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protein coding gene - cwf3 (SPBC211.02c) - Prp19 complex subunit Cwf3

Gene summary

Standard name
cwf3
Systematic ID
SPBC211.02c
Product
Prp19 complex subunit Cwf3
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
syf1
UniProt ID
Q9P7R9
ORFeome ID
46/46H05
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3870710..3873685 reverse strand

Annotation

GO biological process

GO:0000349 - generation of catalytic spliceosome for first transesterification step

References:

GO:0045292 - mRNA cis splicing, via spliceosome

References:

GO cellular component

GO:0005829 - cytosol

References:

GO:0005634 - nucleus

References:

GO:0071014 - post-mRNA release spliceosomal complex

References:

GO:0000974 - Prp19 complex

References:

GO:0005681 - spliceosomal complex

References:

GO:0071007 - U2-type catalytic step 2 spliceosome

References:

GO:0071004 - U2-type prespliceosome

References:

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF23220HAT_Syf1_MHAT_Syf1_centralPFAM
PF23233HAT_Syf1_CNRKL1_NHAT_Syf1-like_NPFAM
PF23231HAT_Syf1_CNRKL1_CHAT_Syf1_CNRKL1_CPFAM
SM00386hat_new_1HATSMART
G3DSA:1.25.40.10:FF:000023FUNFAM
G3DSA:1.25.40.10:FF:000666FUNFAM
SSF48452TPR-likeTPR-like_helical_dom_sfSUPERFAMILY
G3DSA:1.25.40.10Tetratricopeptide repeat domainTPR-like_helical_dom_sfGENE3D
PTHR11246PRE-MRNA SPLICING FACTORSyf1-likePANTHER
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polardisorder_predictionMOBIDB-Polar

Orthologs

References / Literature

PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
Budden AM et al. Elife 2023 Aug 24;12
PMID:39833470 - Structures of aberrant spliceosome intermediates on their way to disassembly.
Soni K et al. Nat Struct Mol Biol 2025 Jan 20;
PMID:23695164 - Cross-species protein interactome mapping reveals species-specific wiring of stress response pathways.
Das J et al. Sci Signal 2013 May 21;6(276):ra38
PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.
Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:10409726 - Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.
McDonald WH et al. Mol Cell Biol 1999 Aug;19(8):5352-62
PMID:12088152 - Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.
Ohi MD et al. RNA 2002 Jun;8(6):798-815
PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.
Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31
PMID:26292707 - Structure of a yeast spliceosome at 3.6-angstrom resolution.
Yan C et al. Science 2015 Sep 11;349(6253):1182-91
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.
Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PMID:11751918 - The fission yeast ES2 homologue, Bis1, interacts with the Ish1 stress-responsive nuclear envelope protein.
Taricani L et al. J Biol Chem 2002 Mar 22;277(12):10562-72
PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.
Chen W et al. RNA 2014 Mar;20(3):308-20
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:11884590 - Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.
Ohi MD et al. Mol Cell Biol 2002 Apr;22(7):2011-24
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:24874881 - The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.
Kallgren SP et al. PLoS Genet 2014;10(5):e1004334
PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .
Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536
PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.
Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:26682798 - The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.
Aronica L et al. Nucleic Acids Res 2016 Feb 29;44(4):1703-17
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.
Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105