EMBO Rep 2009 Sep;10(9):1022-8

PMID:27697865 - Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe.

Topolski B et al. Mol Cell Biol 2016 Dec 15;36(24):3128-3140

PMID:24477934 - Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.

Heinrich S et al. EMBO Rep 2014 Mar;15(3):291-8

PMID:25076038 - Large scale screening of genetic interaction with sgf73(+) in fission yeast.

Guo Y et al. Yi Chuan 2014 Jul;36(7):723-31

PMID:27053664 - Synergistic role of fission yeast Alp16GCP6 and Mzt1MOZART1 in γ-tubulin complex recruitment to mitotic spindle pole bodies and spindle assembly.

Masuda H et al. Mol Biol Cell 2016 Jun 01;27(11):1753-63

PMID:24239120 - Antagonistic spindle motors and MAPs regulate metaphase spindle length and chromosome segregation.

Syrovatkina V et al. Curr Biol 2013 Dec 02;23(23):2423-9

PMID:18556659 - The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).

Sczaniecka M et al. J Biol Chem 2008 Aug 22;283(34):23039-47

PMID:31615768 - CLASP promotes microtubule bundling in metaphase spindle independently of Ase1/PRC1 in fission yeast.

Ebina H et al. Biol Open 2019 Oct 24;8(10)

PMID:20444100 - Hyperosmosis enhances radiation and hydroxyurea resistance of Schizosaccharomyces pombe checkpoint mutants through the spindle checkpoint and delayed cytokinesis.

Alao JP et al. Mol Microbiol 2010 Jul 01;77(1):143-57

PMID:36537249 - The fission yeast kinetochore complex Mhf1-Mhf2 regulates the spindle assembly checkpoint and faithful chromosome segregation.

Jian Y et al. J Cell Sci 2023 Jan 15;136(2)

PMID:20028739 - Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast.

Bernard P et al. Mol Cell Biol 2010 Mar;30(5):1145-57

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:12186944 - Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase Plo1 to the spindle pole body and a functional spindle assembly checkpoint.

Mulvihill DP et al. J Cell Sci 2002 Sep 15;115(Pt 18):3575-86

PMID:9461438 - Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.

Kim SH et al. Science 1998 Feb 13;279(5353):1045-7

PMID:11432827 - Fission yeast ch-TOG/XMAP215 homologue Alp14 connects mitotic spindles with the kinetochore and is a component of the Mad2-dependent spindle checkpoint.

Garcia MA et al. EMBO J 2001 Jul 02;20(13):3389-401

PMID:28455357 - Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase.

Lin L et al. Biol Open 2017 Jun 15;6(6):844-849

PMID:24937146 - Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast.

Tang NH et al. FEBS Lett 2014 Aug 25;588(17):2814-21

PMID:22184248 - Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.

Ito D et al. Proc Natl Acad Sci U S A 2012 Jan 03;109(1):209-14

PMID:11080156 - The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint.

Vardy L et al. EMBO J 2000 Nov 15;19(22):6098-111

PMID:24469396 - Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.

Nakano A et al. Mol Cell Biol 2014 Apr;34(8):1389-97

PMID:22737087 - The CCR4-NOT complex is implicated in the viability of aneuploid yeasts.

Tange Y et al. PLoS Genet 2012;8(6):e1002776

PMID:12615979 - Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase.

Reynolds N et al. J Cell Sci 2003 Apr 01;116(Pt 7):1377-87

PMID:15930132 - Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.

Saitoh S et al. Mol Biol Cell 2005 Aug;16(8):3666-77