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protein coding gene - cwf10 (SPBC215.12) - U5 snRNP GTPase subunit Cwf10

Gene summary

Standard name
cwf10
Systematic ID
SPBC215.12
Product
U5 snRNP GTPase subunit Cwf10
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
spef2, snu114, csp4
UniProt ID
O94316
ORFeome ID
30/30A03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 4052556..4055979 forward strand

Annotation

Disease association

MONDO:0012516 - mandibulofacial dysostosis-microcephaly syndrome

References:

GO biological process

GO:0045292 - mRNA cis splicing, via spliceosome

References:

GO:0031047 - regulatory ncRNA-mediated gene silencing

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GO:0140727 - siRNA-mediated pericentric heterochromatin formation

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GO:0000244 - spliceosomal tri-snRNP complex assembly

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GO cellular component

GO:0005829 - cytosol

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GO:0005634 - nucleus

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GO:0005721 - pericentric heterochromatin

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GO:0071014 - post-mRNA release spliceosomal complex

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GO:0000974 - Prp19 complex

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GO:0005681 - spliceosomal complex

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GO:0071007 - U2-type catalytic step 2 spliceosome

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GO:0046540 - U4/U6 x U5 tri-snRNP complex

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GO:0005682 - U5 snRNP

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GO molecular function

GO:0005525 - GTP binding

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GO:0003924 - GTPase activity

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GO:0030623 - U5 snRNA binding

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Modification

MOD:00046 - O-phospho-L-serine

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MOD:00047 - O-phospho-L-threonine

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MOD:00048 - O4'-phospho-L-tyrosine

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MOD:01149 - sumoylated lysine

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Multi-locus phenotype

FYPO:0003231 - decreased histone H3-K9 methylation at heterochromatin domain during vegetative growth

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Genotypes:

FYPO:0003103 - decreased mRNA-derived small RNA level

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Genotypes:

FYPO:0008424 - decreased silent mating-type cassette transcript-derived siRNA level

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Genotypes:

FYPO:0003236 - decreased transposable element-derived small RNA level

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Genotypes:

FYPO:0002052 - normal sporulation frequency

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Genotypes:

FYPO:0006076 - siRNA absent from cell

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Genotypes:

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

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Quantitative gene expression

PBO:0006310 - protein level

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PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0000059 - abnormal mitotic cell cycle

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Genotypes:

FYPO:0010097 - decreased 7-methylguanosine cap hypermethylation

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Genotypes:

FYPO:0003412 - decreased chromatin silencing at centromere outer repeat

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Genotypes:

FYPO:0003096 - decreased histone H3-K9 methylation at centromere outer repeat during vegetative growth

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Genotypes:

FYPO:0003231 - decreased histone H3-K9 methylation at heterochromatin domain during vegetative growth

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Genotypes:

FYPO:0003029 - decreased mRNA splicing, via spliceosome

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Genotypes:

FYPO:0000835 - decreased protein level

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Genotypes:

FYPO:0006599 - decreased protein localization to centromeric chromatin during vegetative growth

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Genotypes:

FYPO:0001645 - decreased protein-protein interaction

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Genotypes:

FYPO:0002134 - decreased protein-RNA interaction

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Genotypes:

FYPO:0008424 - decreased silent mating-type cassette transcript-derived siRNA level

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Genotypes:

FYPO:0004205 - decreased siRNA level

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Genotypes:

FYPO:0000220 - increased centromeric outer repeat transcript level

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Genotypes:

FYPO:0006548 - increased gene expression

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Genotypes:

FYPO:0000836 - increased protein level

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Genotypes:

FYPO:0000825 - increased RNA level during vegetative growth

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Genotypes:

FYPO:0002724 - inviable after spore germination, single or double cell division, elongated cell

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Genotypes:

FYPO:0002379 - inviable after spore germination, without cell division, with elongated germ tube

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Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0003619 - normal mRNA splicing, via spliceosome

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Genotypes:

FYPO:0004083 - normal protein level

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Genotypes:

FYPO:0001408 - sensitive to heat shock

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Genotypes:

FYPO:0001234 - slow vegetative cell population growth

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Warnings

PBO:0000070 - gene structure updated

References:

Protein features

IDNameInterPro nameDB name
PF00009GTP_EFTUT_Tr_GTP-bd_domPfam
PF00679EFG_CEFG_V-likePfam
PF03144GTP_EFTU_D2EFTu-like_2Pfam
PF03764EFG_IVTransl_elong_EFG/EF2_IVPfam
PF16004EFTUD2EFTUD2_NPfam
cd01683EF2_IV_snRNPCDD
cd04090EF2_II_snRNPCDD
cd04098eEF2_C_snRNPU5-116kDa_CCDD
cd04167Snu114pSnu114_GTP-bdCDD
cd16264snRNP_IIICDD
PS51722G_TR_2T_Tr_GTP-bd_domPROSITE profiles
SM00838EFG_CEFG_V-likeSMART
SM00889EFG_IVTransl_elong_EFG/EF2_IVSMART
PR00315ELONGATNFCTT_Tr_GTP-bd_domPRINTS
G3DSA:2.40.30.10:FF:000029CATH-FunFam
G3DSA:3.30.230.10:FF:000009CATH-FunFam
G3DSA:3.30.70.240:FF:000004CATH-FunFam
G3DSA:3.30.70.870:FF:000002CATH-FunFam
G3DSA:3.40.50.300:FF:000646CATH-FunFam
G3DSA:3.90.1430.10:FF:000001CATH-FunFam
G3DSA:2.40.30.10CATH-Gene3D
G3DSA:3.30.230.10Ribsml_uS5_D2-typ_fold_subgrCATH-Gene3D
G3DSA:3.30.70.240CATH-Gene3D
G3DSA:3.30.70.870CATH-Gene3D
G3DSA:3.40.50.300P-loop_NTPaseCATH-Gene3D
G3DSA:3.90.1430.10CATH-Gene3D
SSF50447Transl_B-barrel_sfSUPERFAMILY
SSF52540P-loop_NTPaseSUPERFAMILY
SSF54211Ribosomal_Su5_D2-typ_SFSUPERFAMILY
SSF54980EFG_III/VSUPERFAMILY
PTHR42908PANTHER
TIGR00231small_GTPSmall_GTP-bdNCBIFAM
mobidb-lite-Disorderdisorder_predictionMobiDB-Disorder

Orthologs

References / Literature

PMID:28446597 - The histone variant H2A.Z promotes splicing of weak introns.
Nissen KE et al. Genes Dev 2017 Apr 01;31(7):688-701
PMID:24874881 - The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.
Kallgren SP et al. PLoS Genet 2014;10(5):e1004334
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:24014766 - Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.
Livesay SB et al. Eukaryot Cell 2013 Nov;12(11):1472-89
PMID:18948543 - Splicing factors facilitate RNAi-directed silencing in fission yeast.
Bayne EH et al. Science 2008 Oct 24;322(5901):602-6
PMID:19371376 - Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.
Hanyu Y et al. Genes Cells 2009 May;14(5):539-54
PMID:24442611 - Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes.
Chen W et al. RNA 2014 Mar;20(3):308-20
PMID:25274039 - A systematic genetic screen identifies new factors influencing centromeric heterochromatin integrity in fission yeast.
Bayne EH et al. Genome Biol 2014;15(10):481
PMID:26292707 - Structure of a yeast spliceosome at 3.6-angstrom resolution.
Yan C et al. Science 2015 Sep 11;349(6253):1182-91
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:32415063 - Conserved protein Pir2 ARS2 mediates gene repression through cryptic introns in lncRNAs.
Thillainadesan G et al. Nat Commun 2020 May 15;11(1):2412
PMID:21386897 - Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19.
Ren L et al. PLoS One 2011 Feb 28;6(2):e16719
PMID:39747188 - PhpC NF-Y transcription factor infiltrates heterochromatin to generate cryptic intron-containing transcripts crucial for small RNA production.
Srivastav MK et al. Nat Commun 2025 Jan 02;16(1):268
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:30810475 - Early splicing functions of fission yeast Prp16 and its unexpected requirement for gene Silencing is governed by intronic features.
Vijayakumari D et al. RNA Biol 2019 Jun;16(6):754-769
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:25245948 - Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.
Wang J et al. Nucleic Acids Res 2014 Oct;42(18):11419-32
PMID:39833470 - Structures of aberrant spliceosome intermediates on their way to disassembly.
Soni K et al. Nat Struct Mol Biol 2025 Jan 20;
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74
PMID:22681890 - Hierarchical modularity and the evolution of genetic interactomes across species.
Ryan CJ et al. Mol Cell 2012 Jun 08;46(5):691-704
PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
GO_REF:0000024 - Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
PMID:26682798 - The spliceosome-associated protein Nrl1 suppresses homologous recombination-dependent R-loop formation in fission yeast.
Aronica L et al. Nucleic Acids Res 2016 Feb 29;44(4):1703-17
PMID:36361590 - Defining the Functional Interactome of Spliceosome-Associated G-Patch Protein Gpl1 in the Fission Yeast Schizosaccharomyces pombe .
Selicky T et al. Int J Mol Sci 2022 Oct 24;23(21)
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:42691030 - The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe.
Karika LO et al. PLoS One 2026;21(9):e0357276
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:24081329 - Poly(A) tail-mediated gene regulation by opposing roles of Nab2 and Pab2 nuclear poly(A)-binding proteins in pre-mRNA decay.
Grenier St-Sauveur V et al. Mol Cell Biol 2013 Dec;33(23):4718-31
PMID:26537787 - Targeting of SUMO substrates to a Cdc48-Ufd1-Npl4 segregase and STUbL pathway in fission yeast.
Køhler JB et al. Nat Commun 2015 Nov 05;6:8827
PMID:11884590 - Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.
Ohi MD et al. Mol Cell Biol 2002 Apr;22(7):2011-24
PMID:15755920 - Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex.
Carnahan RH et al. Eukaryot Cell 2005 Mar;4(3):577-87
PMID:28947618 - Sde2 is an intron-specific pre-mRNA splicing regulator activated by ubiquitin-like processing.
Thakran P et al. EMBO J 2018 Jan 04;37(1):89-101
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:42420319 - Spliceosomal proteins direct RNA methylation to modulate gene expression and silence retrotransposons.
Vijayakumari D et al. Nat Commun 2026 Jul 08;
PMID:31219728 - Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe .
Cipakova I et al. Cell Cycle 2019 Jul;18(14):1532-1536
PMID:10409726 - Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing.
McDonald WH et al. Mol Cell Biol 1999 Aug;19(8):5352-62