protein coding gene - sid4 (SPBC244.01c) - SIN component scaffold protein Sid4

Gene summary

Standard name

sid4

Systematic ID

SPBC244.01c

Product

SIN component scaffold protein Sid4

Organism

Schizosaccharomyces pombe (fission yeast)

UniProt ID

O60187

ORFeome ID

37/37H11

Characterisation status

biological role published

Feature type

mRNA gene

Genomic location

chromosome II: 4360626..4363926 forward strand

Annotation

Protein features

Orthologs

• sid4 (SJAG_01464) Schizosaccharomyces japonicus
• ADY3 (YDL239C) Saccharomyces cerevisiae
• CNM67 (YNL225C) Saccharomyces cerevisiae

References / Literature

PMID:37783794 - An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity.

Ader NR et al. Nat Cell Biol 2023 Oct;25(10):1465-1477

PMID:18411246 - Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation.

Ohtaka A et al. J Cell Sci 2008 May 01;121(Pt 9):1547-58

PMID:16775007 - Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body.

Rosenberg JA et al. Mol Biol Cell 2006 Sep;17(9):3793-805

PMID:24790095 - Characterization of the roles of Blt1p in fission yeast cytokinesis.

Goss JW et al. Mol Biol Cell 2014 Jul 01;25(13):1946-57

PMID:10545452 - Drc1p/Cps1p, a 1,3-beta-glucan synthase subunit, is essential for division septum assembly in Schizosaccharomyces pombe.

Liu J et al. Genetics 1999 Nov;153(3):1193-203

PMID:18562696 - The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast.

Yan H et al. Mol Biol Cell 2008 Sep;19(9):3676-90

PMID:24838944 - Dma1-dependent degradation of SIN proteins during meiosis in Schizosaccharomyces pombe.

Krapp A et al. J Cell Sci 2014 Jul 15;127(Pt 14):3149-61

PMID:10799520 - Byr4 localizes to spindle-pole bodies in a cell cycle-regulated manner to control Cdc7 localization and septation in fission yeast.

Li C et al. J Biol Chem 2000 May 12;275(19):14381-7

PMID:39476757 - Characterization of Ksg1 protein kinase-dependent phosphoproteome in the fission yeast S. pombe.

Cipak L et al. Biochem Biophys Res Commun 2024 Oct 25;736:150895

PMID:24055157 - CK1 is required for a mitotic checkpoint that delays cytokinesis.

Johnson AE et al. Curr Biol 2013 Oct 07;23(19):1920-6

PMID:10459013 - Sid2p, a spindle pole body kinase that regulates the onset of cytokinesis.

Sparks CA et al. J Cell Biol 1999 Aug 23;146(4):777-90

PMID:16415366 - A role for the septation initiation network in septum assembly revealed by genetic analysis of sid2-250 suppressors.

Jin QW et al. Genetics 2006 Apr;172(4):2101-12

PMID:9649519 - Isolation and characterization of new fission yeast cytokinesis mutants.

Balasubramanian MK et al. Genetics 1998 Jul;149(3):1265-75

PMID:39471327 - New mutations in the core Schizosaccharomyces pombe spindle pole body scaffold Ppc89 reveal separable functions in regulating cell division.

Hanna SM et al. G3 (Bethesda) 2024 Oct 29;

PMID:10805785 - Sid4p is required to localize components of the septation initiation pathway to the spindle pole body in fission yeast.

Chang L et al. Proc Natl Acad Sci U S A 2000 May 09;97(10):5249-54

PMID:20603077 - Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.

Roberts-Galbraith RH et al. Mol Cell 2010 Jul 09;39(1):86-99

PMID:11514436 - Correct regulation of the septation initiation network in Schizosaccharomyces pombe requires the activities of par1 and par2.

Jiang W et al. Genetics 2001 Aug;158(4):1413-29

PMID:20980623 - A novel role of Dma1 in regulating forespore membrane assembly and sporulation in fission yeast.

Li WZ et al. Mol Biol Cell 2010 Dec;21(24):4349-60

PMID:22669973 - Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function.

Johnson AE et al. J Biol Chem 2012 Jul 27;287(31):25741-8

PMID:22419817 - The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11.

Feoktistova A et al. Mol Biol Cell 2012 May;23(9):1636-45

PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.

Hayles J et al. Open Biol 2013 May 22;3(5):130053

PMID:27889481 - Mitotic Nuclear Envelope Breakdown and Spindle Nucleation Are Controlled by Interphase Contacts between Centromeres and the Nuclear Envelope.

Fernández-Álvarez A et al. Dev Cell 2016 Dec 05;39(5):544-559

PMID:34346498 - Loss of kinesin-8 improves the robustness of the self-assembled spindle in Schizosaccharomyces pombe.

Pineda-Santaella A et al. J Cell Sci 2021 Aug 15;134(16)

PMID:38865179 - Transient PP2A SIP complex localization to mitotic SPBs for SIN inhibition is mediated solely by the Csc1 FHA domain.

Willet AH et al. Mol Biol Cell 2024 Jun 12;:mbcE24040196

PMID:39786922 - The fission yeast SUMO-targeted ubiquitin ligase Slx8 functionally associates with clustered centromeres and the silent mating-type region at the nuclear periphery.

Chakraborty S et al. Biol Open 2024 Dec 15;13(12)

PMID:28774892 - Dialogue between centrosomal entrance and exit scaffold pathways regulates mitotic commitment.

Chan KY et al. J Cell Biol 2017 Sep 04;216(9):2795-2812

PMID:15857958 - Roles of Pdk1p, a fission yeast protein related to phosphoinositide-dependent protein kinase, in the regulation of mitosis and cytokinesis.

Bimbó A et al. Mol Biol Cell 2005 Jul;16(7):3162-75

PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.

Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105

PMID:20624220 - Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast.

Cadou A et al. Mol Microbiol 2010 Sep;77(5):1186-202

PMID:22809626 - Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast.

Wang Y et al. Mol Biol Cell 2012 Sep;23(17):3348-56

PMID:34674264 - Localization of the ubiquitin ligase Dma1 to the fission yeast contractile ring is modulated by phosphorylation.

Chen JS et al. FEBS Lett 2021 Nov;595(22):2781-2792

PMID:11707284 - The protein phosphatase 2A B'-regulatory subunit par1p is implicated in regulation of the S. pombe septation initiation network.

Le Goff X et al. FEBS Lett 2001 Nov 09;508(1):136-42

PMID:38985524 - The core spindle pole body scaffold Ppc89 links the pericentrin ortholog Pcp1 to the fission yeast spindle pole body via an evolutionarily conserved interface.

Chen JS et al. Mol Biol Cell 2024 Jul 10;:mbcE24050220

PMID:28619713 - Molecular model of fission yeast centrosome assembly determined by superresolution imaging.

Bestul AJ et al. J Cell Biol 2017 Aug 07;216(8):2409-2424

PMID:11739799 - Localization of fission yeast type II myosin, Myo2, to the cytokinetic actin ring is regulated by phosphorylation of a C-terminal coiled-coil domain and requires a functional septation initiation network.

Mulvihill DP et al. Mol Biol Cell 2001 Dec;12(12):4044-53

PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.

Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623

PMID:21131906 - Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase.

Johnson AE et al. EMBO J 2011 Jan 19;30(2):341-54

PMID:11676915 - S. pombe cdc11p, together with sid4p, provides an anchor for septation initiation network proteins on the spindle pole body.

Krapp A et al. Curr Biol 2001 Oct 16;11(20):1559-68

PMID:10462526 - The S. pombe zfs1 gene is required to prevent septation if mitotic progression is inhibited.

Beltraminelli N et al. J Cell Sci 1999 Sep;112 Pt 18:3103-14

PMID:20833892 - Schizosaccharomyces pombe calmodulin, Cam1, plays a crucial role in sporulation by recruiting and stabilizing the spindle pole body components responsible for assembly of the forespore membrane.

Itadani A et al. Eukaryot Cell 2010 Dec;9(12):1925-35

PMID:12242222 - The Scw1 RNA-binding domain protein regulates septation and cell-wall structure in fission yeast.

Karagiannis J et al. Genetics 2002 Sep;162(1):45-58

PMID:24530531 - Mcp1p tracks microtubule plus ends to destabilize microtubules at cell tips.

Zheng F et al. FEBS Lett 2014 Mar 18;588(6):859-65

PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.

Marguerat S et al. Cell 2012 Oct 26;151(3):671-83

PMID:30853434 - NDR Kinase Sid2 Drives Anillin-like Mid1 from the Membrane to Promote Cytokinesis and Medial Division Site Placement.

Willet AH et al. Curr Biol 2019 Mar 18;29(6):1055-1063.e2

PMID:11950932 - The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network.

Tomlin GC et al. Mol Biol Cell 2002 Apr;13(4):1203-14

PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.

Strachan J et al. J Cell Sci 2023 Dec 01;136(23)

PMID:19139265 - The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.

Roberts-Galbraith RH et al. J Cell Biol 2009 Jan 12;184(1):113-27

PMID:35099006 - Fission yeast polycystin Pkd2p promotes cell size expansion and antagonizes the Hippo-related SIN pathway.

Sinha D et al. J Cell Sci 2022 Feb 15;135(4)

PMID:10775265 - The role of the sid1p kinase and cdc14p in regulating the onset of cytokinesis in fission yeast.

Guertin DA et al. EMBO J 2000 Apr 17;19(8):1803-15

PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.

Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514

PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.

Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87

PMID:16085489 - The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast.

Mishra M et al. Curr Biol 2005 Aug 09;15(15):1376-83

PMID:40027523 - Characterization and comparison of temperature-sensitive Schizosaccharomyces pombe mutants of the septation initiation network scaffolds, Cdc11 and Sid4.

Turner LA et al. MicroPubl Biol 2025;2025

PMID:21511999 - Comparative functional genomics of the fission yeasts.

Rhind N et al. Science 2011 May 20;332(6032):930-6

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.

Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41

PMID:12546793 - Mitotic hyperphosphorylation of the fission yeast SIN scaffold protein cdc11p is regulated by the protein kinase cdc7p.

Krapp A et al. Curr Biol 2003 Jan 21;13(2):168-72

PMID:11271422 - Bypass of the requirement for cdc16p GAP function in Schizosaccharomyces pombe by mutation of the septation initiation network genes.

Fournier N et al. Arch Microbiol 2001 Jan;175(1):62-9

PMID:20805322 - The mitosis-to-interphase transition is coordinated by cross talk between the SIN and MOR pathways in Schizosaccharomyces pombe.

Ray S et al. J Cell Biol 2010 Sep 06;190(5):793-805

PMID:22119525 - SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast.

Singh NS et al. Curr Biol 2011 Dec 06;21(23):1968-78

PMID:29742018 - The kinase domain of CK1 enzymes contains the localization cue essential for compartmentalized signaling at the spindle pole.

Elmore ZC et al. Mol Biol Cell 2018 Jul 01;29(13):1664-1674

PMID:29975113 - Relief of the Dma1-mediated checkpoint requires Dma1 autoubiquitination and dynamic localization.

Jones CM et al. Mol Biol Cell 2018 Sep 01;29(18):2176-2189

PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7

PMID:36749320 - Multiple polarity kinases inhibit phase separation of F-BAR protein Cdc15 and antagonize cytokinetic ring assembly in fission yeast.

Bhattacharjee R et al. Elife 2023 Feb 07;12

PMID:11683392 - Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis.

Cueille N et al. J Cell Sci 2001 Jul;114(Pt 14):2649-64

PMID:15132994 - Recruitment of NIMA kinase shows that maturation of the S. pombe spindle-pole body occurs over consecutive cell cycles and reveals a role for NIMA in modulating SIN activity.

Grallert A et al. Genes Dev 2004 May 01;18(9):1007-21

PMID:16787941 - The Schizosaccharomyces pombe septation initiation network (SIN) is required for spore formation in meiosis.

Krapp A et al. J Cell Sci 2006 Jul 15;119(Pt 14):2882-91

PMID:10769201 - The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation.

Salimova E et al. J Cell Sci 2000 May;113 ( Pt 10):1695-704

PMID:14655046 - Two-hybrid search for proteins that interact with Sad1 and Kms1, two membrane-bound components of the spindle pole body in fission yeast.

Miki F et al. Mol Genet Genomics 2004 Jan;270(6):449-61

PMID:12479804 - Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN).

Guertin DA et al. Dev Cell 2002 Dec;3(6):779-90

PMID:12796296 - Scw1p antagonizes the septation initiation network to regulate septum formation and cell separation in the fission yeast Schizosaccharomyces pombe.

Jin QW et al. Eukaryot Cell 2003 Jun;2(3):510-20

PMID:15062098 - Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB.

Morrell JL et al. Curr Biol 2004 Apr 06;14(7):579-84

PMID:10837231 - Mob1p interacts with the Sid2p kinase and is required for cytokinesis in fission yeast.

Hou MC et al. Curr Biol 2000 May 18;10(10):619-22