PomBase home

protein coding gene - spt4 (SPBC21C3.16c) - DSIF transcription elongation factor complex subunit Spt4

Gene summary

Standard name
spt4
Systematic ID
SPBC21C3.16c
Product
DSIF transcription elongation factor complex subunit Spt4
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
Q9P7K8
ORFeome ID
01/01F09
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3824636..3824994 reverse strand

Annotation

Complementation

PBO:0004683 - functionally complements S. cerevisiae Spt4

References:

GO biological process

GO:0006368 - transcription elongation by RNA polymerase II

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0032044 - DSIF complex

References:

GO:0005634 - nucleus

References:

GO molecular function

GO:0005515 - protein binding

References:

GO:0000993 - RNA polymerase II complex binding

References:

GO:0003711 - transcription elongation factor activity

References:

Modification

MOD:01148 - ubiquitinylated lysine

References:

Multi-locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

Protein sequence feature

SO:0000418 - signal_peptide

References:

Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000082 - decreased cell population growth at high temperature

References:

Genotypes:

FYPO:0001407 - decreased cell population growth on glucose carbon source

References:

Genotypes:

FYPO:0000470 - decreased mating type switching

References:

Genotypes:

FYPO:0001740 - increased gross chromosomal rearrangement

References:

Genotypes:

FYPO:0000674 - normal cell population growth at high temperature

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

FYPO:0002177 - viable vegetative cell with normal cell morphology

References:

Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF06093Spt4Spt4/RpoE2_ZnfPFAM
cd07973Spt4Spt4/RpoE2_ZnfCDD
SM01389Spt4_2Spt4/RpoE2_ZnfSMART
G3DSA:3.30.40.210:FF:000003FUNFAM
SSF63393RNA polymerase subunitsRPABC4/Spt4SUPERFAMILY
G3DSA:3.30.40.210Spt4_sfGENE3D
PTHR12882SUPPRESSOR OF TY 4Spt4PANTHER
PIRSF025023Spt4Spt4PIRSF

Orthologs

References / Literature

PMID:29852001 - New insights into donor directionality of mating-type switching in Schizosaccharomyces pombe.
Maki T et al. PLoS Genet 2018 May;14(5):e1007424
PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:11893740 - Interactions between fission yeast mRNA capping enzymes and elongation factor Spt5.
Pei Y et al. J Biol Chem 2002 May 31;277(22):19639-48
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16914721 - Upf1, an RNA helicase required for nonsense-mediated mRNA decay, modulates the transcriptional response to oxidative stress in fission yeast.
Rodríguez-Gabriel MA et al. Mol Cell Biol 2006 Sep;26(17):6347-56
PMID:34980915 - SignalP 6.0 predicts all five types of signal peptides using protein language models.
Teufel F et al. Nat Biotechnol 2022 Jul;40(7):1023-1025
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:19460865 - Characterization of the Schizosaccharomyces pombe Spt5-Spt4 complex.
Schwer B et al. RNA 2009 Jul;15(7):1241-50
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
GO_REF:0000033 - Annotation inferences using phylogenetic trees
PMID:28218250 - Chromatin remodeller Fun30 Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation.
Lee J et al. Nat Commun 2017 Feb 20;8:14527
PMID:30652128 - Heterochromatin suppresses gross chromosomal rearrangements at centromeres by repressing Tfs1/TFIIS-dependent transcription.
Okita AK et al. Commun Biol 2019;2:17
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053