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protein coding gene - arg4 (SPBC215.08c) - arginine specific carbamoyl-phosphate synthase Arg4

Gene summary

Standard name
arg4
Systematic ID
SPBC215.08c
Product
arginine specific carbamoyl-phosphate synthase Arg4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O94313
ORFeome ID
30/30E04
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4041439..4045331 reverse strand

Annotation

PBO:0000567 - 6.3.5.5

Disease association

MONDO:0009376 - carbamoyl phosphate synthetase I deficiency disease

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GO biological process

GO:0006526 - L-arginine biosynthetic process

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GO cellular component

GO:0005951 - carbamoyl-phosphate synthase complex

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GO:0005759 - mitochondrial matrix

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GO:0005739 - mitochondrion

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GO molecular function

GO:0005524 - ATP binding

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GO:0004087 - carbamoyl-phosphate synthase (ammonia) activity

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GO:0004088 - carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

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GO:0046872 - metal ion binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:01148 - ubiquitinylated lysine

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Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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PomGeneEx:0000011 - RNA level increased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0009100 - decreased cell population growth on glycerol and galactose carbon source

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Genotypes:

FYPO:0009091 - decreased cell population growth on lysine and proline nitrogen source

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Genotypes:

FYPO:0009092 - decreased cell population growth on lysine and serine nitrogen source

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Genotypes:

FYPO:0009099 - decreased cell population growth on mannitol carbon source

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Genotypes:

FYPO:0000250 - decreased cell population growth on proline nitrogen source

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Genotypes:

FYPO:0007562 - decreased cell population growth on serine nitrogen source

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Genotypes:

FYPO:0000035 - growth auxotrophic for arginine

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Genotypes:

FYPO:0009052 - increased cell population growth on glutamate nitrogen source

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Genotypes:

FYPO:0009072 - increased cell population growth on lysine nitrogen source

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Genotypes:

FYPO:0000245 - loss of viability in stationary phase

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Genotypes:

FYPO:0009041 - resistance to 2,2′-dipyridyl

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Genotypes:

FYPO:0009030 - resistance to amitrole

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Genotypes:

FYPO:0009068 - resistance to ciclopirox olamine

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Genotypes:

FYPO:0009038 - resistance to egtazic acid

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Genotypes:

FYPO:0002767 - resistance to terbinafine

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Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

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Genotypes:

FYPO:0002988 - sensitive to ammonium

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Genotypes:

FYPO:0009067 - sensitive to amorolfine

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Genotypes:

FYPO:0006680 - sensitive to bisphenol A

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Genotypes:

FYPO:0000095 - sensitive to bleomycin

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Genotypes:

FYPO:0009080 - sensitive to calcofluor and sodium dodecyl sulfate

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Genotypes:

FYPO:0000842 - sensitive to ethanol during vegetative growth

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Genotypes:

FYPO:0000785 - sensitive to formamide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0001214 - sensitive to potassium chloride

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Genotypes:

FYPO:0007924 - sensitive to potassium chloride and sodium dodecyl sulfate

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Genotypes:

FYPO:0007938 - sensitive to tea tree oil

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Genotypes:

FYPO:0002701 - sensitive to torin1

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Genotypes:

FYPO:0000115 - sensitive to valproic acid

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Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

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Genotypes:

Taxonomic conservation

PBO:0011067 - conserved in bacteria

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF02786CPSase_L_D2CPAse_ATP-bdPfam
PF02787CPSase_L_D3CPSase_lsu_oligoPfam
PF25596CPSase_L_D1CPSase_preATP-graspPfam
cd01423MGS_CPS_I_IIICDD
PS00866CPSASE_1CPAse_ATP-bdPROSITE patterns
PS00867CPSASE_2CPAse_ATP-bdPROSITE patterns
PS50975ATP_GRASPATP-graspPROSITE profiles
PS51855MGSMGS-like_domPROSITE profiles
SM01096CPSase_L_D3CPSase_lsu_oligoSMART
PR00098CPSASECPSase_domPRINTS
G3DSA:1.10.1030.10:FF:000001CATH-FunFam
G3DSA:3.30.1490.20:FF:000001CATH-FunFam
G3DSA:3.30.470.20:FF:000001CATH-FunFam
G3DSA:3.30.470.20:FF:000004CATH-FunFam
G3DSA:3.40.50.1380:FF:000015CATH-FunFam
G3DSA:3.40.50.20:FF:000001CATH-FunFam
G3DSA:3.40.50.20:FF:000002CATH-FunFam
G3DSA:1.10.1030.10CarbamoylP_synth_lsu_oligo_sfCATH-Gene3D
G3DSA:3.30.1490.20ATP_grasp_subdomain_1CATH-Gene3D
G3DSA:3.30.470.20CATH-Gene3D
G3DSA:3.40.50.1380MGS-like_dom_sfCATH-Gene3D
G3DSA:3.40.50.20CATH-Gene3D
SSF48108CarbamoylP_synth_lsu_oligo_sfSUPERFAMILY
SSF52335MGS-like_dom_sfSUPERFAMILY
SSF52440PreATP-grasp_dom_sfSUPERFAMILY
SSF56059SUPERFAMILY
PTHR11405PANTHER
NF003671carBNCBIFAM
NF009455PRK12815.1NCBIFAM
TIGR01369CPSaseII_lrgCPSase_lsuNCBIFAM

Orthologs

References / Literature

PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
PMID:22992726 - The SAGA histone acetyltransferase complex regulates leucine uptake through the Agp3 permease in fission yeast.
Takahashi H et al. J Biol Chem 2012 Nov 02;287(45):38158-67
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.
Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
PMID:34250083 - Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
Romila CA et al. Microb Cell 2021 Jul 05;8(7):146-160
GO_REF:0000111 - Gene Ontology annotations Inferred by Curator (IC) using at least one Inferred by Sequence Similarity (ISS) annotation to support the inference
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:36478272 - Translation-complex profiling of fission yeast cells reveals dynamic rearrangements of scanning ribosomal subunits upon nutritional stress.
Duncan CDS et al. Nucleic Acids Res 2022 Dec 09;50(22):13011-13025
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:29432178 - General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
Duncan CDS et al. Proc Natl Acad Sci U S A 2018 Feb 20;115(8):E1829-E1838
PMID:32896087 - Mitochondrial respiration is required to provide amino acids during fermentative proliferation of fission yeast.
Malecki M et al. EMBO Rep 2020 Nov 05;21(11):e50845
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:28281664 - Genetic interactions and functional analyses of the fission yeast gsk3 and amk2 single and double mutants defective in TORC1-dependent processes.
Rallis C et al. Sci Rep 2017 Mar 10;7:44257
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:9211790 - Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.
Benko Z et al. Curr Genet 1997 Jun;31(6):481-7
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:17248775 - Genetic Mapping in SCHIZOSACCHAROMYCES POMBE by Mitotic and Meiotic Analysis and Induced Haploidization.
Kohli J et al. Genetics 1977 Nov;87(3):471-89
PMID:30647105 - Comparative Genomic Screen in Two Yeasts Reveals Conserved Pathways in the Response Network to Phenol Stress.
Alhoch B et al. G3 (Bethesda) 2019 Mar 07;9(3):639-650
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
GO_REF:0000002 - Comments
PMID:26152728 - A Two-step Protein Quality Control Pathway for a Misfolded DJ-1 Variant in Fission Yeast.
Mathiassen SG et al. J Biol Chem 2015 Aug 21;290(34):21141-21153
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:200419 - Change in location of ornithine carbamoyltransferase and carbamoylphosphate synthetase among yeasts in relation to the arginase/ornithine carbamoyltransferase regulatory complex and the energy status of the cells.
Urrestarazu LA et al. Eur J Biochem 1977 Oct 03;79(2):473-81
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)