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protein coding gene - mis3 (SPBC25B2.05) - rRNA processing protein Mis3

Gene summary

Standard name
mis3
Systematic ID
SPBC25B2.05
Product
rRNA processing protein Mis3
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O74777
ORFeome ID
32/32C01
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 2605901..2607020 forward strand

Annotation

GO biological process

GO:0006364 - rRNA processing

References:

GO cellular component

GO:0005730 - nucleolus

References:

GO:0005634 - nucleus

References:

GO:0032040 - small-subunit processome

References:

GO molecular function

GO:0003723 - RNA binding

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:00047 - O-phospho-L-threonine

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Multi-locus phenotype

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

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Genotypes:

Protein features

PBO:0111813 - KH domain

Qualitative gene expression

PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

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Single locus phenotype

FYPO:0008218 - abnormal mitotic S/G2 phase transition

References:

Genotypes:

FYPO:0004481 - abolished cell population growth at high temperature

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Genotypes:

FYPO:0003694 - decreased mature 18S rRNA level

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Genotypes:

FYPO:0004194 - decreased protein level during cellular response to hydroxyurea

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Genotypes:

FYPO:0000786 - increased plasmid loss

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Genotypes:

FYPO:0000310 - inviable after spore germination, without cell division, with normal germ tube morphology

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Genotypes:

FYPO:0001490 - inviable elongated vegetative cell

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Genotypes:

FYPO:0002151 - inviable spore

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Genotypes:

FYPO:0002061 - inviable vegetative cell population

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Genotypes:

FYPO:0002273 - inviable vegetative cell with abnormal cell morphology

References:

Genotypes:

FYPO:0004629 - normal mitotic DNA replication

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Genotypes:

FYPO:0001120 - pear-shaped vegetative cell

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Genotypes:

FYPO:0000104 - sensitive to cycloheximide

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Genotypes:

FYPO:0000088 - sensitive to hydroxyurea

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Genotypes:

FYPO:0007136 - small mononucleate vegetative cell

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Genotypes:

FYPO:0000024 - stubby vegetative cell

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Genotypes:

Taxonomic conservation

PBO:0011065 - conserved in eukaryotes

PBO:0011071 - conserved in eukaryotes only

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF21800KH_KRR1_2ndKRR1-like_KH2PFAM
PF17903KH_KRR1_1stKRR1-like_KH1PFAM
cd22394KH-I_KRR1_rpt2KRR1-like_KH2_eukCDD
cd22393KH-I_KRR1_rpt1KRR1-like_KH1_eukCDD
G3DSA:3.30.1370.10:FF:000011FUNFAM
G3DSA:3.30.1370.10:FF:000014FUNFAM
SSF54791Eukaryotic type KH-domain (KH-domain type I)KH_dom_type_1_sfSUPERFAMILY
G3DSA:3.30.1370.10K Homology domain, type 1KH_dom_type_1_sfGENE3D
PTHR12581HIV-1 REV BINDING PROTEIN 2, 3rRNA_assembly_KRR1PANTHER
PIRSF006515KRR1rRNA_assembly_KRR1PIRSF
CoilCoilCOILS
mobidb-lite-Disorderdisorder_predictionMOBIDB-Disorder
mobidb-lite-Polyampholytedisorder_predictionMOBIDB-Polyampholyte

Orthologs

References / Literature

PMID:16823372 - ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Matsuyama A et al. Nat Biotechnol 2006 Jul;24(7):841-7
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:15126629 - Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis.
Kimata Y et al. J Cell Sci 2004 May 01;117(Pt 11):2283-93
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:24713849 - Post-transcriptional regulation of meiotic genes by a nuclear RNA silencing complex.
Egan ED et al. RNA 2014 Jun;20(6):867-81
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:7865880 - Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality.
Takahashi K et al. Mol Biol Cell 1994 Oct;5(10):1145-58
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
GO_REF:0000002 - Comments
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:16537923 - Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast.
Todd BL et al. Mol Cell Biol 2006 Apr;26(7):2817-31
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:10947840 - Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint.
Kondoh H et al. Genes Cells 2000 Jul;5(7):525-41
PMID:30321377 - Proteomic profiling and functional characterization of post-translational modifications of the fission yeast RNA exosome.
Telekawa C et al. Nucleic Acids Res 2018 Nov 30;46(21):11169-11183
PMID:12529438 - Global transcriptional responses of fission yeast to environmental stress.
Chen D et al. Mol Biol Cell 2003 Jan;14(1):214-29
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:29996109 - Quantitative Phosphoproteomics Reveals the Signaling Dynamics of Cell-Cycle Kinases in the Fission Yeast Schizosaccharomyces pombe.
Swaffer MP et al. Cell Rep 2018 Jul 10;24(2):503-514
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
GO_REF:0000033 - Annotation inferences using phylogenetic trees