PomBase home

protein coding gene - num1 (SPBC216.02) - meiotic cortical anchoring factor for dynein Mcp5/Num1

Gene summary

Standard name
num1
Systematic ID
SPBC216.02
Product
meiotic cortical anchoring factor for dynein Mcp5/Num1
Organism
Schizosaccharomyces pombe (fission yeast)
Synonyms
mcp5, mug21
UniProt ID
Q9Y7J9
ORFeome ID
47/47D08
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 898056..901363 forward strand

Annotation

GO biological process

GO:0034643 - establishment of mitochondrion localization, microtubule-mediated

References:

GO:0032118 - horsetail-astral microtubule organization

References:

GO:0032065 - maintenance of protein location in cell cortex

References:

GO:0051646 - mitochondrion localization

References:

GO cellular component

GO:0005938 - cell cortex

References:

GO:0032473 - cytoplasmic side of mitochondrial outer membrane

References:

GO:0009898 - cytoplasmic side of plasma membrane

References:

GO:0106186 - cytoplasmic side of plasma membrane, cell tip

References:

GO:0031520 - plasma membrane of cell tip

References:

GO molecular function

GO:1901612 - cardiolipin binding

References:

GO:0106006 - cytoskeletal protein-membrane anchor activity

References:

GO:0140443 - mitochondrion-plasma membrane adaptor activity

References:

GO:0070300 - phosphatidic acid binding

References:

GO:0005546 - phosphatidylinositol-4,5-bisphosphate binding

References:

Modification

MOD:00006 - N-glycosylated residue

References:

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

References:

Multi-locus phenotype

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0007166 - abolished protein localization to cell cortex during meiotic prophase I

References:

Genotypes:

FYPO:0000903 - decreased rate of microtubule depolymerization during vegetative growth

References:

Genotypes:

Protein features

PBO:0111759 - pleckstrin homology domain

Qualitative gene expression

PomGeneEx:0000018 - protein level increased

References:

PomGeneEx:0000011 - RNA level increased

References:

Quantitative gene expression

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0003024 - abnormal homologous chromosome pairing

References:

Genotypes:

FYPO:0000197 - abnormal horsetail movement

References:

Genotypes:

FYPO:0005990 - abnormal maintenance of protein location in cell cortex of cell tip during karyogamy

References:

Genotypes:

FYPO:0000151 - abnormal meiotic chromosome segregation

References:

Genotypes:

FYPO:0007275 - abnormal mitochondrion inheritance during meiotic cell cycle

References:

Genotypes:

FYPO:0000928 - abnormal protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0003066 - abnormal sporulation resulting in formation of ascus with fewer than four spores

References:

Genotypes:

FYPO:0000927 - abolished horsetail movement

References:

Genotypes:

FYPO:0007168 - abolished microtubule attachment to cell cortex

References:

Genotypes:

FYPO:0007166 - abolished protein localization to cell cortex during meiotic prophase I

References:

Genotypes:

FYPO:0000930 - abolished protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0007164 - decreased microtubule sliding

References:

Genotypes:

FYPO:0007162 - increased duration of nuclear fusion during mating

References:

Genotypes:

FYPO:0004557 - increased vegetative cell population growth

References:

Genotypes:

FYPO:0007171 - normal cell cortex morphology during meiotic prophase I

References:

Genotypes:

FYPO:0000760 - normal mating

References:

Genotypes:

FYPO:0007167 - normal protein localization to cell cortex during meiotic prophase I

References:

Genotypes:

FYPO:0001402 - normal protein localization to cell cortex during vegetative growth

References:

Genotypes:

FYPO:0009038 - resistance to egtazic acid

References:

Genotypes:

FYPO:0001453 - resistance to ethanol

References:

Genotypes:

FYPO:0009085 - resistance to lithium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0009043 - resistance to potassium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0003383 - resistance to tert-butyl hydroperoxide

References:

Genotypes:

FYPO:0001719 - sensitive to lithium

References:

Genotypes:

FYPO:0009088 - sensitive to magnesium chloride and sodium dodecyl sulfate

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011063 - conserved in fungi only

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF12814Mcp5_PHPH_dom-Mcp5-typePFAM
PS50003PH_DOMAINPH_domainPROSITE_PROFILES
SSF50729PH domain-likeSUPERFAMILY
G3DSA:2.30.29.30PH-like_dom_sfGENE3D
CoilCoilCOILS

Orthologs

References / Literature

PMID:28292899 - Fission yeast myosin I facilitates PI(4,5)P 2 -mediated anchoring of cytoplasmic dynein to the cortex.
Thankachan JM et al. Proc Natl Acad Sci U S A 2017 Mar 28;114(13):E2672-E2681
PMID:16585273 - Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast.
Saito TT et al. J Cell Biol 2006 Apr 10;173(1):27-33
PMID:28765164 - Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation.
Touat-Todeschini L et al. EMBO J 2017 Sep 01;36(17):2626-2641
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:26942678 - Enhancer of Rudimentary Cooperates with Conserved RNA-Processing Factors to Promote Meiotic mRNA Decay and Facultative Heterochromatin Assembly.
Sugiyama T et al. Mol Cell 2016 Mar 03;61(5):747-759
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:27984744 - Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.
Joh RI et al. Mol Cell 2016 Dec 15;64(6):1088-1101
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:26670050 - Regulation of mRNA Levels by Decay-Promoting Introns that Recruit the Exosome Specificity Factor Mmi1.
Kilchert C et al. Cell Rep 2015 Dec 22;13(11):2504-2515
PMID:31582398 - Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance.
Chacko LA et al. J Cell Biol 2019 Nov 04;218(11):3560-3571
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:36002457 - Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex.
Foucher AE et al. Nat Commun 2022 Aug 24;13(1):4969
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:30649994 - A conserved mechanism for mitochondria-dependent dynein anchoring.
Kraft LM et al. Mol Biol Cell 2019 Mar 01;30(5):691-702
PMID:24210919 - Mtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance.
Lee NN et al. Cell 2013 Nov 21;155(5):1061-74
PMID:22633491 - Mapping N-glycosylation sites across seven evolutionarily distant species reveals a divergent substrate proteome despite a common core machinery.
Zielinska DF et al. Mol Cell 2012 May 25;46(4):542-8
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:29259000 - Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen.
Blyth J et al. Genetics 2018 Feb;208(2):589-603
PMID:16303567 - A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events.
Martín-Castellanos C et al. Curr Biol 2005 Nov 22;15(22):2056-62
PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
Rodríguez-López M et al. Elife 2023 Oct 03;12
PMID:21317872 - Red1 promotes the elimination of meiosis-specific mRNAs in vegetatively growing fission yeast.
Sugiyama T et al. EMBO J 2011 Mar 16;30(6):1027-39
PMID:33536434 - A scaffold lncRNA shapes the mitosis to meiosis switch.
Andric V et al. Nat Commun 2021 Feb 03;12(1):770
GO_REF:0000002 - Comments
PMID:39705284 - Proteomic and phosphoproteomic analyses reveal that TORC1 is reactivated by pheromone signaling during sexual reproduction in fission yeast.
Bérard M et al. PLoS Biol 2024 Dec 20;22(12):e3002963
PMID:25736293 - Dynactin and Num1 cooperate to establish the cortical anchoring of cytoplasmic dynein in S. pombe.
Fujita I et al. J Cell Sci 2015 Apr 15;128(8):1555-67
PMID:12161753 - The transcriptional program of meiosis and sporulation in fission yeast.
Mata J et al. Nat Genet 2002 Sep;32(1):143-7
PMID:16615890 - Meiotic proteins bqt1 and bqt2 tether telomeres to form the bouquet arrangement of chromosomes.
Chikashige Y et al. Cell 2006 Apr 07;125(1):59-69
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:40185772 - A bifunctional snoRNA with separable activities in guiding rRNA 2'-O-methylation and scaffolding gametogenesis effectors.
Leroy E et al. Nat Commun 2025 Apr 05;16(1):3250
PMID:28841135 - Ubiquitination-dependent control of sexual differentiation in fission yeast.
Simonetti F et al. Elife 2017 Aug 25;6
PMID:25375137 - Systematic analysis of the role of RNA-binding proteins in the regulation of RNA stability.
Hasan A et al. PLoS Genet 2014 Nov;10(11):e1004684
PMID:36882296 - Duf89 abets lncRNA control of fission yeast phosphate homeostasis via its antagonism of precocious lncRNA transcription termination.
Sanchez AM et al. RNA 2023 Jun;29(6):808-825
PMID:24095277 - Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.
Marasovic M et al. Mol Cell 2013 Oct 24;52(2):173-83
PMID:23658229 - Red5 and three nuclear pore components are essential for efficient suppression of specific mRNAs during vegetative growth of fission yeast.
Sugiyama T et al. Nucleic Acids Res 2013 Jul;41(13):6674-86
PMID:16624923 - Fission yeast Num1p is a cortical factor anchoring dynein and is essential for the horse-tail nuclear movement during meiotic prophase.
Yamashita A et al. Genetics 2006 Jul;173(3):1187-96