protein coding gene - swi1 (SPBC216.06c) - replication fork protection complex subunit Swi1
Gene summary
- Standard name
- swi1
- Systematic ID
- SPBC216.06c
- Product
- replication fork protection complex subunit Swi1
- Organism
- Schizosaccharomyces pombe
(fission yeast)
- UniProt ID
- Q9UUM2
- ORFeome ID
- 47/47F08
- Characterisation status
- biological role published
- Feature type
- mRNA gene
- Genomic location
- chromosome II: 907370..914779 reverse strand
Annotation
Multi-locus phenotype
References:
Genotypes:
- cdc23-M36(D232G,V265I aa) swi1-1(disruption)
- cdc23-M36(D232G,V265I aa) swi1-111(unknown)
- hhf1-KR(K6R,K9R,K13R,K17R aa) swi1delta
- hhf2-KR(K6R,K9R,K13R,K17R aa) swi1delta
- hsk1-89(L339P,E403K,L416P aa) swi1delta
- mus81delta rad52delta swi1delta
- rad26delta swi1delta
- rad51delta swi1delta
- rad52delta swi1delta
- rad54delta swi1delta
- rqh1delta swi1delta
- srs2delta swi1delta
Qualitative gene expression
Quantitative gene expression
Protein features
| ID | Name | InterPro name | DB name |
| PF04821 | TIMELESS | Timeless_N | Pfam |
| PF05029 | TIMELESS_C | TIMELESS_PAB | Pfam |
| PF27570 | TOF-1-like_helical | TOF-1-like_helical | Pfam |
| PTHR22940 | | Timeless | PANTHER |
| Coil | | | COILS |
Orthologs
- TIMELESS (HGNC:11813) Homo sapiens
- swi1 (SJAG_02724) Schizosaccharomyces japonicus
- TOF1 (YNL273W) Saccharomyces cerevisiae
References / Literature
- PMID:19037101 - Mus81, Rhp51(Rad51), and Rqh1 form an epistatic pathway required for the S-phase DNA damage checkpoint.
- Willis N et al. Mol Biol Cell 2009 Feb;20(3):819-33
- PMID:23071723 - DNA polymerase α (swi7) and the flap endonuclease Fen1 (rad2) act together in the S-phase alkylation damage response in S. pombe.
- Koulintchenko M et al. PLoS One 2012;7(10):e47091
- PMID:12840005 - Complex mechanism of site-specific DNA replication termination in fission yeast.
- Codlin S et al. EMBO J 2003 Jul 01;22(13):3431-40
- PMID:14560029 - Swi1 prevents replication fork collapse and controls checkpoint kinase Cds1.
- Noguchi E et al. Mol Cell Biol 2003 Nov;23(21):7861-74
- PMID:26990647 - Swi1Timeless Prevents Repeat Instability at Fission Yeast Telomeres.
- Gadaleta MC et al. PLoS Genet 2016 Mar;12(3):e1005943
- PMID:20885790 - Critical functions of Rpa3/Ssb3 in S-phase DNA damage responses in fission yeast.
- Cavero S et al. PLoS Genet 2010 Sep 23;6(9):e1001138
- PMID:25965521 - Ku stabilizes replication forks in the absence of Brc1.
- Sánchez A et al. PLoS One 2015;10(5):e0126598
- PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
- Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
- PMID:20967229 - Checkpoint-dependent and -independent roles of Swi3 in replication fork recovery and sister chromatid cohesion in fission yeast.
- Rapp JB et al. PLoS One 2010 Oct 12;5(10):e13379
- PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
- Hayles J et al. Open Biol 2013 May 22;3(5):130053
- PMID:30148840 - Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks.
- Ganguly A et al. PLoS Genet 2018 Aug;14(8):e1007595
- PMID:30759238 - Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.
- Singh B et al. Nucleic Acids Res 2019 Apr 23;47(7):3422-3433
- PMID:32372157 - Characterisation of unessential genes required for survival under conditions of DNA stress.
- Ahmed Ezzat H et al. J Genet Eng Biotechnol 2020 May 06;18(1):14
- PMID:21183410 - Schizosaccharomyces pombe Mms1 channels repair of perturbed replication into Rhp51 independent homologous recombination.
- Vejrup-Hansen R et al. DNA Repair (Amst) 2011 Mar 07;10(3):283-95
- PB_REF:0000006 - Disease associations from Monarch via human-pombe orthologs
-
- PMID:19547744 - Evolution of phosphoregulation: comparison of phosphorylation patterns across yeast species.
- Beltrao P et al. PLoS Biol 2009 Jun 16;7(6):e1000134
- PMID:19273851 - Recombination at DNA replication fork barriers is not universal and is differentially regulated by Swi1.
- Pryce DW et al. Proc Natl Acad Sci U S A 2009 Mar 24;106(12):4770-5
- PMID:19205745 - Redundant roles of Srs2 helicase and replication checkpoint in survival and rDNA maintenance in Schizosaccharomyces pombe.
- Yasuhira S Mol Genet Genomics 2009 May;281(5):497-509
- PMID:19422421 - Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeast.
- Shimmoto M et al. Genes Cells 2009 Jun;14(6):669-82
- PMID:20065069 - The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.
- Willis N et al. Genetics 2010 Apr;184(4):887-97
- PMID:17189249 - Tel2 is required for activation of the Mrc1-mediated replication checkpoint.
- Shikata M et al. J Biol Chem 2007 Feb 23;282(8):5346-55
- PMID:18045993 - RFCCtf18 and the Swi1-Swi3 complex function in separate and redundant pathways required for the stabilization of replication forks to facilitate sister chromatid cohesion in Schizosaccharomyces pombe.
- Ansbach AB et al. Mol Biol Cell 2008 Feb;19(2):595-607
- PMID:18667534 - Activation of the DNA damage checkpoint in mutants defective in DNA replication initiation.
- Yin L et al. Mol Biol Cell 2008 Oct;19(10):4374-82
- PMID:30355493 - Expanded Interactome of the Intrinsically Disordered Protein Dss1.
- Schenstrøm SM et al. Cell Rep 2018 Oct 23;25(4):862-870
- PMID:11030618 - swi1 and swi3 perform imprinting, pausing, and termination of DNA replication in S. pombe.
- Dalgaard JZ et al. Cell 2000 Sep 15;102(6):745-51
- PMID:22426534 - DNA replication through hard-to-replicate sites, including both highly transcribed RNA Pol II and Pol III genes, requires the S. pombe Pfh1 helicase.
- Sabouri N et al. Genes Dev 2012 Mar 15;26(6):581-93
- PMID:22645654 - Opposing role of condensin hinge against replication protein A in mitosis and interphase through promoting DNA annealing.
- Akai Y et al. Open Biol 2011 Dec;1(4):110023
- PMID:27473316 - Characterization of a Novel MMS-Sensitive Allele of Schizosaccharomyces pombe mcm4.
- Ranatunga NS et al. G3 (Bethesda) 2016 Oct 13;6(10):3049-3063
- PMID:20924116 - Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1.
- Tanaka T et al. J Biol Chem 2010 Dec 17;285(51):39609-22
- PMID:24189946 - Gene activation by copy transposition in mating-type switching of a homothallic fission yeast.
- Egel R et al. Curr Genet 1981 Apr;3(1):5-12
- PMID:32355220 - DNA replication machinery prevents Rad52-dependent single-strand annealing that leads to gross chromosomal rearrangements at centromeres.
- Onaka AT et al. Commun Biol 2020 Apr 30;3(1):202
- PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
- Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
- PMID:21099360 - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeast.
- Matsumoto S et al. Cell Cycle 2010 Dec 01;9(23):4627-37
- PMID:30992049 - The NuA4 acetyltransferase and histone H4 acetylation promote replication recovery after topoisomerase I-poisoning.
- Noguchi C et al. Epigenetics Chromatin 2019 Apr 16;12(1):24
- PMID:20176980 - Fission yeast Hsk1 (Cdc7) kinase is required after replication initiation for induced mutagenesis and proper response to DNA alkylation damage.
- Dolan WP et al. Genetics 2010 May;185(1):39-53
- PMID:37787768 - Broad functional profiling of fission yeast proteins using phenomics and machine learning.
- Rodríguez-López M et al. Elife 2023 Oct 03;12
- PMID:31626996 - Multiplexed proteome profiling of carbon source perturbations in two yeast species with SL-SP3-TMT.
- Paulo JA et al. J Proteomics 2020 Jan 06;210:103531
- PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
- Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
- PMID:11387218 - A role for DNA polymerase alpha in epigenetic control of transcriptional silencing in fission yeast.
- Nakayama Ji et al. EMBO J 2001 Jun 01;20(11):2857-66
- PMID:29610759 - Molecular signature of the imprintosome complex at the mating-type locus in fission yeast.
- Raimondi C et al. Microb Cell 2018 Jan 16;5(4):169-183
- PMID:22927644 - Replication fork collapse and genome instability in a deoxycytidylate deaminase mutant.
- Sánchez A et al. Mol Cell Biol 2012 Nov;32(21):4445-54
- PMID:21511999 - Comparative functional genomics of the fission yeasts.
- Rhind N et al. Science 2011 May 20;332(6032):930-6
- PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
- Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
- PMID:15509785 - Biochemical interactions between proteins and mat1 cis-acting sequences required for imprinting in fission yeast.
- Lee BS et al. Mol Cell Biol 2004 Nov;24(22):9813-22
- PMID:37615341 - Schizosaccharomyces pombe Rtf2 is important for replication fork barrier activity of RTS1 via splicing of Rtf1 .
- Budden AM et al. Elife 2023 Aug 24;12
- PMID:15367656 - Swi1 and Swi3 are components of a replication fork protection complex in fission yeast.
- Noguchi E et al. Mol Cell Biol 2004 Oct;24(19):8342-55
- PMID:27068713 - Timeless protection of telomeres.
- Gadaleta MC et al. Curr Genet 2016 Nov;62(4):725-730
- PMID:24013502 - Epe1 recruits BET family bromodomain protein Bdf2 to establish heterochromatin boundaries.
- Wang J et al. Genes Dev 2013 Sep 01;27(17):1886-902
- PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
- Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
- PMID:23260662 - Lsd1 and lsd2 control programmed replication fork pauses and imprinting in fission yeast.
- Holmes A et al. Cell Rep 2012 Dec 27;2(6):1513-20
- PMID:22095079 - The double-bromodomain proteins Bdf1 and Bdf2 modulate chromatin structure to regulate S-phase stress response in Schizosaccharomyces pombe.
- Garabedian MV et al. Genetics 2012 Feb;190(2):487-500
- PMID:3481026 - Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching.
- Schmidt H Mol Gen Genet 1987 Dec;210(3):485-9
- PMID:6587363 - Genes required for initiation and resolution steps of mating-type switching in fission yeast.
- Egel R et al. Proc Natl Acad Sci U S A 1984 Jun;81(11):3481-5
- PMID:17660542 - Mms22 preserves genomic integrity during DNA replication in Schizosaccharomyces pombe.
- Dovey CL et al. Genetics 2007 Sep;177(1):47-61
- PMID:27687866 - Genetic controls of DNA damage avoidance in response to acetaldehyde in fission yeast.
- Noguchi C et al. Cell Cycle 2017 Jan 02;16(1):45-58
- PMID:15767681 - Schizosaccharomyces pombe Swi1, Swi3, and Hsk1 are components of a novel S-phase response pathway to alkylation damage.
- Sommariva E et al. Mol Cell Biol 2005 Apr;25(7):2770-84
- PMID:21215368 - The Cul4-Ddb1(Cdt)² ubiquitin ligase inhibits invasion of a boundary-associated antisilencing factor into heterochromatin.
- Braun S et al. Cell 2011 Jan 07;144(1):41-54
- PMID:20118936 - Schizosaccharomyces pombe genome-wide nucleosome mapping reveals positioning mechanisms distinct from those of Saccharomyces cerevisiae.
- Lantermann AB et al. Nat Struct Mol Biol 2010 Feb;17(2):251-7
- PMID:17151242 - Sap1 promotes the association of the replication fork protection complex with chromatin and is involved in the replication checkpoint in Schizosaccharomyces pombe.
- Noguchi C et al. Genetics 2007 Feb;175(2):553-66
- PMID:39094566 - Mrc1 regulates parental histone segregation and heterochromatin inheritance.
- Toda T et al. Mol Cell 2024 Jul 23;
- PMID:22952839 - Swi1 associates with chromatin through the DDT domain and recruits Swi3 to preserve genomic integrity.
- Noguchi C et al. PLoS One 2012;7(8):e43988
- PMID:16263721 - Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complex.
- Matsumoto S et al. J Biol Chem 2005 Dec 30;280(52):42536-42
- PMID:21518960 - Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeast.
- Hayano M et al. Mol Cell Biol 2011 Jun;31(12):2380-91
- PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
- Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
- PMID:23163955 - Analysis of stress-induced duplex destabilization (SIDD) properties of replication origins, genes and intergenes in the fission yeast, Schizosaccharomyces pombe.
- Yadav MP et al. BMC Res Notes 2012 Nov 19;5:643
- PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
- Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
- GO_REF:0000033 - Annotation inferences using phylogenetic trees
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- PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
- Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87
- PMID:26201080 - The DNA-Binding Domain of S. pombe Mrc1 (Claspin) Acts to Enhance Stalling at Replication Barriers.
- Zech J et al. PLoS One 2015;10(7):e0132595
- PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
- Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
- PMID:10716938 - Fission yeast switches mating type by a replication-recombination coupled process.
- Arcangioli B et al. EMBO J 2000 Mar 15;19(6):1389-96
- PMID:23349636 - Coordinated degradation of replisome components ensures genome stability upon replication stress in the absence of the replication fork protection complex.
- Roseaulin LC et al. PLoS Genet 2013;9(1):e1003213
- PMID:23703609 - TORC2 is required to maintain genome stability during S phase in fission yeast.
- Schonbrun M et al. J Biol Chem 2013 Jul 05;288(27):19649-60
- PMID:24875629 - Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.
- Ding L et al. G3 (Bethesda) 2014 May 28;4(8):1373-84
- PMID:34608864 - DDK/Hsk1 phosphorylates and targets fission yeast histone deacetylase Hst4 for degradation to stabilize stalled DNA replication forks.
- Aricthota S et al. Elife 2021 Oct 05;10
- PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
- Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
- PMID:24719968 - Switching genes in Schizosaccharomyces pombe.
- Gutz H et al. Curr Genet 1985;9(5):325-31
- PMID:16710300 - Sws1 is a conserved regulator of homologous recombination in eukaryotic cells.
- Martín V et al. EMBO J 2006 Jun 07;25(11):2564-74
- PMID:18723894 - Rtf1-mediated eukaryotic site-specific replication termination.
- Eydmann T et al. Genetics 2008 Sep;180(1):27-39
- PMID:17209013 - Role of SUMO in the dynamics of telomere maintenance in fission yeast.
- Xhemalce B et al. Proc Natl Acad Sci U S A 2007 Jan 16;104(3):893-8
- PMID:18257517 - Phosphoproteome analysis of fission yeast.
- Wilson-Grady JT et al. J Proteome Res 2008 Mar;7(3):1088-97
- PMID:32034465 - Genetic investigation of formaldehyde-induced DNA damage response in Schizosaccharomyces pombe.
- Anandarajan V et al. Curr Genet 2020 Jun;66(3):593-605
- PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
- Halova L et al. Open Biol 2021 Apr;11(4):200405
- PMID:41873186 - Homologous recombination mutants cause differing lethality between h- and h+ Schizosaccharomyces pombe strains due to mat1 heterochromatin.
- Kolesar P et al. FEBS J 2026 Mar 23;
- PMID:20661445 - Rad3 decorates critical chromosomal domains with gammaH2A to protect genome integrity during S-Phase in fission yeast.
- Rozenzhak S et al. PLoS Genet 2010 Jul 22;6(7):e1001032
