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protein coding gene - med8 (SPBC21.04) - mediator complex subunit Med8

Gene summary

Standard name
med8
Systematic ID
SPBC21.04
Product
mediator complex subunit Med8
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
sep15
UniProt ID
O94646
ORFeome ID
43/43A10
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3219405..3222152 forward strand

Annotation

Comment

PBO:0000206 - deletion mutant expression profiling

References:

GO biological process

GO:0060261 - positive regulation of transcription initiation by RNA polymerase II

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GO:0006367 - transcription initiation at RNA polymerase II promoter

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GO cellular component

GO:0070847 - core mediator complex

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GO:0016592 - mediator complex

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GO:0005634 - nucleus

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GO molecular function

GO:0005515 - protein binding

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GO:0000978 - RNA polymerase II cis-regulatory region sequence-specific DNA binding

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GO:0003713 - transcription coactivator activity

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Multi-locus phenotype

FYPO:0004562 - binucleate aseptate vegetative cell

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Genotypes:

FYPO:0002150 - inviable spore population

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Genotypes:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000117 - abnormal septum assembly

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Genotypes:

FYPO:0002023 - abnormal septum morphology during vegetative growth

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Genotypes:

FYPO:0005853 - aggregated filamentous actin in cytoplasm

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Genotypes:

FYPO:0000134 - branched, elongated, multiseptate cell

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Genotypes:

FYPO:0000581 - decreased spore germination frequency

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Genotypes:

FYPO:0000584 - decreased sporulation frequency

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Genotypes:

FYPO:0000223 - elongated multiseptate vegetative cell

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Genotypes:

FYPO:0002282 - inviable curved septated vegetative cell

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0003717 - normal actin cytoskeleton morphology during vegetative growth

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Genotypes:

FYPO:0000962 - normal growth on hydrogen peroxide

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Genotypes:

FYPO:0000961 - normal growth on sorbitol

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Genotypes:

FYPO:0006213 - normal septum morphology

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Genotypes:

FYPO:0004263 - normal viability in stationary phase during nitrogen limitation

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Genotypes:

FYPO:0002047 - resistance to heat shock during nitrogen starvation

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Genotypes:

FYPO:0000097 - sensitive to caffeine during vegetative growth

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Genotypes:

FYPO:0001408 - sensitive to heat shock

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Genotypes:

FYPO:0006836 - sensitive to magnesium chloride

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0005889 - sensitive to sodium chloride

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Genotypes:

FYPO:0000116 - sensitive to zinc

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

FYPO:0000280 - sterile

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF10232Med8Mediatior_Med8_fun/metPFAM
G3DSA:1.20.58.1710:FF:000002FUNFAM
G3DSA:1.20.5.790:FF:000002FUNFAM
G3DSA:6.10.250.2610GENE3D
G3DSA:1.20.5.790Single helix binGENE3D
G3DSA:1.20.58.1710GENE3D
PTHR13074MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8Mediatior_Med8_fun/metPANTHER

Orthologs

References / Literature

PMID:10392445 - Eleven novel sep genes of Schizosaccharomyces pombe required for efficient cell separation and sexual differentiation.
Grallert A et al. Yeast 1999 Jun 15;15(8):669-86
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28467824 - Core Mediator structure at 3.4 Å extends model of transcription initiation complex.
Nozawa K et al. Nature 2017 May 11;545(7653):248-251
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
Pfam:PF10232 - Unknown title
PMID:23123849 - Structure of the Mediator head module.
Larivière L et al. Nature 2012 Dec 20;492(7429):448-51
PMID:18381891 - Structure-system correlation identifies a gene regulatory Mediator submodule.
Larivière L et al. Genes Dev 2008 Apr 01;22(7):872-7
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:12738880 - TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally conserved through evolution.
Samuelsen CO et al. Proc Natl Acad Sci U S A 2003 May 27;100(11):6422-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
PMID:18310102 - Two conserved modules of Schizosaccharomyces pombe Mediator regulate distinct cellular pathways.
Linder T et al. Nucleic Acids Res 2008 May;36(8):2489-504
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:11572939 - Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells.
Spåhr H et al. Proc Natl Acad Sci U S A 2001 Oct 09;98(21):11985-90
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:17922236 - Genomic expression patterns in cell separation mutants of Schizosaccharomyces pombe defective in the genes sep10 ( + ) and sep15 ( + ) coding for the Mediator subunits Med31 and Med8.
Miklos I et al. Mol Genet Genomics 2008 Mar;279(3):225-38
PMID:11191205 - The Schizosaccharomyces pombe sep15+ gene encodes a protein homologous to the Med8 subunit of the Saccharomyces cerevisiae transcriptional mediator complex.
Zilahi E et al. Curr Genet 2000 Dec;38(5):227-32
PMID:19473263 - The involvement of the Schizosaccharomyces pombe sep9/spt8 gene in the regulation of septum cleavage.
Batta G et al. FEMS Yeast Res 2009 Aug;9(5):757-67
PMID:28241144 - Mediator structure and rearrangements required for holoenzyme formation.
Tsai KL et al. Nature 2017 Apr 13;544(7649):196-201
PMID:19720063 - The Med8 mediator subunit interacts with the Rpb4 subunit of RNA polymerase II and Ace2 transcriptional activator in Schizosaccharomyces pombe.
Mehta S et al. FEBS Lett 2009 Oct 06;583(19):3115-20
PMID:15829570 - Impairment of the TFIIH-associated CDK-activating kinase selectively affects cell cycle-regulated gene expression in fission yeast.
Lee KM et al. Mol Biol Cell 2005 Jun;16(6):2734-45
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:15182371 - Mediator is required for activated transcription in a Schizosaccharomyces pombe in vitro system.
Tamayo E et al. Eur J Biochem 2004 Jun;271(12):2561-72
PMID:10625684 - Purification and characterization of RNA polymerase II holoenzyme from Schizosaccharomyces pombe.
Spåhr H et al. J Biol Chem 2000 Jan 14;275(2):1351-6