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protein coding gene - mcm7 (SPBC25D12.03c) - MCM complex subunit Mcm7

Gene summary

Standard name
mcm7
Systematic ID
SPBC25D12.03c
Product
MCM complex subunit Mcm7
Organism
Schizosaccharomyces pombe (fission yeast)
UniProt ID
O75001
ORFeome ID
33/33G03
Characterisation status
biological role published
Feature type
mRNA gene
Genomic location
chromosome II: 3717577..3720039 reverse strand

Annotation

Comment

PBO:0012045 - helicase activity of Mcm4/6/7 complex requires 5' overhang

References:

PBO:0017913 - Mcm2, Mcm4, and Mcm7 associate with chromatin simultaneously

References:

PBO:0024857 - MCM4/6/7 helicase complex binds a 3' overhang and translocates along single-stranded DNA in the 3' to 5' direction

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PBO:0024860 - MCM4/6/7 helicase complex binds a 3' single-stranded DNA overhang and translocates along an DNA/RNA duplex in the 3' to 5' direction

References:

PBO:0024859 - MCM4/6/7 helicase complex translocates along DNA/RNA duplex

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PBO:0024858 - MCM4/6/7 helicase complex translocates along double-stranded DNA

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PBO:0012046 - processive helicase activity of Mcm4/6/7 complex requires 3' and 5' overhangs

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GO biological process

GO:0006271 - DNA strand elongation involved in DNA replication

References:

GO:0000727 - double-strand break repair via break-induced replication

References:

GO:1902975 - mitotic DNA replication initiation

References:

GO:0006279 - premeiotic DNA replication

References:

GO cellular component

GO:0000785 - chromatin

References:

GO:0031261 - DNA replication preinitiation complex

References:

GO:0042555 - MCM complex

References:

GO:0097373 - MCM core complex

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GO:0005656 - nuclear pre-replicative complex

References:

GO:0043596 - nuclear replication fork

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GO:0005634 - nucleus

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GO molecular function

GO:0033679 - 3'-5' DNA/RNA helicase activity

References:

GO:0005524 - ATP binding

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GO:0016887 - ATP hydrolysis activity

References:

GO:1990518 - single-stranded 3'-5' DNA helicase activity

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GO:0003697 - single-stranded DNA binding

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GO:0003727 - single-stranded RNA binding

References:

GO:0003724 - RNA helicase activity

References:

Modification

MOD:00046 - O-phospho-L-serine

References:

MOD:01148 - ubiquitinylated lysine

References:

Qualitative gene expression

PomGeneEx:0000022 - protein absent

References:

PomGeneEx:0000023 - protein level constant

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PomGeneEx:0000019 - protein level decreased

References:

PomGeneEx:0000012 - RNA level decreased

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Quantitative gene expression

PBO:0006310 - protein level

References:

PBO:0011963 - RNA level

References:

Single locus phenotype

FYPO:0000848 - abnormal chromosome morphology during vegetative growth

References:

Genotypes:

FYPO:0000059 - abnormal mitotic cell cycle

References:

Genotypes:

FYPO:0000444 - abnormal mitotic cell cycle arrest with replicated DNA

References:

Genotypes:

FYPO:0001424 - abolished protein localization to nucleus during vegetative growth

References:

Genotypes:

FYPO:0000082 - decreased cell population growth at high temperature

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Genotypes:

FYPO:0001324 - decreased protein level during vegetative growth

References:

Genotypes:

FYPO:0001355 - decreased vegetative cell population growth

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Genotypes:

FYPO:0000614 - increased duration of mitotic S phase

References:

Genotypes:

FYPO:0000455 - increased number of double-strand break sites during vegetative growth

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Genotypes:

FYPO:0000972 - increased number of Rad52 foci during vegetative growth

References:

Genotypes:

FYPO:0000314 - inviable after spore germination with elongated germ tube

References:

Genotypes:

FYPO:0003529 - inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cells

References:

Genotypes:

FYPO:0000839 - inviable elongated mononucleate aseptate cell

References:

Genotypes:

FYPO:0002061 - inviable vegetative cell population

References:

Genotypes:

FYPO:0001387 - loss of viability at high temperature

References:

Genotypes:

FYPO:0001532 - normal duration of mitotic S phase

References:

Genotypes:

FYPO:0001420 - normal vegetative cell population growth rate

References:

Genotypes:

FYPO:0000783 - protein mislocalized to cytoplasm during vegetative growth

References:

Genotypes:

FYPO:0002060 - viable vegetative cell population

References:

Genotypes:

Taxonomic conservation

PBO:0011072 - conserved in archaea

PBO:0011065 - conserved in eukaryotes

PBO:0011064 - conserved in fungi

PBO:0011069 - conserved in metazoa

PBO:0011070 - conserved in vertebrates

PBO:0006222 - predominantly single copy (one to one)

Protein features

IDNameInterPro nameDB name
PF24901WHD_MCM7PFAM
PF17207MCM_OBMCM_OBPFAM
PF17855MCM_lidMCM_lidPFAM
PF00493MCMMCM_domPFAM
PF14551MCM_NMCM_NPFAM
cd17758MCM7MCM7CDD
PS00847MCM_1MCM_CSPROSITE_PATTERNS
PS50051MCM_2MCM_domPROSITE_PROFILES
SM00382AAA_5AAA+_ATPaseSMART
SM00350mcmMCMSMART
PR01657MCMFAMILYMCM_domPRINTS
PR01663MCMPROTEIN7MCM7PRINTS
G3DSA:2.20.28.10:FF:000004FUNFAM
G3DSA:3.40.50.300:FF:000288FUNFAM
SSF52540P-loop containing nucleoside triphosphate hydrolasesP-loop_NTPaseSUPERFAMILY
SSF50249Nucleic acid-binding proteinsNA-bd_OB-foldSUPERFAMILY
G3DSA:3.40.50.300P-loop_NTPaseGENE3D
G3DSA:3.30.1640.10GENE3D
G3DSA:2.40.50.140NA-bd_OB-foldGENE3D
G3DSA:2.20.28.10GENE3D
PTHR11630DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBERMCMPANTHER

Orthologs

References / Literature

PMID:26412298 - A Degenerate Cohort of Yeast Membrane Trafficking DUBs Mediates Cell Polarity and Survival.
Beckley JR et al. Mol Cell Proteomics 2015 Dec;14(12):3132-41
PMID:12972571 - Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding.
Gregan J et al. Mol Biol Cell 2003 Sep;14(9):3876-87
PMID:33823663 - A TOR (target of rapamycin) and nutritional phosphoproteome of fission yeast reveals novel targets in networks conserved in humans.
Halova L et al. Open Biol 2021 Apr;11(4):200405
PMID:37970674 - SUMOylation regulates Lem2 function in centromere clustering and silencing.
Strachan J et al. J Cell Sci 2023 Dec 01;136(23)
PMID:16899242 - Analysis of Mcm2-7 chromatin binding during anaphase and in the transition to quiescence in fission yeast.
Namdar M et al. Exp Cell Res 2006 Oct 15;312(17):3360-9
PMID:19714215 - The fission yeast homeodomain protein Yox1p binds to MBF and confines MBF-dependent cell-cycle transcription to G1-S via negative feedback.
Aligianni S et al. PLoS Genet 2009 Aug;5(8):e1000626
PMID:12975364 - Substrate requirements for duplex DNA translocation by the eukaryal and archaeal minichromosome maintenance helicases.
Shin JH et al. J Biol Chem 2003 Dec 05;278(49):49053-62
PMID:11854402 - Essential role of MCM proteins in premeiotic DNA replication.
Lindner K et al. Mol Biol Cell 2002 Feb;13(2):435-44
PMID:33313903 - Ribosome profiling reveals ribosome stalling on tryptophan codons and ribosome queuing upon oxidative stress in fission yeast.
Rubio A et al. Nucleic Acids Res 2021 Jan 11;49(1):383-399
PMID:15194812 - A novel intermediate in initiation complex assembly for fission yeast DNA replication.
Yamada Y et al. Mol Biol Cell 2004 Aug;15(8):3740-50
PMID:23322785 - The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.
Santosa V et al. J Biol Chem 2013 Mar 08;288(10):6864-80
PMID:12574132 - Multiple ORC-binding sites are required for efficient MCM loading and origin firing in fission yeast.
Takahashi T et al. EMBO J 2003 Feb 17;22(4):964-74
GO_REF:0000002 - Comments
PMID:22718908 - DNA polymerization-independent functions of DNA polymerase epsilon in assembly and progression of the replisome in fission yeast.
Handa T et al. Mol Biol Cell 2012 Aug;23(16):3240-53
PMID:10770926 - Isolation and characterization of various complexes of the minichromosome maintenance proteins of Schizosaccharomyces pombe.
Lee JK et al. J Biol Chem 2000 Jun 23;275(25):18871-8
GO_REF:0000033 - Annotation inferences using phylogenetic trees
GO_REF:0000117 - Electronic Gene Ontology annotations created by ARBA machine learning models
PMID:11973289 - Different phenotypes in vivo are associated with ATPase motif mutations in Schizosaccharomyces pombe minichromosome maintenance proteins.
Gómez EB et al. Genetics 2002 Apr;160(4):1305-18
PMID:23444842 - Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7.
Xu M et al. BMC Biochem 2013 Feb 27;14:5
PMID:18180284 - Minichromosome maintenance proteins interact with checkpoint and recombination proteins to promote s-phase genome stability.
Bailis JM et al. Mol Cell Biol 2008 Mar;28(5):1724-38
PMID:26771498 - A Proteome-wide Fission Yeast Interactome Reveals Network Evolution Principles from Yeasts to Human.
Vo TV et al. Cell 2016 Jan 14;164(1-2):310-323
PMID:20473289 - Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
Kim DU et al. Nat Biotechnol 2010 Jun;28(6):617-623
PMID:27611590 - Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
McDonald KR et al. PLoS Genet 2016 Sep;12(9):e1006238
PMID:30726745 - Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.
Tay YD et al. Cell Rep 2019 Feb 05;26(6):1654-1667.e7
PMID:11606752 - Redundant control of rereplication in fission yeast.
Gopalakrishnan V et al. Proc Natl Acad Sci U S A 2001 Nov 06;98(23):13114-9
PMID:21511999 - Comparative functional genomics of the fission yeasts.
Rhind N et al. Science 2011 May 20;332(6032):930-6
PMID:39367033 - Quantitative proteomics and phosphoproteomics profiling of meiotic divisions in the fission yeast Schizosaccharomyces pombe.
Sivakova B et al. Sci Rep 2024 Oct 04;14(1):23105
PMID:9366552 - A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast.
Adachi Y et al. Genes Cells 1997 Jul;2(7):467-79
PMID:22036784 - Purification and functional inactivation of the fission yeast MCM(MCM-BP) complex.
Li JJ et al. FEBS Lett 2011 Dec 15;585(24):3850-5
PMID:23101633 - Quantitative analysis of fission yeast transcriptomes and proteomes in proliferating and quiescent cells.
Marguerat S et al. Cell 2012 Oct 26;151(3):671-83
PMID:24763107 - Absolute proteome and phosphoproteome dynamics during the cell cycle of Schizosaccharomyces pombe (Fission Yeast).
Carpy A et al. Mol Cell Proteomics 2014 Aug;13(8):1925-36
PMID:9705352 - Purification of Hsk1, a minichromosome maintenance protein kinase from fission yeast.
Brown GW et al. J Biol Chem 1998 Aug 21;273(34):22083-90
PMID:11606526 - Characterization of Schizosaccharomyces pombe mcm7(+) and cdc23(+) (MCM10) and interactions with replication checkpoints.
Liang DT et al. Genetics 2001 Oct;159(2):471-86
PMID:11136247 - Processive DNA helicase activity of the minichromosome maintenance proteins 4, 6, and 7 complex requires forked DNA structures.
Lee JK et al. Proc Natl Acad Sci U S A 2001 Jan 02;98(1):54-9
PMID:16829518 - The replicative helicases of bacteria, archaea, and eukarya can unwind RNA-DNA hybrid substrates.
Shin JH et al. J Biol Chem 2006 Sep 15;281(37):26914-21
PMID:39094570 - A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Yu J et al. Cell 2024 Sep 05;187(18):5010-5028.e24
PMID:21712547 - Mitotic substrates of the kinase aurora with roles in chromatin regulation identified through quantitative phosphoproteomics of fission yeast.
Koch A et al. Sci Signal 2011 Jun 28;4(179):rs6
PMID:34108240 - The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes.
Liu Y et al. Proc Natl Acad Sci U S A 2021 Jun 15;118(24)
PMID:15338237 - Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.
Dolan WP et al. Chromosoma 2004 Sep;113(3):145-56
PMID:23697806 - A genome-wide resource of cell cycle and cell shape genes of fission yeast.
Hayles J et al. Open Biol 2013 May 22;3(5):130053
PMID:31262821 - Replication fork stalling elicits chromatin compaction for the stability of stalling replication forks.
Feng G et al. Proc Natl Acad Sci U S A 2019 Jul 16;116(29):14563-14572
PMID:12006645 - SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.
Nakajima R et al. Mol Biol Cell 2002 May;13(5):1462-72
PMID:21813639 - Schizosaccharomyces pombe minichromosome maintenance-binding protein (MCM-BP) antagonizes MCM helicase.
Ding L et al. J Biol Chem 2011 Sep 23;286(38):32918-30
PMID:18505873 - Schizosaccharomyces pombe histone acetyltransferase Mst1 (KAT5) is an essential protein required for damage response and chromosome segregation.
Gómez EB et al. Genetics 2008 Jun;179(2):757-71
PMID:25720772 - Quantitative phosphoproteomics reveals pathways for coordination of cell growth and division by the conserved fission yeast kinase pom1.
Kettenbach AN et al. Mol Cell Proteomics 2015 May;14(5):1275-87