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protein coding gene - mis17 (SPBC21.01) - CENP-U ortholog Mis17

Gene summary

Standard name
mis17
Systematic ID
SPBC21.01
Product
CENP-U ortholog Mis17
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
SPBC776.19
UniProt ID
O94643
ORFeome ID
50/50F10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3214206..3215938 forward strand

Annotation

GO biological process

GO:0034080 - CENP-A containing chromatin assembly

References:

GO cellular component

GO:0000776 - kinetochore

References:

GO:0031511 - Mis6-Sim4 complex

References:

GO:0005634 - nucleus

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00696 - phosphorylated residue

References:

MOD:01149 - sumoylated lysine

References:

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0006238 - decreased protein localization to centromere

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

References:

Genotypes:

FYPO:0000836 - increased protein level

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0001357 - normal vegetative cell population growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0000141 - abnormal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0002256 - abnormal nuclear morphology

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Genotypes:

FYPO:0001269 - abolished protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0003740 - decreased CENP-A containing chromatin assembly

References:

Genotypes:

FYPO:0000835 - decreased protein level

References:

Genotypes:

FYPO:0006238 - decreased protein localization to centromere

References:

Genotypes:

FYPO:0002902 - decreased protein localization to kinetochore during vegetative growth

References:

Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001511 - inviable vegetative cell, abnormal cell shape, normal cell size

References:

Genotypes:

FYPO:0000284 - large and small daughter nuclei, with unequal mitotic sister chromatid segregation

References:

Genotypes:

FYPO:0003241 - unequal mitotic sister chromatid segregation

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF20994CENPUAME1PFAM
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Low-complexitydisorder_predictionMOBIDB-Low-complexity
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:22561346 - CENP-T proteins are conserved centromere receptors of the Ndc80 complex.
Schleiffer A et al. Nat Cell Biol 2012 May 06;14(6):604-13
PMID:17352737 - The fission yeast DASH complex is essential for satisfying the spindle assembly checkpoint induced by defects in the inner-kinetochore proteins.
Kobayashi Y et al. Genes Cells 2007 Mar;12(3):311-28
PMID:16855021 - Fta2, an essential fission yeast kinetochore component, interacts closely with the conserved Mal2 protein.
Kerres A et al. Mol Biol Cell 2006 Oct;17(10):4167-78
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:16079914 - Molecular analysis of kinetochore architecture in fission yeast.
Liu X et al. EMBO J 2005 Aug 17;24(16):2919-30
PMID:19217403 - Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.
Williams JS et al. Mol Cell 2009 Feb 13;33(3):287-98
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:28228545 - Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.
Upadhyay U et al. Mol Biol Cell 2017 Apr 15;28(8):1132-1146
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:30759079 - A systematic genetic screen identifies essential factors involved in nuclear size control.
Cantwell H et al. PLoS Genet 2019 Feb;15(2):e1007929
PMID:28827290 - Kinetochore Components Required for Centromeric Chromatin Assembly Are Impacted by Msc1 in Schizosaccharomyces pombe .
Gao C et al. Genetics 2017 Oct;207(2):559-569
PMID:30967422 - Negative Regulation of the Mis17-Mis6 Centromere Complex by mRNA Decay Pathway and EKC/KEOPS Complex in Schizosaccharomyces pombe .
Xu X et al. G3 (Bethesda) 2019 Jun 05;9(6):1815-1823
PMID:36200823 - The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary.
Tsunemine S et al. Nucleic Acids Res 2022 Oct 28;50(19):10914-10928
PMID:21445296 - Mis17 is a regulatory module of the Mis6-Mal2-Sim4 centromere complex that is required for the recruitment of CenH3/CENP-A in fission yeast.
Shiroiwa Y et al. PLoS One 2011 Mar 21;6(3):e17761
PMID:15369671 - Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres.
Hayashi T et al. Cell 2004 Sep 17;118(6):715-29
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:17442892 - The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.
Kerres A et al. Mol Biol Cell 2007 Jul;18(7):2441-54
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623